INT232848

Context Info Confidence 0.36 First Reported 2008 Last Reported 2008 Negated 0 Speculated 0 Reported most in Body Documents 5 Total Number 5 Disease Relevance 2.30 Pain Relevance 0.08

structural molecule activity (Krt18) microtubule organizing center (Krt18) cytoplasm (Krt18)

Krt18 (Mus musculus)

Pain Link Frequency Relevance Heat Bile 12 79.60 alcohol 12 43.00 fibrosis 32 5.00 Paracetamol 16 5.00 Inflammation 12 5.00 Potency 4 5.00 imagery 4 5.00 palliative 1 5.00

Disease Link Frequency Relevance Heat Targeted Disruption 56 100.00 Ovarian Cancer 41 99.74 Aging 8 96.28 Stress 72 94.96 Cancer 73 93.56 Solid Tumor 16 85.28 Nash(non-alcoholic Steatohepatitis) 28 81.76 Parkinson's Disease 4 80.72 Pressure And Volume Under Development 24 76.88 Hepatotoxicity 8 74.68

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

In order to determine if our cell lines presented these EOC markers, we monitored protein expression of Krt7, Krt8 and Krt18 by western blot.

Negative_regulation (monitored) of Gene_expression (expression) of Krt18 associated with ovarian cancer

1) Confidence 0.36 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2467432 Disease Relevance 0.79 Pain Relevance 0

Dysbalanced K8/K18 expression precedes MDB formation, likely increases keratin misfolding and predisposes to posttranslational modifications, which may interfere with keratin refolding and/or repair.

Negative_regulation (Dysbalanced) of Gene_expression (expression) of K18

2) Confidence 0.35 Published 2008 Journal Histochem Cell Biol Section Body Doc Link PMC2386529 Disease Relevance 0.30 Pain Relevance 0

Dysbalanced K8/K18 expression precedes MDB formation, likely increases keratin misfolding and predisposes to posttranslational modifications, which may interfere with keratin refolding and/or repair.

Negative_regulation (precedes) of Gene_expression (expression) of K18

3) Confidence 0.35 Published 2008 Journal Histochem Cell Biol Section Body Doc Link PMC2386529 Disease Relevance 0.30 Pain Relevance 0

Several pathogenic mechanisms were implicated in this process (Fig. 4; Dobson 2004):1.Enhanced oxidative stress2.Disproportional K8/K18 expression together with keratin modifications3.Chaperone dysfunction4.Elevated p62 levels5.Insufficient protein degradation

Negative_regulation (stress2.Disproportional) of Gene_expression (expression) of K18

4) Confidence 0.35 Published 2008 Journal Histochem Cell Biol Section Body Doc Link PMC2386529 Disease Relevance 0.22 Pain Relevance 0.08

The elevated K8/K18 ratio is crucial for MDB formation as shown in K18-knockout and K8 overexpressing animals, who are predisposed to MDB formation already upon short exposure to DDC and even form MDBs spontaneously during aging (Magin et al. 1998; Nakamichi et al. 2005).

Negative_regulation (predisposed) of Gene_expression (overexpressing) of K18-knockout associated with targeted disruption and aging

5) Confidence 0.30 Published 2008 Journal Histochem Cell Biol Section Body Doc Link PMC2386529 Disease Relevance 0.70 Pain Relevance 0

General Comments

This test has worked.