INT227102

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.77
First Reported 2008
Last Reported 2008
Negated 0
Speculated 1
Reported most in Body
Documents 2
Total Number 37
Disease Relevance 9.58
Pain Relevance 0.26

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

phosphatase activity (Mtm1) endosome (Mtm1) transport (Mtm1)
plasma membrane (Mtm1) cytoplasm (Mtm1)
Anatomy Link Frequency
muscle 13
skeletal muscle 8
plasma 2
muscle fibers 2
EDL 2
Mtm1 (Mus musculus)
Pain Link Frequency Relevance Heat
Inflammation 72 98.38 Very High Very High Very High
Sciatic nerve 36 97.32 Very High Very High Very High
sodium channel 36 48.20 Quite Low
ketamine 36 5.00 Very Low Very Low Very Low
anesthesia 36 5.00 Very Low Very Low Very Low
Hippocampus 2 5.00 Very Low Very Low Very Low
depression 1 5.00 Very Low Very Low Very Low
Opioid 1 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Congenital Structural Myopathies 612 99.80 Very High Very High Very High
Congenital Anomalies 39 99.76 Very High Very High Very High
Cytomegalovirus Infection 468 99.56 Very High Very High Very High
INFLAMMATION 72 98.38 Very High Very High Very High
Muscle Disease 180 97.72 Very High Very High Very High
Disease 181 95.48 Very High Very High Very High
Muscle Weakness 144 95.04 Very High Very High Very High
Muscular Dystrophy 36 92.28 High High
Adhesions 38 83.36 Quite High
Neuropathic Pain 36 77.28 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Apparently low levels of myotubularin expression in skeletal muscle had previously precluded determination of myotubularin’s subcellular localization in this tissue.
Gene_expression (expression) of myotubularin in skeletal muscle
1) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.23 Pain Relevance 0.03
Thus, it may be possible that excessive caveolae formation upon myotubularin overexpression contributes to the formation of these membrane structures.
Gene_expression (overexpression) of myotubularin
2) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.18 Pain Relevance 0
Myotubularin is a ubiquitously expressed phosphoinositide phosphatase with a muscle-specific role in man and mouse that is poorly understood.
Gene_expression (expressed) of Myotubularin in muscle
3) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Abstract Doc Link PMC2441725 Disease Relevance 0.35 Pain Relevance 0
In addition, we used this AAV vector to overexpress myotubularin in wild-type skeletal muscle and get insight into its localization and function.
Gene_expression (overexpress) of myotubularin in skeletal muscle
4) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Abstract Doc Link PMC2441725 Disease Relevance 0.28 Pain Relevance 0
Myotubularin overexpression in muscle induces the accumulation of packed membrane saccules and presence of vacuoles that contain markers of sarcolemma and T-tubules, suggesting that myotubularin is involved in plasma membrane homeostasis of myofibers.
Gene_expression (overexpression) of Myotubularin in plasma
5) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Abstract Doc Link PMC2441725 Disease Relevance 0.25 Pain Relevance 0
Like TA, the EDL showed a clear increase in muscle mass (5.7 ± 0.5 mg in mKO-AAV compared with 3.6 ± 0.1 mg in mKO-PBS EDL muscle, n = 6 for each group, P < 0.0006), indicating that AAV transduction and myotubularin expression were also efficient in this muscle, see Figure 4A.
Gene_expression (expression) of myotubularin in EDL
6) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.30 Pain Relevance 0.03
Figure 2B shows that myotubularin re-expression in Mtm1-deficient muscle leads to a doubling in muscle mass (26.5 ± 3.1 mg in transduced compared with 12.3 ± 1.7 mg in mKO TA) that correlates with an increase in the volume of myofibers.
Gene_expression (expression) of myotubularin in muscle
7) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.21 Pain Relevance 0
However, development of the new r1947 antibody, together with the use of AAV-mediated transduction to overexpress myotubularin, permitted us to demonstrate that it preferentially localizes to the triadic region, and perhaps also to the sarcolemma.
Gene_expression (overexpress) of myotubularin
8) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.20 Pain Relevance 0.03
Importantly, viral-mediated myotubularin expression led to the correction of EDL and TA muscle-specific forces in mutant mice, reaching a level similar to WT animals (Fig. 4B).
Gene_expression (expression) of myotubularin in muscle
9) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.18 Pain Relevance 0
Subcellular fractionation studies provided further confirmation that the localization of overexpressed myotubularin in muscle likely corresponds to that of the endogenous protein as they revealed a similar pattern of distribution.
Gene_expression (overexpressed) of myotubularin in muscle
10) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0 Pain Relevance 0
These results suggest that overexpressed myotubularin has a similar distribution than the endogenous protein within muscle fibers and validate the use of AAV-mediated overexpression for functional studies.


Gene_expression (overexpressed) of myotubularin in muscle fibers
11) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.07 Pain Relevance 0
Overexpressed myotubularin, like the endogenous one, is present as a doublet in skeletal muscle by western blot (Fig. 5D), indicating that these isoforms result from post-translational modification of the protein rather than alternative splicing of the Mtm1 gene.
Gene_expression (Overexpressed) of myotubularin in skeletal muscle
12) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.07 Pain Relevance 0
Myotubular myopathy, which affects one newborn male in 50 000 is caused by mutations in the ubiquitously expressed MTM1 gene that codes for a phosphoinositide phosphatase named myotubularin (2,3).
Gene_expression (expressed) of MTM1 associated with congenital structural myopathies
13) Confidence 0.77 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.58 Pain Relevance 0
Occasional small foci of inflammatory infiltrates were observed in AAV-transduced mKO muscles, often nearby myofibers expressing high levels of myotubularin (Fig. 3C).
Gene_expression (expressing) of myotubularin in muscles associated with inflammation
14) Confidence 0.67 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.36 Pain Relevance 0.05
Confocal microscopic examination of AAV transduced muscle overexpressing myotubularin revealed considerably stronger signal at the triads and specific localization at the sarcolemma as well.
Gene_expression (overexpressing) of myotubularin in muscle
15) Confidence 0.67 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.06 Pain Relevance 0
We also compared the distribution of endogenous and overexpressed myotubularin in skeletal muscle by subcellular fractionation.
Gene_expression (overexpressed) of myotubularin in skeletal muscle
16) Confidence 0.67 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.06 Pain Relevance 0
Overexpressed myotubularin induces a plasma membrane pathology in skeletal muscle
Gene_expression (Overexpressed) of myotubularin in plasma
17) Confidence 0.67 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.08 Pain Relevance 0
This study provides a proof-of-principle that local delivery of an AAV vector expressing myotubularin can improve the motor capacities of XLMTM muscle and represents a novel approach to study myotubularin function in skeletal muscle.



Gene_expression (expressing) of myotubularin in muscle
18) Confidence 0.67 Published 2008 Journal Human Molecular Genetics Section Abstract Doc Link PMC2441725 Disease Relevance 0.13 Pain Relevance 0
We have constructed an adeno-associated virus (AAV) vector expressing myotubularin in order to test its therapeutic potential in a XLMTM mouse model.
Gene_expression (expressing) of myotubularin
19) Confidence 0.67 Published 2008 Journal Human Molecular Genetics Section Abstract Doc Link PMC2441725 Disease Relevance 0.34 Pain Relevance 0
Figure 2B shows that myotubularin re-expression in Mtm1-deficient muscle leads to a doubling in muscle mass (26.5 ± 3.1 mg in transduced compared with 12.3 ± 1.7 mg in mKO TA) that correlates with an increase in the volume of myofibers.
Gene_expression (expression) of Mtm1 in muscle
20) Confidence 0.67 Published 2008 Journal Human Molecular Genetics Section Body Doc Link PMC2441725 Disease Relevance 0.21 Pain Relevance 0

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox