INT229463

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Context Info
Confidence 0.66
First Reported 2008
Last Reported 2008
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 13
Disease Relevance 1.87
Pain Relevance 1.64

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (Ndel1) transport (Ndel1) nuclear envelope (Ndel1)
chromosome segregation (Ndel1) chromosome (Ndel1) cytoskeleton (Ndel1)
Anatomy Link Frequency
sciatic nerve 4
neuronal 3
neurons 1
nerves 1
Ndel1 (Mus musculus)
Pain Link Frequency Relevance Heat
Sciatic nerve 403 99.00 Very High Very High Very High
dorsal root ganglion 247 98.76 Very High Very High Very High
Spinal cord 130 90.88 High High
butorphanol 13 5.00 Very Low Very Low Very Low
Analgesic 13 5.00 Very Low Very Low Very Low
Dorsal column 13 5.00 Very Low Very Low Very Low
anesthesia 13 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Ganglion Cysts 286 98.76 Very High Very High Very High
Injury 273 97.68 Very High Very High Very High
Nervous System Injury 26 95.32 Very High Very High Very High
Targeted Disruption 39 94.72 High High
Neuroblastoma 13 80.52 Quite High
Coronary Artery Disease 39 77.72 Quite High
Schizophrenia 13 70.56 Quite High
Embryonic Lethality 26 31.64 Quite Low
Neurodegenerative Disease 13 5.12 Low Low
Infection 13 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The difference in upregulation of Ndel1 and Vimentin mRNA vs protein levels suggests that translation of these proteins is also regulated following sciatic nerve injury.
Positive_regulation (upregulation) of Ndel1 in sciatic nerve associated with nervous system injury and sciatic nerve
1) Confidence 0.66 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.18 Pain Relevance 0.22
The upregulation of soluble Ndel1 in injured nerves again led to enhanced formation of the neuronal Ndel1/full length Vimentin complex, as determined by co-immunoprecipitations with Ndel1 antibodies (Fig. 3B).
Positive_regulation (upregulation) of Ndel1 in neuronal
2) Confidence 0.66 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0 Pain Relevance 0.14
Using Z-stack sections of confocal microscopy, we further confirmed the upregulation of Ndel1 in injured regenerating axons (Fig. 3D and Fig.
Positive_regulation (upregulation) of Ndel1
3) Confidence 0.66 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.08 Pain Relevance 0.13
Remarkably, the upregulation of Ndel1 and full length Vimentin was strongly sustained in injured nerves even up to two weeks after injury (Fig. 2C).
Positive_regulation (upregulation) of Ndel1 in nerves associated with injury
4) Confidence 0.66 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.21 Pain Relevance 0.08
While Vimentin full length (FL) and reported truncated fragments were strongly upregulated, the augmentation in levels of Ndel1 protein was modest (Fig. 2A and 2B; Fig.
Positive_regulation (augmentation) of Ndel1 protein
5) Confidence 0.47 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.17 Pain Relevance 0.23
Using the McDonald “T” chamber, we locally silenced Ndel1 by siRNA in transected axons; we observed decreased regeneration in vivo (Fig. 6) in a manner reminiscent of, but to a more severe extent than sensory axons lacking Vimentin [27].
Positive_regulation (silenced) of Ndel1
6) Confidence 0.47 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.09 Pain Relevance 0
Upregulation of neuronal Ndel1/Vimentin complex correlates with regeneration after sciatic nerve transection
Positive_regulation (Upregulation) of Ndel1/Vimentin in sciatic nerve associated with sciatic nerve
7) Confidence 0.44 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.06 Pain Relevance 0.13
Similar to the crush model, we observed synthesis and upregulation of Ndel1 and full length Vimentin in both injured distal and proximal nerve stumps when compared to the non-injured contralateral sides (control) at 65 hours following sciatic nerve transection (Fig. 3A and B).
Positive_regulation (upregulation) of Ndel1 in sciatic nerve associated with sciatic nerve
8) Confidence 0.44 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0 Pain Relevance 0.14
Ndel1 was synthesized de novo and upregulated in crushed and transected sciatic nerve axons, and, upon injury, was strongly associated with neuronal form of the intermediate filament (IF) Vimentin while dissociating from the mature neuronal IF (Neurofilament) light chain NF-L.
Positive_regulation (upregulated) of Ndel1 in neuronal associated with injury and sciatic nerve
9) Confidence 0.44 Published 2008 Journal PLoS ONE Section Abstract Doc Link PMC2291557 Disease Relevance 0.33 Pain Relevance 0.05
Each gene (Ndel1, Vimentin, GAP-43, and GAPDH) was amplified for 33cycles. 10 µl aliquots of PCR products were electrophoresed on a 1.5 % agarose gel in Tri-acetate-EDTA (TAE) buffer and visualized by staining with etidium bromide (EtBr).
Positive_regulation (amplified) of Ndel1
10) Confidence 0.44 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0 Pain Relevance 0.04
Upregulation of neuronal Ndel1/Vimentin complex correlates with regeneration after sciatic nerve crush
Positive_regulation (Upregulation) of Ndel1/Vimentin in sciatic nerve associated with sciatic nerve
11) Confidence 0.44 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.07 Pain Relevance 0.28
We now discover that Ndel1 is upregulated at the mRNA and protein levels in injured axons in vivo and during regeneration, it preferentially associates with neuronal Vimentin.
Positive_regulation (upregulated) of Ndel1 in neuronal
12) Confidence 0.44 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.31 Pain Relevance 0.09
Silencing Ndel1 by RNAi in lesion conditioned DRG neurons reduces neurite outgrowth
Positive_regulation (Silencing) of Ndel1 in neurons associated with dorsal root ganglion
13) Confidence 0.41 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.37 Pain Relevance 0.12

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