INT229497

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Context Info
Confidence 0.33
First Reported 2008
Last Reported 2008
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 18
Disease Relevance 3.50
Pain Relevance 2.81

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytoplasm (Vim, Ndel1) cytoskeleton (Vim, Ndel1) cell differentiation (Ndel1)
transport (Ndel1) nuclear envelope (Ndel1) chromosome segregation (Ndel1)
Anatomy Link Frequency
neuronal 6
sciatic nerves 1
spinal cord 1
neurons 1
neurite 1
Vim (Mus musculus)
Ndel1 (Mus musculus)
Pain Link Frequency Relevance Heat
Sciatic nerve 558 100.00 Very High Very High Very High
Spinal cord 180 100.00 Very High Very High Very High
dorsal root ganglion 342 96.76 Very High Very High Very High
butorphanol 18 5.00 Very Low Very Low Very Low
Analgesic 18 5.00 Very Low Very Low Very Low
Dorsal column 18 5.00 Very Low Very Low Very Low
anesthesia 18 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Injury 378 100.00 Very High Very High Very High
Ganglion Cysts 396 96.76 Very High Very High Very High
Neuroblastoma 18 85.52 High High
Coronary Artery Disease 54 82.72 Quite High
Nervous System Injury 36 77.04 Quite High
Schizophrenia 18 75.56 Quite High
Targeted Disruption 54 70.12 Quite High
Neurodegenerative Disease 18 10.12 Low Low
Embryonic Lethality 36 7.04 Low Low
Infection 18 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Based on this knowledge, we reasoned that upon axotomy, Ndel1 will interact less with NF-L (in favor of Vimentin) in tissues with higher regenerative potential.
Vimentin Binding (interact) of Ndel1
1) Confidence 0.33 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.08 Pain Relevance 0.04
In tissues with higher regenerative capacity, Ndel1 preferentially associates with the neuronal form of Vimentin in detriment of NF-L.
Vimentin Binding (associates) of Ndel1 in neuronal
2) Confidence 0.33 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.24 Pain Relevance 0.05
Recently, we found that Ndel1 associates with Vimentin during neurite extension [23].
Vimentin Binding (associates) of Ndel1 in neurite
3) Confidence 0.33 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.49 Pain Relevance 0.31
As neuroregeneration is being completed and NFs are re-upregulated to replace Vimentin, the ability of Ndel1 to associate with both Vimentin and NFs may confer to this protein the unique capacity to substitute IFs.
Vimentin Binding (associate) of Ndel1
4) Confidence 0.33 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.27 Pain Relevance 0.04
For instance, through association with Vimentin and NF-L, Ndel1 may favor the formation of NF/Vimentin heteropolymers in injured axons, thereby serving as an initial site for polymerization of Neurofilaments to rebuild the IF network and stabilize the growing axons.
Vimentin Binding (association) of Ndel1
5) Confidence 0.33 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.27 Pain Relevance 0.04
These data support the ideas that Ndel1 is in complex with Vimentin in axons and neurons and the Ndel1 siRNA altered the functions of neuronal Vimentin.
Vimentin Binding (complex) of Ndel1 in neuronal
6) Confidence 0.33 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.16 Pain Relevance 0.05
Together, these results indicate that following injury, Ndel1 interacts differentialy with soluble neuronal Vimentin and NF-L in nervous tissues undergoing regeneration.


Vimentin Binding (interacts) of Ndel1 in neuronal associated with injury
7) Confidence 0.28 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.41 Pain Relevance 0.11
We found that truncated forms of Vimentin, but not the full length, co-immunoprecipitated with Ndel1 at these early time points (Fig. 2B; Fig.
Vimentin Binding (immunoprecipitated) of Ndel1
8) Confidence 0.25 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.21 Pain Relevance 0.11
The direct interaction between Ndel1 and Vimentin was demonstrated by an in vitro binding assay using purified His-Ndel1 and GST-Vimentin proteins.
Vimentin Binding (interaction) of Ndel1
9) Confidence 0.24 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.05 Pain Relevance 0.23
Furthermore, Vimentin also co-purified with Ndel1 in axonal cytoskeletal preparations from spinal cord and co-immunoprecipitated with Ndel1 in these preparations (Fig. 1F).
Vimentin Binding (immunoprecipitated) of Ndel1 in spinal cord associated with spinal cord
10) Confidence 0.24 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.06 Pain Relevance 0.30
Whether Ndel1 contributes to or impedes axon regeneration in vivo in association with Vimentin remains unknown.
Vimentin Binding (association) of Ndel1
11) Confidence 0.24 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.32 Pain Relevance 0.09
In these conditions, Vimentin nicely co-immunoprecipitated with Ndel1 from PBS-isolated axoplasm of sciatic nerves (Fig. 1E).
Vimentin Binding (immunoprecipitated) of Ndel1 in sciatic nerves associated with sciatic nerve
12) Confidence 0.24 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.12 Pain Relevance 0.36
The upregulation of soluble Ndel1 in injured nerves again led to enhanced formation of the neuronal Ndel1/full length Vimentin complex, as determined by co-immunoprecipitations with Ndel1 antibodies (Fig. 3B).
Vimentin Binding (complex) of Ndel1 in neuronal
13) Confidence 0.24 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0 Pain Relevance 0.13
The upregulation of soluble Ndel1 in injured nerves again led to enhanced formation of the neuronal Ndel1/full length Vimentin complex, as determined by co-immunoprecipitations with Ndel1 antibodies (Fig. 3B).
Vimentin Binding (complex) of Ndel1 in neuronal
14) Confidence 0.24 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0 Pain Relevance 0.13
Furthermore, using multiple biochemical and cellular methods, Ndel1 was found to associate with Vimentin in axons (Figs. 2 and 3).
Vimentin Binding (associate) of Ndel1
15) Confidence 0.24 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.14 Pain Relevance 0.03
Taken together, these results indicate that Ndel1 and Vimentin selectively co-associate in a subset of axons in vivo.
Vimentin Binding (associate) of Ndel1
16) Confidence 0.24 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0 Pain Relevance 0.24
To seek further evidence for an in vivo association between Ndel1 and neuronal Vimentin in neurons, we isolated axoplasms and axonal preparations from sciatic nerve and spinal cord, respectively.
Vimentin Binding (association) of Ndel1 in neurons associated with sciatic nerve and spinal cord
17) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.34 Pain Relevance 0.48
Ndel1 was synthesized de novo and upregulated in crushed and transected sciatic nerve axons, and, upon injury, was strongly associated with neuronal form of the intermediate filament (IF) Vimentin while dissociating from the mature neuronal IF (Neurofilament) light chain NF-L.
Vimentin Binding (associated) of Ndel1 in neuronal associated with injury and sciatic nerve
18) Confidence 0.21 Published 2008 Journal PLoS ONE Section Abstract Doc Link PMC2291557 Disease Relevance 0.36 Pain Relevance 0.05

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