INT234081

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Context Info
Confidence 0.51
First Reported 2008
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 9
Total Number 10
Disease Relevance 0.71
Pain Relevance 0.94

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

vesicle-mediated transport (Vamp2) plasma membrane (Vamp2) protein complex assembly (Vamp2)
Anatomy Link Frequency
spinal cord neurons 2
synaptic vesicles 1
vesicle 1
melanotrophs 1
Vamp2 (Mus musculus)
Pain Link Frequency Relevance Heat
Spinal cord 94 99.84 Very High Very High Very High
tetrodotoxin 32 94.48 High High
Action potential 17 69.04 Quite High
Hippocampus 37 52.00 Quite High
Neurotransmitter 102 50.00 Quite Low
Inflammation 6 43.60 Quite Low
long-term potentiation 6 12.00 Low Low
Pyramidal cell 24 5.00 Very Low Very Low Very Low
withdrawal 20 5.00 Very Low Very Low Very Low
Glutamate 20 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Disease 60 95.20 Very High Very High Very High
Wallerian Degeneration 30 85.68 High High
Disease Progression 6 78.76 Quite High
Stress 6 70.32 Quite High
Neurodegenerative Disease 30 67.92 Quite High
Creutzfeldt Jakob Disease 24 67.40 Quite High
Lifespan 2 55.68 Quite High
Anaerobic Bacterial Infections 44 50.00 Quite Low
Schizophrenia 21 50.00 Quite Low
INFLAMMATION 6 43.60 Quite Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Sphingosine was dialyzed into the cytosol of cultured rat pituitary intermediate lobe cells (melanotrophs), which express synaptobrevin-2 (Jacobsson and Meister, 1996).
Gene_expression (express) of synaptobrevin-2 in melanotrophs
1) Confidence 0.51 Published 2009 Journal Neuron Section Body Doc Link PMC2697323 Disease Relevance 0 Pain Relevance 0
GST–syntaxin-1, GST–SNAP-25, and GS–VAMP-2 were expressed in E. coli and purified with glutathione–Sepharose 4B (GE Healthcare) in accordance with the manufacturer's manual.
Gene_expression (expressed) of VAMP-2
2) Confidence 0.37 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2568027 Disease Relevance 0 Pain Relevance 0
For the expression of GST-fused syntaxin-1 (GST–syntaxin-1), GST-fused SNAP-25 (GST–SNAP-25), and GST-fused VAMP-2 (GST–VAMP-2), cDNAs encoding the cytoplasmic domain of syntaxin-1 (1–265 aa), full-length SNAP-25 (1–206 aa), and the cytoplasmic domain of VAMP-2 (1–94 aa) were subcloned into pGEX vectors (GE Healthcare).
Gene_expression (expression) of VAMP-2
3) Confidence 0.37 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2568027 Disease Relevance 0 Pain Relevance 0
-synuclein in hippocampal neurons has a profound effect in down-regulating the expression of a number of important proteins in synaptic vesicles including VAMP-2, piccolo and synpasin-1 (Scott et al., 2010).
Gene_expression (expression) of VAMP-2 in synaptic vesicles
4) Confidence 0.29 Published 2010 Journal ASN NEURO Section Body Doc Link PMC2954441 Disease Relevance 0.43 Pain Relevance 0.03
First, we examined the effect of tomosyn deficiency on the expression levels of SNARE proteins, including syntaxin-1, SNAP-25, VAMP-2, and complexin.
Gene_expression (expression) of VAMP-2
5) Confidence 0.28 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2568027 Disease Relevance 0 Pain Relevance 0
For the expression of GST-fused syntaxin-1 (GST–syntaxin-1), GST-fused SNAP-25 (GST–SNAP-25), and GST-fused VAMP-2 (GST–VAMP-2), cDNAs encoding the cytoplasmic domain of syntaxin-1 (1–265 aa), full-length SNAP-25 (1–206 aa), and the cytoplasmic domain of VAMP-2 (1–94 aa) were subcloned into pGEX vectors (GE Healthcare).
Gene_expression (expression) of VAMP-2
6) Confidence 0.28 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2568027 Disease Relevance 0 Pain Relevance 0
To test the physiological relevance of these observations, we monitored syb II levels in spinal cord neurons (Figure 2C) and observed that under high potassium conditions syb II immunofluorescence was reduced by 50% as compared to control and non-stimulating (i.e.
Gene_expression (levels) of syb II in spinal cord neurons associated with spinal cord
7) Confidence 0.23 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2991259 Disease Relevance 0 Pain Relevance 0.46
A number of proteins associated with the synaptic vesicle membrane were the first to show reduced levels of expression, including VAMP-2 (vesicle-associated membrane protein-2), synaptophysin and the chaperone CSP (cysteine string protein) (Gray et al., 2009), and these proteins were also reduced when compared with the levels of PSD-related proteins.
Gene_expression (expression) of vesicle-associated membrane protein-2 in vesicle
8) Confidence 0.22 Published 2010 Journal ASN NEURO Section Body Doc Link PMC2954441 Disease Relevance 0.28 Pain Relevance 0
In our experiments, we did not find any changes in the steady-state levels of these exocytotic regulators, including SNARE proteins (SNAP25, syntaxin-1, and VAMP2), synaptotagamin-1, VGLUT1, munc18-1, dynamin-1, and complexin1/2 (Fig. 7).
Gene_expression (/) of VAMP2
9) Confidence 0.22 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2396815 Disease Relevance 0 Pain Relevance 0
To test the physiological relevance of these observations, we monitored syb II levels in spinal cord neurons (Figure 2C) and observed that under high potassium conditions syb II immunofluorescence was reduced by 50% as compared to control and non-stimulating (i.e.
Gene_expression (immunofluorescence) of syb II in spinal cord neurons associated with spinal cord
10) Confidence 0.20 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2991259 Disease Relevance 0 Pain Relevance 0.45

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