INT235381

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Context Info
Confidence 0.02
First Reported 2008
Last Reported 2008
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 18
Disease Relevance 5.25
Pain Relevance 0.10

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (MECOM) nucleus (MECOM) intracellular (MECOM)
DNA binding (MECOM)
Anatomy Link Frequency
nerve 2
band 2
plasma 1
juvenile 1
A band 1
MECOM (Homo sapiens)
Pain Link Frequency Relevance Heat
isoflurane 18 72.00 Quite High
anesthesia 36 61.28 Quite High
cocaine 18 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Targeted Disruption 954 99.80 Very High Very High Very High
Cytomegalovirus Infection 198 95.36 Very High Very High Very High
Myelodysplastic Syndromes 18 69.72 Quite High
Sprains And Strains 54 63.60 Quite High
Respiratory Disease 18 47.40 Quite Low
Congenital Anomalies 18 45.60 Quite Low
Poisoning 36 27.24 Quite Low
Coagulation Disorder 18 17.56 Low Low
Toxicity 36 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Hollow fiber production of stable transfectants was established to produce the fusion protein.


Gene_expression (produce) of fusion protein
1) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.49 Pain Relevance 0
The purified fusion protein produced in vitro was injected intravenously into juvenile pigs for pharmacokinetic study.
Gene_expression (produced) of fusion protein in juvenile
2) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Abstract Doc Link PMC2415180 Disease Relevance 0.15 Pain Relevance 0
The production of the fusion protein in the milk of transgenic goats provided further evidence that sufficient quantities of BChE/hSA can be produced to serve as a cost-effective and reliable source of BChE for prophylaxis and post-exposure treatment.



Gene_expression (production) of fusion protein associated with targeted disruption
3) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Abstract Doc Link PMC2415180 Disease Relevance 0.24 Pain Relevance 0
Production of the fusion protein from a selected clone, clone 35, using a hollow fiber system after transfection with the in vitro DNA expression vector, CMV/BChE/hSA, in BHK cells was initiated to generate sufficient material (~1 g in ~30 liters of media, 30 mg/liter) for purification with ammonium sulfate precipitation, followed by procainamide affinity chromatography.
Gene_expression (Production) of fusion protein associated with cytomegalovirus infection
4) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.25 Pain Relevance 0
An activity gel staining using conditioned media from BHK cells expressing the fusion protein demonstrated the secretion of an active enzyme, with the major band migrating faster than the plasma huBChE tetramer (Fig. 1A lane 2).
Gene_expression (expressing) of fusion protein in band
5) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.14 Pain Relevance 0
A minor band appeared at ~90 kDa in the lanes, suggesting proteolytic products of the fusion protein.


Gene_expression (products) of fusion protein in band
6) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.22 Pain Relevance 0
The production of the fusion protein in the milk of transgenic goats provided further evidence that it is possible to produce such a protein in large scale and in a cost-effective way.
Gene_expression (production) of fusion protein associated with targeted disruption
7) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.64 Pain Relevance 0
Purified BChE/hSA fusion protein produced in vitro was injected i.v. into 2 juvenile pigs of ~20 kg (10 mg/kg, using a converting equation of 720 units = 1 mg purified plasma huBChE [29]).
Gene_expression (produced) of fusion protein in plasma
8) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.06 Pain Relevance 0.10
The fusion protein was produced first in vitro in BHK cells and used in a pharmacokinetic study as a proof-of-concept experiment, followed by fast generation of transgenic mice.
Gene_expression (produced) of fusion protein associated with targeted disruption
9) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.32 Pain Relevance 0
Secretion level of the fusion protein produced in vitro in BHK cells was ~30 mg/liter.
Gene_expression (produced) of fusion protein
10) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Abstract Doc Link PMC2415180 Disease Relevance 0.10 Pain Relevance 0
Identification of transgenic mice and expression of the fusion protein in the milk
Gene_expression (expression) of fusion protein associated with targeted disruption
11) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.32 Pain Relevance 0
There was a few years' delay from producing the fusion protein from BHK cells to obtaining the protein from transgenic goats.
Gene_expression (producing) of fusion protein associated with targeted disruption
12) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.58 Pain Relevance 0
Both the pharmacokinetic study and the in vitro nerve agent binding and inhibition assay suggested that a fusion protein retaining both properties of huBChE and hSA is produced in vitro and in vivo.
Gene_expression (produced) of fusion protein in nerve
13) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Abstract Doc Link PMC2415180 Disease Relevance 0.24 Pain Relevance 0
Western blot analysis under denaturing and reducing conditions confirmed the expression of the fusion protein in BHK cells with expected molecular mass of ~150 kDa (Fig. 1B lane 3).
Gene_expression (expression) of fusion protein
14) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.07 Pain Relevance 0
A band appeared at ~90 kDa in the lane and it may belong to proteolytic products of the fusion protein.
Gene_expression (products) of fusion protein in A band
15) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.07 Pain Relevance 0
It is possible to produce such a fusion protein in large scale and in a cost-effective way in the milk of transgenic goats.


Gene_expression (produce) of fusion protein associated with targeted disruption
16) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.48 Pain Relevance 0
Further, transgenic goats were generated for large-scale production of the fusion protein.


Gene_expression (production) of fusion protein associated with targeted disruption
17) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.38 Pain Relevance 0
The pharmacokinetic study and the in vitro nerve agent binding and inhibition assay suggested that a huBChE/hSA fusion protein retaining both properties of huBChE and hSA is produced in vitro in BHK cells and in vivo in the milk of transgenic animals.
Gene_expression (produced) of fusion protein in nerve associated with targeted disruption
18) Confidence 0.02 Published 2008 Journal BMC Biotechnol Section Body Doc Link PMC2415180 Disease Relevance 0.49 Pain Relevance 0

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