INT234952
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Proteolysis of Dsg1 resulted in a rapid degradation of recombinant Dsg1 into fragments undetectable by western blot. | |||||||||||||||
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Proteolysis of Dsg1 resulted in a rapid degradation of recombinant Dsg1 into fragments undetectable by western blot. | |||||||||||||||
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Recombinant Dsg2 was also cleaved by hK7 in a time-dependent manner, however, in contrast to the complete proteolysis of Dsg1, the cleavage of Dsg2 resulted in distinct proteolytic fragments (Fig. 3B, left). | |||||||||||||||
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In support of an association between hK7 and the desmogleins, hK7 was able to proteolyze both Dsg1 and Dsg2. | |||||||||||||||
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Upon incubation with thermolysin-activated hK7, recombinant Dsg1 was rapidly degraded with little detectable protein remaining after 60 minutes (Fig. 3A, left). | |||||||||||||||
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The ability of hK7 to degrade Dsg1 and Dsg2 was investigated using in vitro degradation assays. | |||||||||||||||
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hK7 cleaves both recombinant Dsg1 and Dsg2 in vitro | |||||||||||||||
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Nevertheless, the fact that specific cleavage of Dsg 1 by staphylococcal exfoliative toxin in bullous impetigo is sufficient to cause a histologic phenotype comparable to PF (Amagai et al. 2000a; Hanakawa et al. 2002) indicates that, in principle, specific proteolysis could be an effective mechanism in pemphigus.
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Most cases are caused by staphylococcal exfoliative toxin (ET), a serine protease, which has been shown to selectively cleave Dsg 1 between EC 3 and 4 in conformation-dependent manner, but not Dsg 3 or E-cadherin (TableĀ 1) (Amagai et al. 2000a, 2002; Hanakawa et al. 2002; Melish and Glasgow 1970). | |||||||||||||||
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General Comments
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