INT226466

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.56
First Reported 2008
Last Reported 2008
Negated 2
Speculated 0
Reported most in Body
Documents 2
Total Number 23
Disease Relevance 4.51
Pain Relevance 1.14

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell adhesion (S1PR1) plasma membrane (S1PR1) signal transducer activity (S1PR1)
Anatomy Link Frequency
neural 3
neural tube 1
embryonic stem cell 1
Th2 cells 1
S1PR1 (Homo sapiens)
Pain Link Frequency Relevance Heat
Neurotransmitter 22 99.28 Very High Very High Very High
Inflammation 96 98.68 Very High Very High Very High
agonist 262 97.08 Very High Very High Very High
Dopamine 66 96.12 Very High Very High Very High
Clonidine 44 66.40 Quite High
cytokine 91 57.80 Quite High
dopamine receptor 44 57.20 Quite High
chemokine 23 36.12 Quite Low
Hippocampus 22 25.76 Quite Low
antagonist 34 24.48 Low Low
Disease Link Frequency Relevance Heat
Exencephaly /

Neural Tube Defects

66 99.36 Very High Very High Very High
INFLAMMATION 103 98.68 Very High Very High Very High
Bordatella Infection 110 98.48 Very High Very High Very High
Wound Healing 44 98.20 Very High Very High Very High
Apoptosis 97 97.44 Very High Very High Very High
Hyperplasia 9 91.84 High High
Cancer 69 89.76 High High
Asthma 315 89.12 High High
Viral Infection 1 85.92 High High
Neurodegenerative Disease 44 81.92 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Specifically, mice with genetic deletion of Sphingosine kinases required for production of S1P developed cranial neural tube defects as a result of increased apoptosis, decreased mitosis and subsequent thinning of the neuroepithelial progenitor cell layer [6].
Gene_expression (production) of S1P in neural tube associated with apoptosis
1) Confidence 0.56 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0.35 Pain Relevance 0
S1P1 receptor transcript was dramatically upregulated approximately forty fold in hES-NEP cells relative to the parent ES cell line (Figure 1B), while significant changes were not observed in expression of S1P 2, 3, and 5 transcript.


Gene_expression (expression) of S1P
2) Confidence 0.56 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0.21
LPA and S1P receptors are expressed in neural progenitors, neurons, and oligodendrocytes in the developing and adult brain, and both LPA and S1P are generated by neurons [9-11].
Gene_expression (expressed) of S1P in neural
3) Confidence 0.56 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0.42 Pain Relevance 0
Similarly, S1P 1, 2, and 3 transcripts were expressed at significantly higher levels in hES-NEP cells than S1P5.
Gene_expression (expressed) of S1P 1
4) Confidence 0.56 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0
We have determined that functional LPA and S1P receptors are expressed in hES-NEPs and regulate second messenger pathways, MAP kinase-dependent cell proliferation, and Rho-dependent morphology changes.
Gene_expression (expressed) of S1P
5) Confidence 0.56 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0.14 Pain Relevance 0
To evaluate expression of GPCRs for LPA and S1P as well as major neurotransmitter classes in hES-NEP cells, we screened agonists of adrenergic, dopamine, muscarinic acetylcholine, LPA, and S1P receptors for activity in assays measuring second messenger production.
Gene_expression (expression) of S1P associated with dopamine, neurotransmitter and agonist
6) Confidence 0.56 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0.38
NEP cells express functional LPA and S1P receptors
Gene_expression (express) of S1P
7) Confidence 0.56 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0.31
We further determined the fold change in transcript expression of LPA1, 2, 4, and 5 and S1P 1, 2, 3, and 5 in hES-NEP cells relative to their expression in the parent ES cell line WA09.
Gene_expression (expression) of S1P 1
8) Confidence 0.56 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0
Expression data for each LPA or S1P receptor was first normalized against endogenous 18S ribosomal RNA within each cDNA, and then the relative expression in hES-NEP was compared to hES cells using the ??
Gene_expression (Expression) of S1P receptor
9) Confidence 0.56 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0
Expression data for each LPA or S1P receptor was first normalized against endogenous 18S ribosomal RNA within each cDNA, and then the relative expression in hES-NEP was compared to hES cells using the ??
Gene_expression (normalized) of S1P receptor
10) Confidence 0.48 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0
LPA and S1P induce reversible morphological changes in hES-NEP cells
Gene_expression (induce) of S1P
11) Confidence 0.48 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0
LPA or S1P was then applied to the cells for the indicated period of time.
Gene_expression (applied) of S1P
12) Confidence 0.48 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0
Our data clearly implicate Rho-mediated activation of ROCK in mediating LPA and S1P stimulated rounding and aggregation in hES-NEP cells.
Gene_expression (rounding) of S1P
13) Confidence 0.48 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0
Our results demonstrate that hES-NEP cells express functional LPA and S1P receptors coupled to Gi/o mediated inhibition of adenylyl cyclase and to a pertussis toxin-insensitive PLC pathway, likely mediated by Gq. hES-NEP cells do not express functional Gs coupled receptors for either LPA or S1P.
Gene_expression (express) of S1P associated with bordatella infection
14) Confidence 0.43 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0.35 Pain Relevance 0
There is strong evidence that both LPA and S1P are critical in early neural development, as mouse embryos that lack enzymes for S1P or LPA synthesis exhibit severe neural tube defects.
Gene_expression (synthesis) of S1P in neural
15) Confidence 0.43 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0.34 Pain Relevance 0
LPA and S1P receptors are expressed in neural progenitors, neurons, and oligodendrocytes in the developing and adult brain, and both LPA and S1P are generated by neurons [9-11].
Gene_expression (generated) of S1P in neural
16) Confidence 0.43 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0.36 Pain Relevance 0
Our results demonstrate that Lysophosphatidic Acid (LPA) and Sphingosine-1-phosphate (S1P) receptors are functionally expressed in hES-NEP cells and are coupled to multiple cellular signaling pathways.
Gene_expression (expressed) of S1P
17) Confidence 0.43 Published 2008 Journal BMC Neurosci Section Abstract Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0
We have shown that transcript levels for S1P1 receptor increased significantly in the transition from embryonic stem cell to hES-NEP. hES-NEP cells express LPA and S1P receptors coupled to Gi/o G-proteins that inhibit adenylyl cyclase and to Gq-like phospholipase C activity.
Gene_expression (express) of S1P in embryonic stem cell
18) Confidence 0.43 Published 2008 Journal BMC Neurosci Section Abstract Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0
LPA or S1P was then applied for an additional 18 hours.
Gene_expression (applied) of S1P
19) Confidence 0.42 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0
In our experiments, LPA or S1P were added to the media and not washed out throughout the experiment.
Neg (not) Gene_expression (added) of S1P
20) Confidence 0.42 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2621239 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox