INT221560

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Context Info
Confidence 0.72
First Reported 2007
Last Reported 2009
Negated 3
Speculated 1
Reported most in Body
Documents 36
Total Number 37
Disease Relevance 6.63
Pain Relevance 1.51

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

lipid binding (Fes) Golgi apparatus (Fes) plasma membrane (Fes)
cytoskeleton (Fes) kinase activity (Fes) cytoplasm (Fes)
Anatomy Link Frequency
brain 6
neuronal 3
upper 3
nervous system 2
neurons 2
Fes (Mus musculus)
Pain Link Frequency Relevance Heat
dorsal root ganglion 1332 100.00 Very High Very High Very High
Inflammation 2 95.96 Very High Very High Very High
cytokine 1 77.60 Quite High
Root ganglion neuron 36 71.92 Quite High
nociceptor 36 63.04 Quite High
Central nervous system 2 22.16 Low Low
withdrawal 36 5.00 Very Low Very Low Very Low
chemokine 2 5.00 Very Low Very Low Very Low
Pain 1 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Ganglion Cysts 1368 100.00 Very High Very High Very High
Diffuse Large B-cell Lymphoma 1 99.68 Very High Very High Very High
Shock 792 99.12 Very High Very High Very High
INFLAMMATION 2 95.96 Very High Very High Very High
Malignant Neoplastic Disease 1 94.08 High High
Lymphatic System Cancer 52 93.44 High High
Targeted Disruption 108 92.56 High High
Sprains And Strains 94 87.36 High High
Acquired Immune Deficiency Syndrome Or Hiv Infection 80 83.36 Quite High
Dementia 2 82.08 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
We next examined the expression of Fps and Fer specifically in DRGs dissected from neonatal mice (Fig. 1B).
Spec (examined) Gene_expression (expression) of Fps
1) Confidence 0.72 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
Comparable amounts of Fps plus Fer were present in these three stages of development (Fig. 2B, lower), and similar levels of in vitro kinase autophosphorylation were apparent (Fig. 2B, upper).
Gene_expression (present) of Fps in upper
2) Confidence 0.72 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
Subsequently these same authors showed that Fps was present at sites of microtubule nucleation in embryonic fibroblasts [21].
Gene_expression (present) of Fps in fibroblasts
3) Confidence 0.72 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.28 Pain Relevance 0.04
Since we detected substantially more Fer than Fps expression in the developing brain and DRG (Fig. 1), we decided to investigate the relative involvement of Fer and Fps in the Semaphorin signaling pathway.
Gene_expression (expression) of Fps in brain associated with dorsal root ganglion
4) Confidence 0.72 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.19 Pain Relevance 0.10
Collectively, these results show that Fer is expressed at substantially higher levels than Fps in whole neonatal mouse brain and DRGs; and furthermore, that Fer, but not Fps, is detectably tyrosine phosphorylated in vivo, and is therefore presumably active in neonatal brains.


Gene_expression (expressed) of Fps in brain
5) Confidence 0.72 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
In ferDR/DR and fpsKR/KR/ferDR/DR DRGs, approximately equal levels of Fps (or FpsKR) and FerDR proteins were detected.
Gene_expression (detected) of FpsKR
6) Confidence 0.72 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
Immunoblotting analysis revealed that Fer was expressed at much higher levels than Fps in wt and fpsKR/KR DRGs.
Gene_expression (expressed) of Fps
7) Confidence 0.72 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
HEK293T cells were transfected with Myc-epitope tagged Fps, FpsKR (kinase-dead), Fer or FerKR (kinase-dead) expression plasmids.
Gene_expression (expression) of Fps
8) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
Compared to wt, the Fps plus Fer kinase autophosphorylation was only slightly reduced in fps-/- lysates, substantially reduced in ferDR/DR, and abolished in fpsKR/KR/ferDR/DR lysates (Fig. 3A, upper).
Gene_expression (reduced) of Fps in upper
9) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.09 Pain Relevance 0
Fps and Fer kinases show highest levels of kinase activity in brain during perinatal stages of neuronal development; and there is more Fer than Fps activity
Gene_expression (activity) of Fps in neuronal
10) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
Fps-deficient DRG neurons displayed only a subtle defect in the Sema3A-induced axon retraction response
Gene_expression (neurons) of Fps-deficient in neurons associated with dorsal root ganglion
11) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.45 Pain Relevance 0.09
We have also found that Fyn is capable of phosphorylating kinase-inactive mutants of both Fps and Fer (data not shown).
Gene_expression (mutants) of Fps
12) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.08 Pain Relevance 0
A comparison of the relative amounts of Fps and Fer (Fig. 5A, lower) with the degree of CRMP2 tyrosine phosphorylation suggests that CRMP2 was a much better substrate for Fer than it was for Fps under these conditions.
Gene_expression (was) of Fps
13) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
Fps and Fer were not well resolved in these immunoprecipitates (Fig. 2B), relative to the lysates in Fig. 1.
Neg (not) Gene_expression (resolved) of Fps
14) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.06 Pain Relevance 0
In these in vitro assays, Fps was less active than Fer, but we could still see that Fps autophosphorylation activity was compromised by co-expression with PlexinA1.
Gene_expression (expression) of Fps
15) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.12 Pain Relevance 0.06
However, CRMP2 was not detected in these anti-Fps/Fer immunoprecipitates (data not shown).


Gene_expression (immunoprecipitates) of Fps
16) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.28 Pain Relevance 0.09
Interestingly, Mitsui and coworkers did not detect an association between Fps and CRAM or CRMP in their Cos-7 transfection studies; but they did observe an anti-Fps immunoreactive protein in CRAM immunoprecipitates from rat brains [20].
Gene_expression (immunoprecipitates) of Fps in brains
17) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.05 Pain Relevance 0.03
These assays were performed both in the presence and absence of ATP to control for the relatively low levels of in vivo tyrosine phosphorylation of Fps or Fer, which was not detectable under these conditions.
Neg (not) Gene_expression (detectable) of Fps
18) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.13 Pain Relevance 0.06
g/mL of recombinant Sema3A, we did see DRG growth cone collapse, but there was no significant differential response between wt and Fer- or Fps-deficient DRG neurons (data not shown).
Neg (no) Gene_expression (response) of Fps-deficient in neurons associated with dorsal root ganglion and shock
19) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.70 Pain Relevance 0.18
HEK293T cells were transfected with Myc-epitope tagged Fps, FpsKR (kinase-dead), Fer or FerKR (kinase-dead) expression plasmids.
Gene_expression (expression) of FpsKR
20) Confidence 0.63 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0

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