INT230174

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.68
First Reported 2008
Last Reported 2008
Negated 1
Speculated 0
Reported most in Body
Documents 1
Total Number 20
Disease Relevance 0.75
Pain Relevance 0

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

lipid binding (OSBP) transport (OSBP) Golgi apparatus (OSBP)
cytoplasm (OSBP)
Anatomy Link Frequency
plasma 2
OSBP (Homo sapiens)
Pain Link Frequency Relevance Heat
agonist 20 46.92 Quite Low
cINOD 60 5.00 Very Low Very Low Very Low
Kinase C 20 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Alzheimer's Dementia 100 78.24 Quite High
Adhesions 20 73.44 Quite High
Disease 180 71.84 Quite High
Stress 20 5.00 Very Low Very Low Very Low
INFLAMMATION 20 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
It is of interest that while OSBP1 overexpression produced no obvious changes in the localization of APP, an altered localization of APP-Notch2 dimers was detected.
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
1) Confidence 0.68 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0.06 Pain Relevance 0
When OSBP1 was not overexpressed, 25OH had no effect on the localization of the APP-Notch2 dimers, perhaps because basal levels of OSBP1 were not high enough to counteract the effects of 25OH binding to other ORPs or LXRs.
Neg (not) Positive_regulation (overexpressed) of Neg (not) Gene_expression (overexpressed) of OSBP1
2) Confidence 0.68 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0.06 Pain Relevance 0
However, by using bimolecular fluorescence complementation to study the movement of APP dimers, we found that OSBP1 overexpression caused sequestration of APP-Notch2 dimers in the Golgi.
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
3) Confidence 0.68 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0.07 Pain Relevance 0
We found that overexpression of OSBP1 in cells stably expressing APP led to downregulation of the amyloidogenic processing shown by a decrease in APP-CTF?
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
4) Confidence 0.68 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0.14 Pain Relevance 0
The effect of OSBP1 overexpression on A?
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
5) Confidence 0.68 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
H4 cells were transfected with APPYC plus N2C50YN in the absence or presence of overexpressed OSBP1 and then treated with 1 ?
Positive_regulation (overexpressed) of Gene_expression (overexpressed) of OSBP1
6) Confidence 0.68 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
In addition, OSBP1 overexpression was unable to further alter the concomitant increase in APP-CTF?
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
7) Confidence 0.68 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
OSBP1 overexpression did not appear to produce changes in APP subcellular localization.
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
8) Confidence 0.68 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0.07 Pain Relevance 0
APP was found throughout fractions 4–12, and this distribution was not altered by overexpression of OSBP1 (Figure 4B).
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
9) Confidence 0.68 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
H4 cells were transfected with APPYC plus N2C50YN in the absence or presence of overexpressed OSBP1 and then treated with 1 ?
Positive_regulation (overexpressed) of Gene_expression (overexpressed) of OSBP1
10) Confidence 0.49 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
For overexpression experiments, the human OSBP1 cDNA was transfected into HEK-APPNFEV cells (70–80% confluence) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions.
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
11) Confidence 0.49 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
We also performed sucrose gradient fractionation of APP-overexpressing cells transiently transfected with OSBP1 or empty vector (Figure 4B and 4C).
Positive_regulation (transfected) of Gene_expression (transfected) of OSBP1
12) Confidence 0.49 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
Similar to the findings in H4-APP cells, OSBP1 overexpression in HEK-APPNFEV cells did not produce significant changes in the steady-state levels of total APP nor led to changes in cell surface distribution of APP as assessed by cell surface biotinylation experiments (Figure 2C).
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
13) Confidence 0.49 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
We investigated whether OSBP1 was involved in regulating APP processing and found that overexpression of OSBP1 downregulated the amyloidogenic processing of APP, while OSBP1 knockdown had the opposite effect.
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
14) Confidence 0.45 Published 2008 Journal Mol Neurodegener Section Abstract Doc Link PMC2323375 Disease Relevance 0.37 Pain Relevance 0
were observed with OSBP1 overexpression in H4-APP cells (Figure 2A).
Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
15) Confidence 0.45 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
Overexpression of OSBP1 inhibited APP processing and sAPP secretion
Positive_regulation (Overexpression) of Gene_expression (Overexpression) of OSBP1
16) Confidence 0.45 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
in the media measured by ELISA in HEK-APPNFEV were also unexpectedly reduced by 24% in HEK-APPNEFV overexpressing OSBP1 (data not shown), suggesting again that OSBP1 overexpression impacts the non-amyloidogenic processing of APP in a more complex manner.


Positive_regulation (overexpression) of Gene_expression (overexpression) of OSBP1
17) Confidence 0.45 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
Briefly, HEK-APPNFEV cells were plated in 10 cm dishes and transfected with OSBP1 cDNA as described above. 48 h following transfection, cell monolayers were quickly rinsed twice with ice-cold PBS and biotinylation of cell surface proteins was carried our according to manufacturer instructions.
Positive_regulation (transfected) of Gene_expression (transfected) of OSBP1
18) Confidence 0.45 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
in the media measured by ELISA in HEK-APPNFEV were also unexpectedly reduced by 24% in HEK-APPNEFV overexpressing OSBP1 (data not shown), suggesting again that OSBP1 overexpression impacts the non-amyloidogenic processing of APP in a more complex manner.


Positive_regulation (overexpressing) of Gene_expression (overexpressing) of OSBP1
19) Confidence 0.45 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0
Treatment of cells overexpressing OSBP1 with 25OH shifted the distribution of APP-N2C50 hetero-dimers to a distribution consistent with ER and plasma membrane localization (Figure 5B, panel c), whereas treatment with 25OH had no effect on heterodimer localization in cells not overexpressing OSBP1 (Figure 5B, panel d).
Positive_regulation (overexpressing) of Gene_expression (overexpressing) of OSBP1 in plasma
20) Confidence 0.40 Published 2008 Journal Mol Neurodegener Section Body Doc Link PMC2323375 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox