INT222028

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Context Info
Confidence 0.29
First Reported 2007
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 15
Total Number 17
Disease Relevance 3.65
Pain Relevance 1.46

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleus (Msc)
Anatomy Link Frequency
spinal cord 2
chondrocyte 1
tendon 1
Head 1
Msc (Rattus norvegicus)
Pain Link Frequency Relevance Heat
Spinal cord 649 99.16 Very High Very High Very High
Pain 167 75.68 Quite High
Inflammation 76 31.44 Quite Low
Osteoarthritis 9 13.64 Low Low
antagonist 24 5.00 Very Low Very Low Very Low
isoflurane 22 5.00 Very Low Very Low Very Low
imagery 22 5.00 Very Low Very Low Very Low
backache 15 5.00 Very Low Very Low Very Low
Inflammatory response 13 5.00 Very Low Very Low Very Low
Nerve growth factor 11 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Pulmonary Hypertension 300 99.20 Very High Very High Very High
Targeted Disruption 90 95.12 Very High Very High Very High
Spinal Cord Injury 99 87.68 High High
Rupture 15 87.64 High High
Hypoxia 14 84.48 Quite High
Disease 38 82.48 Quite High
Pain 166 75.68 Quite High
Hypertrophy 72 73.20 Quite High
Injury 44 68.80 Quite High
Cancer 154 67.36 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
HSR (RPT and MSC) is doctoral student and manual therapist, MBV (PhD) is associated professor in biostatistics, AMM (RPT and PhD) is professor, NKV (PhD) is professor and Head of institute

Pre-publication history

Gene_expression (professor) of MSC in Head
1) Confidence 0.29 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2890600 Disease Relevance 0.43 Pain Relevance 0.36
While in vivo EGFP expression of transplanted hMSC-EGFP cells was readily detectable at different time points post-transplantation (Figure 2B), in vivo transgene expression by hMSC-NT3 and hMSC-NT3-EGFP cells was, respectively, undetectable (Figure 3) or declined rapidly between day 1 and 7 post-transplantation (Figure 4).
Gene_expression (expression) of hMSC
2) Confidence 0.28 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC2225398 Disease Relevance 0.06 Pain Relevance 0.09
With regard to protein expression, Figure 1D shows a representative ELISA measurement of NT3 secretion by the different hMSC lines used in this study and Figure 1E shows a representative flow cytometric analysis of EGFP expression by the EGFP expressing hMSC lines used in this study.


Gene_expression (expressing) of hMSC
3) Confidence 0.28 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC2225398 Disease Relevance 0.16 Pain Relevance 0.05
The latter was recently also suggested by Eliopoulos et al.[26], who clearly demonstrated that mice implanted with MHC-mismatched MSC-expressing murine erythropoietin, in contrast to mice implanted with syngeneic MSC-expressing erythropoietin, failed in causing a sustained rise in hematocrit level.
Gene_expression (expressing) of MSC
4) Confidence 0.28 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC2225398 Disease Relevance 0.08 Pain Relevance 0.07
In order to investigate whether our established hMSC line has immune modulating properties, two series of transplantation experiments were performed using hMSC genetically modified with the EGFP reporter gene (Figure 1).
Gene_expression (using) of hMSC
5) Confidence 0.25 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC2225398 Disease Relevance 0.12 Pain Relevance 0.09
Flow cytometric analysis was used for routine (weekly) and pre-transplant measurement of EGFP transgene expression and cell viability of harvested genetically modified hMSC populations.
Gene_expression (expression) of hMSC
6) Confidence 0.25 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC2225398 Disease Relevance 0 Pain Relevance 0.03
In a next attempt to genetically modify hMSC to produce the NT3 neurothrophic factor, we used a DNA plasmid encoding both the NT3 and EGFP protein (Figure 1A, pIRES2-NT3-EGFP plasmid).
Gene_expression (produce) of hMSC
7) Confidence 0.25 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC2225398 Disease Relevance 0.27 Pain Relevance 0.12
Following genetic modification of hMSC with a DNA plasmid encoding the EGFP reporter gene and successful in vivo transplantation and detection of hMSC-EGFP cells, we genetically modified hMSC with a similar DNA plasmid-encoding rat NT3 (see Figure 1A, pCMV-NT3 plasmid).
Gene_expression (detection) of hMSC
8) Confidence 0.22 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC2225398 Disease Relevance 0 Pain Relevance 0.18
The latter was recently also suggested by Eliopoulos et al.[26], who clearly demonstrated that mice implanted with MHC-mismatched MSC-expressing murine erythropoietin, in contrast to mice implanted with syngeneic MSC-expressing erythropoietin, failed in causing a sustained rise in hematocrit level.
Gene_expression (expressing) of MSC
9) Confidence 0.22 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC2225398 Disease Relevance 0.08 Pain Relevance 0.04
Before transplantation experiments were carried out, transgene expression of these genetically modified hMSC lines was characterised at several passages during culture by PCR, real-time PCR, ELISA and flow cytometry.
Gene_expression (expression) of hMSC
10) Confidence 0.22 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC2225398 Disease Relevance 0.22 Pain Relevance 0.08
In this context, the aim of this study was to investigate the feasibility of a plasmid-based strategy for genetic modification of human (h)MSC with enhanced green fluorescent protein (EGFP) and/or neurotrophin (NT)3, and to study in vivo cell survival and transgene expression of genetically modified hMSC following transplantation in rat spinal cord.
Gene_expression (expression) of hMSC in spinal cord associated with spinal cord
11) Confidence 0.22 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC2225398 Disease Relevance 0.22 Pain Relevance 0.18
Next, we examined the stability of EGFP or NT3 transgene expression following transplantation of hMSC-EGFP, hMSC-NT3 and hMSC-NT3-EGFP in rat spinal cord.
Gene_expression (expression) of hMSC in spinal cord associated with spinal cord
12) Confidence 0.22 Published 2007 Journal BMC Biotechnol Section Abstract Doc Link PMC2225398 Disease Relevance 0.06 Pain Relevance 0.18
Gulotta et al. [27] found no difference in the histological production of an enthesis in 2 groups of tendon repairs, one with and the other without MSC injection during repair.
Gene_expression (injection) of MSC in tendon
13) Confidence 0.17 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923611 Disease Relevance 0 Pain Relevance 0
Improvement in overall healing rate by chondrocyte and MSC cell therapy
Gene_expression (therapy) of MSC in chondrocyte
14) Confidence 0.17 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923611 Disease Relevance 0.09 Pain Relevance 0
Restoration of a native-like enthesis by MSC-injection
Gene_expression (enthesis) of MSC
15) Confidence 0.17 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923611 Disease Relevance 0.11 Pain Relevance 0
One week after MCT administration, the rats received 3 different treatments: MSCs (MSC group), MSCs/eNOS, (MSC/eNOS group), or no treatment (PAH group).
Gene_expression (group) of MSC associated with pulmonary hypertension
16) Confidence 0.14 Published 2010 Journal Journal of Biomedicine and Biotechnology Section Body Doc Link PMC2843902 Disease Relevance 0.88 Pain Relevance 0
One week after MCT administration, the rats received 3 different treatments: MSCs (MSC group), MSCs/eNOS, (MSC/eNOS group), or no treatment (PAH group).
Gene_expression (group) of MSC associated with pulmonary hypertension
17) Confidence 0.14 Published 2010 Journal Journal of Biomedicine and Biotechnology Section Body Doc Link PMC2843902 Disease Relevance 0.88 Pain Relevance 0

General Comments

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