INT222635

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Context Info
Confidence 0.39
First Reported 2008
Last Reported 2010
Negated 0
Speculated 1
Reported most in Body
Documents 4
Total Number 24
Disease Relevance 15.20
Pain Relevance 0.20

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

extracellular space (Thbs1) cytoplasm (Thbs1)
Anatomy Link Frequency
astrocytes 10
brains 2
nervous system 2
spines 2
extracellular matrix 2
Thbs1 (Rattus norvegicus)
Pain Link Frequency Relevance Heat
Gabapentin 21 73.48 Quite High
cytokine 87 69.92 Quite High
substance P 48 64.72 Quite High
Somatostatin 18 62.00 Quite High
antagonist 117 41.84 Quite Low
agonist 66 40.56 Quite Low
imagery 42 32.72 Quite Low
Inflammation 81 29.00 Quite Low
metalloproteinase 24 13.60 Low Low
Nerve growth factor 84 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Syndrome 2544 99.84 Very High Very High Very High
Cancer 87 98.92 Very High Very High Very High
Stress 228 98.08 Very High Very High Very High
Apoptosis 24 96.68 Very High Very High Very High
Stroke 42 89.28 High High
Pituitary Cancer 33 82.80 Quite High
Parkinson's Disease 84 82.36 Quite High
Congenital Anomalies 84 79.00 Quite High
Cognitive Disorder 84 76.40 Quite High
Generaliased Trisomy 21 67.12 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Human astrocytes synthesize and secrete TSP-1, an extracellular matrix component involved in cell-cell and cell-matrix communication [26], [78], which is widely expressed in the developing nervous system [27].
Gene_expression (expressed) of TSP-1 in nervous system
1) Confidence 0.39 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.37 Pain Relevance 0
Given that TSP-1 is one of the most abundant proteins secreted by human astrocytes (data not shown), and that there are marked deficits in protein secretion in DS astrocytes [22], we next analyzed TSP-1 expression and secretion in DS astrocyte cultures and brain homogenates.
Spec (analyzed) Gene_expression (expression) of TSP-1 in astrocytes associated with syndrome
2) Confidence 0.39 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.85 Pain Relevance 0
A comparison of TSP-1 protein expression in fetal brain lysates showed an average reduction of 57.9% in TSP-1 levels in DS brains despite the average age of DS brain samples being almost 4 weeks younger than the average age of NL brain samples (Figure 3F). qPCR analysis of mRNA expression indicated similar mRNA levels in DS brains than in NL brains for all thrombospondin isoforms (TSP-1, -2, -3 and -4)(Figure S2), suggesting that a post transcriptional mechanism underlies the changes observed in TSP-1 in DS brains and astrocyte cultures.


Gene_expression (expression) of TSP-1 in brains associated with syndrome
3) Confidence 0.39 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.89 Pain Relevance 0
Human astrocytes synthesize and secrete TSP-1, an extracellular matrix component involved in cell-cell and cell-matrix communication [26], [78], which is widely expressed in the developing nervous system [27].
Gene_expression (synthesize) of TSP-1 in nervous system
4) Confidence 0.39 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.34 Pain Relevance 0
We found high TSP-1 expression in human astrocytes and prominent surface localization (Figure 3A-D).
Gene_expression (expression) of TSP-1 in astrocytes
5) Confidence 0.39 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.84 Pain Relevance 0
We also show that thrombospondin 1 (TSP-1), an astrocyte-secreted protein, possesses a potent modulatory effect on spine number and morphology, and that both DS brains and DS astrocytes exhibit marked deficits in TSP-1 protein expression.
Gene_expression (expression) of TSP-1 in brains associated with syndrome
6) Confidence 0.39 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2996288 Disease Relevance 1.02 Pain Relevance 0
These results demonstrate that soluble TSP-1 modulates spine morphology and that reduced expression of TSP-1 in DS astrocytes leads to abnormal spine development.


Gene_expression (expression) of TSP-1 in astrocytes associated with syndrome
7) Confidence 0.39 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.18 Pain Relevance 0
Antioxidants and mitochondrial cofactors have no effect on TSP-1 expression and secretory deficits in DS astrocytes
Gene_expression (expression) of TSP-1 in astrocytes associated with syndrome
8) Confidence 0.39 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 1.00 Pain Relevance 0
Quantification of TSP-1 protein levels by ELISA demonstrated significant reductions in both DS astrocyte cell lysates and CM (Figure 3E), suggesting that reduced TSP-1 expression leads to decreased TSP-1 in DS CM.
Gene_expression (expression) of TSP-1 in astrocyte associated with syndrome
9) Confidence 0.39 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.90 Pain Relevance 0
TSP-1 expression was considerably higher in fetal than in adult brain homogenates (data not shown), consistent with a significant role of TSP-1 during development [27].
Gene_expression (expression) of TSP-1 in adult brain
10) Confidence 0.39 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.89 Pain Relevance 0
TSP-1 is an extracellular matrix protein synthesized and released by astrocytes [26], which is highly expressed during development of the nervous system [27].
Gene_expression (synthesized) of TSP-1 in extracellular matrix
11) Confidence 0.39 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.77 Pain Relevance 0
Altered spine morphology in neurons growing on top of TSP-1 KO astrocytes
Gene_expression (astrocytes) of TSP-1 KO in neurons
12) Confidence 0.34 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.17 Pain Relevance 0
Reduced TSP-1 levels in DS astrocytes and DS brains
Gene_expression (levels) of TSP-1 in astrocytes associated with syndrome
13) Confidence 0.34 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.67 Pain Relevance 0
For some experiments, the following compounds (purchased from Sigma, St Louis, MO) were added to pure astrocyte cultures, and TSP-1 levels were determined after 24 hr: s-PBN (100 mM), Trolox (100 ┬ÁM), resveratrol (100 mM), nicotinamide (15 mM), nicotinamide adenine dinucleotide (?
Gene_expression (levels) of TSP-1 in astrocyte
14) Confidence 0.34 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0 Pain Relevance 0
To establish whether reduced expression of TSP-1 in DS astrocytes was associated with oxidative stress and/or energy deficits in DS cells, we analyzed the effect of free radical scavengers, antioxidants, and energy substrates on TSP-1 expression.
Gene_expression (expression) of TSP-1 in astrocytes associated with stress and syndrome
15) Confidence 0.30 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 1.11 Pain Relevance 0
One possibility is that mechanism(s) regulating TSP-1 expression such as activation of purinergic receptors [97], muscarinic receptors [74], or nitric oxide-mediated signaling [89], [98] could be altered in DS astrocytes.
Gene_expression (expression) of TSP-1 in astrocytes associated with syndrome
16) Confidence 0.30 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.93 Pain Relevance 0.10
TSP-1 is an extracellular matrix protein synthesized and released by astrocytes [26], which is highly expressed during development of the nervous system [27].
Gene_expression (expressed) of TSP-1 in extracellular matrix
17) Confidence 0.30 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.79 Pain Relevance 0
Spine and synaptic alterations were associated with reduced expression of TSP-1, which was also significantly reduced in DS brains.
Gene_expression (expression) of TSP-1 in Spine associated with syndrome
18) Confidence 0.30 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0.89 Pain Relevance 0.03
To establish whether reduced expression of TSP-1 in DS astrocytes was associated with oxidative stress and/or energy deficits in DS cells, we analyzed the effect of free radical scavengers, antioxidants, and energy substrates on TSP-1 expression.
Gene_expression (expression) of TSP-1 in astrocytes associated with stress and syndrome
19) Confidence 0.30 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 1.22 Pain Relevance 0
TSP-1 levels in the supernatant fraction were determined using a commercial ELISA kit following the vendor's instructions (R&D systems, Minneapolis, MN).
Gene_expression (levels) of TSP-1
20) Confidence 0.30 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2996288 Disease Relevance 0 Pain Relevance 0

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