INT234825

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Context Info
Confidence 0.59
First Reported 2008
Last Reported 2010
Negated 1
Speculated 0
Reported most in Body
Documents 16
Total Number 17
Disease Relevance 1.15
Pain Relevance 2.71

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (RAB5A) plasma membrane (RAB5A) GTPase activity (RAB5A)
Anatomy Link Frequency
upper 6
macrophages 2
RAB5A (Homo sapiens)
Pain Link Frequency Relevance Heat
agonist 1484 99.78 Very High Very High Very High
Dopamine 14 99.32 Very High Very High Very High
cytokine 1 93.12 High High
imagery 102 58.24 Quite High
dopamine receptor 14 5.00 Very Low Very Low Very Low
cINOD 14 5.00 Very Low Very Low Very Low
ischemia 14 5.00 Very Low Very Low Very Low
Pain 3 5.00 Very Low Very Low Very Low
cva 2 5.00 Very Low Very Low Very Low
headache 2 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Infection 168 94.12 High High
Salmonella Infection 1 70.56 Quite High
Viral Infection 1 70.40 Quite High
Death 3 57.76 Quite High
Dengue 22 50.00 Quite Low
Hemophagocytic Lymphohistiocytosis 2 46.96 Quite Low
Syndrome 21 43.96 Quite Low
General Immunology 1 42.44 Quite Low
Congenital Anomalies 3 37.12 Quite Low
Toxicity 2 22.88 Low Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Failure to detect corresponding agonist-dependent increases in Rab5 expression in the P100 fractions owing to its translocation from the S100 fractions, except at the 2 h time point, was most likely simply due to the saturation of the chemiluminescence detection system owing to the already high levels of Rab5 associated with the P100 fraction, even in the absence of agonist.
Positive_regulation (increases) of Gene_expression (expression) of Rab5 associated with agonist
1) Confidence 0.59 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.29
Whilst there was no change in the overall level of Rab5 protein expression over the duration of cicaprost stimulation, there was a significant decrease in the levels of Rab5 in the S100 fraction as early as 30 min, which continued to decrease to barely detectable levels at 2–3 h post agonist stimulation (Figs. 4A and B).
Positive_regulation (level) of Gene_expression (expression) of Rab5 associated with agonist
2) Confidence 0.43 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.21
Hence, unlike that of the hIP, over-expression of Rab5a did not specifically augment or alter the trafficking by the hIP?
Neg (not) Positive_regulation (augment) of Gene_expression (expression) of Rab5a
3) Confidence 0.43 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.10
In the case of the dopamine D2 receptor, internalization was accelerated by over-expression of Rab5a and Rab5aQ79L and inhibited by Rab5aS34N [35]. ?
Positive_regulation (accelerated) of Gene_expression (expression) of Rab5a associated with dopamine
4) Confidence 0.43 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.16
The increase in immunoprecipitation of Rab5a in response to agonist was not due to an increase in hIP or Rab5a expression levels (data not shown and Fig. 5; lower panel) or in the level of the hIP present in the anti-HA immunoprecipitates per se (Fig. 5; middle panel) and, hence, was due to enhanced interaction between hIP and Rab5a.
Positive_regulation (increase) of Gene_expression (expression) of Rab5a associated with agonist
5) Confidence 0.43 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.25
The expression of significant levels of both endogenous Rab5 and increased expression of recombinant Rab5a in HEK.hIP cells (Fig. 1D) and indeed in the parental HEK 293 cell line (data not shown) was initially confirmed by western blot analysis.
Positive_regulation (increased) of Gene_expression (expression) of Rab5a
6) Confidence 0.43 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.14
The expression of significant levels of both endogenous Rab5 and increased expression of recombinant Rab5a in HEK.hIP cells (Fig. 1D) and indeed in the parental HEK 293 cell line (data not shown) was initially confirmed by western blot analysis.
Positive_regulation (increased) of Gene_expression (expression) of Rab5
7) Confidence 0.43 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.15
Taken together, data herein have established that the hIP undergoes significant time-dependent internalization in response to cicaprost stimulation; over-expression of Rab5a increases that internalization; the internalized hIP co-localizes to Rab5a positive endosomes and agonist stimulation leads to direct translocation-dependent activation of Rab5.
Positive_regulation (increases) of Gene_expression (expression) of Rab5a associated with agonist
8) Confidence 0.43 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.17
In the case of the dopamine D2 receptor, internalization was accelerated by over-expression of Rab5a and Rab5aQ79L and inhibited by Rab5aS34N [35]. ?
Positive_regulation (accelerated) of Gene_expression (expression) of Rab5aQ79L associated with dopamine
9) Confidence 0.43 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.16
Through ELISA-based assays, it was established that over-expression of Rab5a led to a significant increase in cicaprost-induced internalization throughout the initial 3 h duration post-agonist stimulation but did not interfere with the level of the hIP recycled at the 4 h time point.
Positive_regulation (expression) of Gene_expression (expression) of Rab5a associated with agonist
10) Confidence 0.40 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.22
The extent of association between the hIP and Rab5aS34N was similar to that of wild type Rab5a (Fig. 5; upper panel) as were the amount of hIP present in the immunoprecipitates and the levels of GFP-Rab5a and -Rab5aS34N over-expression in the HEK.hIP cells (Fig. 5, middle and lower panels, respectively).
Positive_regulation (over) of Gene_expression (-) of Rab5aS34N in upper
11) Confidence 0.40 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.20
The extent of association between the hIP and Rab5aS34N was similar to that of wild type Rab5a (Fig. 5; upper panel) as were the amount of hIP present in the immunoprecipitates and the levels of GFP-Rab5a and -Rab5aS34N over-expression in the HEK.hIP cells (Fig. 5, middle and lower panels, respectively).
Positive_regulation (over) of Gene_expression (over) of Rab5aS34N in upper
12) Confidence 0.40 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.20
Through ELISA-based assays, it was established that over-expression of Rab5a led to a significant increase in cicaprost-induced internalization throughout the initial 3 h duration post-agonist stimulation but did not interfere with the level of the hIP recycled at the 4 h time point.
Positive_regulation (over) of Gene_expression (expression) of Rab5a associated with agonist
13) Confidence 0.40 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.22
The extent of association between the hIP and Rab5aS34N was similar to that of wild type Rab5a (Fig. 5; upper panel) as were the amount of hIP present in the immunoprecipitates and the levels of GFP-Rab5a and -Rab5aS34N over-expression in the HEK.hIP cells (Fig. 5, middle and lower panels, respectively).
Positive_regulation (over) of Gene_expression (expression) of GFP-Rab5a in upper
14) Confidence 0.35 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.20
In fact, this observation was consistent with the previous reports that the over-expression of Rab5 can alter endocytosis by increasing receptor (with attached virus) recycling back to the cell surface [41].
Positive_regulation (over) of Gene_expression (over) of Rab5
15) Confidence 0.31 Published 2010 Journal Virol J Section Body Doc Link PMC2825209 Disease Relevance 0.48 Pain Relevance 0
In fact, this observation was consistent with the previous reports that the over-expression of Rab5 can alter endocytosis by increasing receptor (with attached virus) recycling back to the cell surface [41].
Positive_regulation (over) of Gene_expression (expression) of Rab5
16) Confidence 0.31 Published 2010 Journal Virol J Section Body Doc Link PMC2825209 Disease Relevance 0.48 Pain Relevance 0
In L. monocytogenes-infected macrophages, overexpression of Rab5a stimulates the intracellular degradation of the pathogen [6].
Positive_regulation (overexpression) of Gene_expression (overexpression) of Rab5a in macrophages
17) Confidence 0.19 Published 2008 Journal Eur J Pediatr Section Body Doc Link PMC2413085 Disease Relevance 0.20 Pain Relevance 0.05

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