INT223495

Context Info Confidence 0.05 First Reported 2007 Last Reported 2007 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 18 Disease Relevance 13.54 Pain Relevance 2.71

nucleolus (Imp3) RNA binding (Imp3) rRNA binding (Imp3) nucleus (Imp3) intracellular (Imp3) ribosome biogenesis (Imp3)

Anatomy Link Frequency lung 1 Imp3 (Mus musculus)

Pain Link Frequency Relevance Heat anesthesia 144 98.48 rheumatoid arthritis 36 49.36 Multiple sclerosis 54 43.32 Inflammation 36 37.80 Inflammatory response 18 25.24 Demyelination 18 9.76

Disease Link Frequency Relevance Heat Burns 1638 99.72 Contagious Ecthyma 36 99.36 Stress 234 93.52 Sprains And Strains 18 72.68 Injury 90 59.28 Disease 126 58.56 T-cell Lymphoma 18 51.16 Leukemia 36 50.32 Diabetes Mellitus-type I 18 49.60 Rheumatoid Arthritis 36 49.36

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

It was of interest to note that the U3 expression profiles in all three tissues of no burn mice (subjected to anesthesia and resuscitation only) at 3 hours were significantly different from the profiles at 24 hours.

Gene_expression (expression) of U3 associated with anesthesia and burns

1) Confidence 0.05 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 1.23 Pain Relevance 0.17

These findings suggest that the initial treatments (anesthesia and resuscitation) during the burn procedure contributed to changes in the U3 expression profiles in a tissue-specific manner.

Gene_expression (expression) of U3 associated with anesthesia and burns

2) Confidence 0.05 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.95 Pain Relevance 0.18

Furthermore, the genomic U3 profile was distantly related to the U3 expression profiles from both burn and no burn mice, including mice without any treatment.

Gene_expression (expression) of U3 associated with burns

3) Confidence 0.05 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.85 Pain Relevance 0.17

In addition, the U3 profiles of no burn mice at both time points did not match corresponding control tissues harvested without any treatment.

Gene_expression (profiles) of U3 associated with burns

4) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 1.11 Pain Relevance 0.18

Comparison analysis of the sizes of differentially expressed U3 fragments (Figure 1) and U3 clones provided information regarding the origins of these U3 clones.

Gene_expression (expressed) of U3

5) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.55 Pain Relevance 0

Furthermore, the genomic U3 profile was distantly related to the U3 expression profiles from both burn and no burn mice, including mice without any treatment.

Gene_expression (expression) of U3 associated with burns

6) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.86 Pain Relevance 0.18

Further characterization of the specific roles of the transcription regulatory elements predominantly present in the U3 sequences from either burn or no burn mice in burn-elicited systemic pathogenesis is warranted.

Gene_expression (present) of U3 associated with burns

7) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.55 Pain Relevance 0

In addition, 13 putative MuERVs (marked with "b") harbored U3 sequences matching 100 % to their respective U3 sequences isolated from no burn mice.

Gene_expression (sequences) of U3 associated with burns

8) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.50 Pain Relevance 0

In silico cloning/mapping of putative MuERVs using U3 sequences as a probe and ORF analysis

Gene_expression (sequences) of U3 associated with contagious ecthyma

9) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.15 Pain Relevance 0

In addition, the U3 expression profiles of tissues without any treatment were substantially different from no burn controls at both time points.

Gene_expression (expression) of U3 associated with burns

10) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.64 Pain Relevance 0.29

In addition, substantial differences in U3 expression profiles were observed in lung samples at 3 hours after burn (Figure 1B).

Gene_expression (expression) of U3 in lung associated with burns

11) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.74 Pain Relevance 0.23

Changes in the U3 expression profiles in all three tissues were evident at 24 hours after burn and these changes were tissue-specific.

Gene_expression (expression) of U3 associated with burns

12) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.84 Pain Relevance 0.18

In addition, five U3 sequences in group I (K-3-3 U3, K-3-6 U3, K-3-8 U3, L-3-5 U3, and U-1-7 U3) were presumed to originate from the ~700 bp fragments (before trimming 120 bp non-U3 sequences) whose expression was evident in no burn control mice compared to burn mice (Figure 1).

Gene_expression (expression) of U3 associated with burns

13) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.73 Pain Relevance 0.07

The difference in the U3 expression profiles among no burn controls at two different time points and no treatment controls suggests that anesthesia and resuscitation transiently affected the expression of MuERVs.

Gene_expression (expression) of U3 associated with anesthesia and burns

14) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.65 Pain Relevance 0.25

These changes were manifested by either an increase or decrease in the expression of specific U3 regions after burn.

Gene_expression (expression) of U3 associated with burns

15) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.83 Pain Relevance 0.23

The U3 expression profiles of these individual tissues were compared to the genomic (C57BL/6J) profile as well as the expression profiles of corresponding tissues harvested without any treatment, such as anesthesia and resuscitation (no treatment control).

Gene_expression (expression) of U3 associated with anesthesia

16) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 1.04 Pain Relevance 0.18

Further comparison analysis of the RT-PCR data and U3 clones will elucidate the relations between each differentially expressed U3 fragment and U3 clones of different sizes examined in this study.

Gene_expression (expressed) of U3

17) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.59 Pain Relevance 0.09

Interestingly, the U3 expression profiles of no burn controls (treated with anesthesia and resuscitation) of all three tissues at 3 hours were different at 24 hours.

Gene_expression (expression) of U3 associated with anesthesia and burns

18) Confidence 0.04 Published 2007 Journal BMC Genomics Section Body Doc Link PMC2241634 Disease Relevance 0.73 Pain Relevance 0.30

General Comments

This test has worked.