INT230159

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Context Info
Confidence 0.76
First Reported 2008
Last Reported 2008
Negated 2
Speculated 0
Reported most in Body
Documents 37
Total Number 37
Disease Relevance 1.17
Pain Relevance 0

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

lipid binding (OSBP) transport (OSBP) Golgi apparatus (OSBP)
cytoplasm (OSBP)
Anatomy Link Frequency
plasma 2
brain 1
OSBP (Homo sapiens)
Pain Link Frequency Relevance Heat
agonist 36 46.92 Quite Low
metalloproteinase 1 33.52 Quite Low
Kinase C 39 23.60 Low Low
cINOD 108 5.00 Very Low Very Low Very Low
Dopamine 2 5.00 Very Low Very Low Very Low
Central nervous system 1 5.00 Very Low Very Low Very Low
anesthesia 1 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Disease 324 81.56 Quite High
Alzheimer's Dementia 180 78.24 Quite High
Adhesions 37 73.44 Quite High
Disorder Of Lipid Metabolism 1 56.80 Quite High
Myopia 22 50.00 Quite Low
Hyperopia 9 49.44 Quite Low
Stress 36 5.00 Very Low Very Low Very Low
INFLAMMATION 36 5.00 Very Low Very Low Very Low
Sarcoma 1 5.00 Very Low Very Low Very Low
Overdose 1 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The effect of OSBP1 overexpression on A?
Gene_expression (overexpression) of OSBP1
1) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
were observed with OSBP1 overexpression in H4-APP cells (Figure 2A).
Gene_expression (overexpression) of OSBP1
2) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
When OSBP1 was not overexpressed, 25OH had no effect on the localization of the APP-Notch2 dimers, perhaps because basal levels of OSBP1 were not high enough to counteract the effects of 25OH binding to other ORPs or LXRs.
Neg (not) Gene_expression (overexpressed) of OSBP1
3) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0.06 Pain Relevance 0
Co-expressing OSBP1 with an APP-GFP construct indicated that OSBP1 did not affect the gross intracellular distribution of APP, in the presence or absence of OSBP1 ligand 25OH (Figure 4A).
Gene_expression (expressing) of OSBP1
4) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
We also performed sucrose gradient fractionation of APP-overexpressing cells transiently transfected with OSBP1 or empty vector (Figure 4B and 4C).
Gene_expression (transfected) of OSBP1
5) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
In addition, OSBP1 overexpression was unable to further alter the concomitant increase in APP-CTF?
Gene_expression (overexpression) of OSBP1
6) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
H4 cells were transfected with APPYC plus N2C50YN in the absence or presence of overexpressed OSBP1 and then treated with 1 ?
Gene_expression (overexpressed) of OSBP1
7) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
However, by using bimolecular fluorescence complementation to study the movement of APP dimers, we found that OSBP1 overexpression caused sequestration of APP-Notch2 dimers in the Golgi.
Gene_expression (overexpression) of OSBP1
8) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0.07 Pain Relevance 0
APP was found throughout fractions 4–12, and this distribution was not altered by overexpression of OSBP1 (Figure 4B).
Gene_expression (overexpression) of OSBP1
9) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
We found that overexpression of OSBP1 in cells stably expressing APP led to downregulation of the amyloidogenic processing shown by a decrease in APP-CTF?
Gene_expression (overexpression) of OSBP1
10) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0.14 Pain Relevance 0
It is of interest that while OSBP1 overexpression produced no obvious changes in the localization of APP, an altered localization of APP-Notch2 dimers was detected.
Gene_expression (overexpression) of OSBP1
11) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0.06 Pain Relevance 0
OSBP1 overexpression did not appear to produce changes in APP subcellular localization.
Gene_expression (overexpression) of OSBP1
12) Confidence 0.76 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0.07 Pain Relevance 0
42 levels were significantly reduced in HEK-APPNEFV cells expressing OSBP1 (Figure 2B).
Gene_expression (expressing) of OSBP1
13) Confidence 0.66 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
Similar to the findings in H4-APP cells, OSBP1 overexpression in HEK-APPNFEV cells did not produce significant changes in the steady-state levels of total APP nor led to changes in cell surface distribution of APP as assessed by cell surface biotinylation experiments (Figure 2C).
Gene_expression (overexpression) of OSBP1
14) Confidence 0.66 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
For overexpression experiments, the human OSBP1 cDNA was transfected into HEK-APPNFEV cells (70–80% confluence) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions.
Gene_expression (overexpression) of OSBP1
15) Confidence 0.66 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
H4 cells were transfected with APPYC plus N2C50YN in the absence or presence of overexpressed OSBP1 and then treated with 1 ?
Gene_expression (overexpressed) of OSBP1
16) Confidence 0.66 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
Using BiFC we demonstrated that OSBP1 appeared to modulate the distribution of APP-Notch2 heterodimers (Figure 5A).
Gene_expression (appeared) of OSBP1
17) Confidence 0.66 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
in the media measured by ELISA in HEK-APPNFEV were also unexpectedly reduced by 24% in HEK-APPNEFV overexpressing OSBP1 (data not shown), suggesting again that OSBP1 overexpression impacts the non-amyloidogenic processing of APP in a more complex manner.


Gene_expression (overexpressing) of OSBP1
18) Confidence 0.59 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
Interestingly, we found that OSBP1 overexpression did not alter constitutive sAPP?
Gene_expression (overexpression) of OSBP1
19) Confidence 0.59 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
Since no apparent changes in cell surface distribution of APP were detected (Figure 2C), we investigated the potential effects of OSBP1 overexpression on the subcellular localization of APP.
Gene_expression (overexpression) of OSBP1
20) Confidence 0.59 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0

General Comments

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