INT237038

Context Info Confidence 0.36 First Reported 2008 Last Reported 2010 Negated 0 Speculated 1 Reported most in Body Documents 18 Total Number 21 Disease Relevance 13.68 Pain Relevance 0.31

Golgi apparatus (Becn1) cytoplasmic membrane-bounded vesicle (Becn1) cytoskeleton (Becn1) cell cycle (Becn1) cell division (Becn1) protein complex (Becn1)

Anatomy Link Frequency neuronal 2 brains 1 groove 1 band 1 Becn1 (Mus musculus)

Pain Link Frequency Relevance Heat Inflammatory response 5 92.88 Inflammation 20 90.64 Pyramidal cell 12 62.40 chemokine 5 59.04 depression 6 28.72 Clonidine 32 5.00 anesthesia 13 5.00 Central nervous system 11 5.00 Hippocampus 10 5.00 halothane 7 5.00

Disease Link Frequency Relevance Heat Stress 167 100.00 Infection 457 99.84 Alzheimer's Dementia 74 99.84 Sprains And Strains 37 99.84 Targeted Disruption 77 99.72 Apoptosis 770 99.68 Parkinson's Disease 154 99.08 Togavirus Infection 82 99.08 Urological Neuroanatomy 2 98.60 Disease 389 97.32

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These include Ifi27, Ifih1, Irf7 and Oas1b which were upregulated at 72 hr and 96 hr post infection except Becn1 that was over-expressed only at 72 hr post infection, and Spn which was over-expressed at all time points.

Gene_expression (expressed) of Becn1 associated with urological neuroanatomy and infection

1) Confidence 0.36 Published 2008 Journal BMC Genomics Section Body Doc Link PMC2440554 Disease Relevance 1.43 Pain Relevance 0.23

Levels of other components of the autophagy pathway such as Atg5, Atg10, Atg12 and Beclin-1 were not different in DLB compared to controls.

Gene_expression (Levels) of Beclin-1 associated with parkinson's disease

2) Confidence 0.28 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2824828 Disease Relevance 1.56 Pain Relevance 0

Beclin-1 was detected as a single band at 50 kDa that was more abundant in the membrane than the cytosolic fraction (Figure 1A, B).

Gene_expression (detected) of Beclin-1 in band

3) Confidence 0.28 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2824828 Disease Relevance 0.92 Pain Relevance 0.03

The elevated expression of Beclin-1 and LC3-II following lethal toxin exposure was further substantiated by the overtly increased GFP-LC3 puncta staining in H9C2 cells following lethal toxin challenge.

Gene_expression (expression) of Beclin-1

4) Confidence 0.15 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2954163 Disease Relevance 0.64 Pain Relevance 0.03

Assessment of ER stress and autophagy revealed a significant increase in Bip, Beclin-1 and LC3-II expression following lethal toxin exposure, suggesting possible involvements of ER stress and autophagy.

Gene_expression (expression) of Beclin-1 associated with stress

5) Confidence 0.15 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2954163 Disease Relevance 0.59 Pain Relevance 0.03

Conversely, increasing Beclin 1 expression results in diminished amyloid pathology in these AD transgenic mice [57].

Gene_expression (expression) of Beclin 1 associated with targeted disruption and alzheimer's dementia

6) Confidence 0.14 Published 2010 Journal Seminars in Cell & Developmental Biology Section Body Doc Link PMC2938570 Disease Relevance 1.58 Pain Relevance 0

One probable contributor to autophagy deficiency in AD appears to be Beclin 1, whose expression is strongly reduced in the brains of AD patients to levels that would be predicted to impair autophagosome synthesis [56].

Gene_expression (expression) of Beclin 1 in brains associated with alzheimer's dementia

7) Confidence 0.12 Published 2010 Journal Seminars in Cell & Developmental Biology Section Body Doc Link PMC2938570 Disease Relevance 1.47 Pain Relevance 0

Genetic manipulations that decrease Beclin 1 levels in AD transgenic mice reduce neuronal autophagy, disrupt lysosomes, promote intracellular and extracellular A?

Gene_expression (levels) of Beclin 1 in neuronal associated with targeted disruption and alzheimer's dementia

8) Confidence 0.12 Published 2010 Journal Seminars in Cell & Developmental Biology Section Body Doc Link PMC2938570 Disease Relevance 1.58 Pain Relevance 0

Recently, delivery of the Beclin 1 gene was shown to induce autophagy and reduce amyloid and ?

Gene_expression (delivery) of Beclin 1 gene associated with alzheimer's dementia

9) Confidence 0.12 Published 2010 Journal Seminars in Cell & Developmental Biology Section Body Doc Link PMC2938570 Disease Relevance 0.94 Pain Relevance 0

Our results revealed that lethal toxin produced subtle although significant upregulation in the expression of the ER stress maker BIP and the autophagy markers Beclin-1 and LC3-II without affecting the phospho-eIF2?

Gene_expression (expression) of Beclin-1 associated with stress

10) Confidence 0.11 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2954163 Disease Relevance 0.57 Pain Relevance 0

Neuronal overexpression of the autophagy protein Beclin1 confers resistance to Sindbis virus infections [7].

Gene_expression (overexpression) of Beclin1 in Neuronal associated with togavirus infection and infection

11) Confidence 0.07 Published 2009 Journal PLoS Pathogens Section Body Doc Link PMC2752191 Disease Relevance 0.72 Pain Relevance 0

BH2 mutant defective for apoptosis inhibition yet retaining Beclin1-binding and autophagy inhibition intact maintained viral genome loads equivalent to WT virus (Figure 7D, 7E, 7F down and S5D), arguing that the ?

Gene_expression (retaining) of Beclin1-binding associated with apoptosis

12) Confidence 0.06 Published 2009 Journal PLoS Pathogens Section Body Doc Link PMC2752191 Disease Relevance 0.46 Pain Relevance 0

These data collectively demonstrate that the BH2 domain of the hydrophobic groove of vBcl-2 is not essential for suppressing Beclin1-mediated autophagy and its elimination does not affect Beclin1 binding, whereas the elimination of ?

Gene_expression (suppressing) of Beclin1 in groove

13) Confidence 0.06 Published 2009 Journal PLoS Pathogens Section Body Doc Link PMC2752191 Disease Relevance 0 Pain Relevance 0

In contrast, 3T3 cells infected with the Beclin1-binding-deficient vBcl-2 mutant viruses (?

Gene_expression (infected) of Beclin1-binding

14) Confidence 0.06 Published 2009 Journal PLoS Pathogens Section Body Doc Link PMC2752191 Disease Relevance 0.08 Pain Relevance 0

To analyze the interactions between the Beclin1 and vBcl-2 mutants, yeast strain AH109, expressing the BH3-like domain of Beclin1 fused to the Gal4 activation domain in the pGADT7 plasmid, was used to transform pGBKT7 plasmids containing the mutants of vBcl-2, and the transformants then assayed for ?

Spec (analyze) Gene_expression (mutants) of Beclin1 associated with sprains and strains

15) Confidence 0.06 Published 2009 Journal PLoS Pathogens Section Body Doc Link PMC2752191 Disease Relevance 0.30 Pain Relevance 0

To analyze the interactions between the Beclin1 and vBcl-2 mutants, yeast strain AH109, expressing the BH3-like domain of Beclin1 fused to the Gal4 activation domain in the pGADT7 plasmid, was used to transform pGBKT7 plasmids containing the mutants of vBcl-2, and the transformants then assayed for ?

Gene_expression (expressing) of Beclin1 associated with sprains and strains

16) Confidence 0.06 Published 2009 Journal PLoS Pathogens Section Body Doc Link PMC2752191 Disease Relevance 0.31 Pain Relevance 0

Similar results were also observed with endogenous Beclin1 in 293T cells, in that removal of the ?

Gene_expression (observed) of Beclin1

17) Confidence 0.05 Published 2009 Journal PLoS Pathogens Section Body Doc Link PMC2752191 Disease Relevance 0 Pain Relevance 0

(F) The superoxide generating agent menadione increases LC3-II and phosphorylation of JNK and Bcl-2, but has no effect on mTOR substrates or Beclin-1 expression.

Gene_expression (expression) of Beclin-1

18) Confidence 0.04 Published 2010 Journal Human Molecular Genetics Section Body Doc Link PMC2916709 Disease Relevance 0.06 Pain Relevance 0

Previous work has shown that oxidative stress in the form of hydrogen peroxide can increase the expression of Beclin-1 and thus induce autophagy (27).

Gene_expression (expression) of Beclin-1 associated with stress

19) Confidence 0.04 Published 2010 Journal Human Molecular Genetics Section Body Doc Link PMC2916709 Disease Relevance 0.17 Pain Relevance 0

We did not find that thiol antioxidants or vitamin E had any significant effect on levels of Beclin-1 (Fig. 6D and E).

Gene_expression (levels) of Beclin-1

20) Confidence 0.04 Published 2010 Journal Human Molecular Genetics Section Body Doc Link PMC2916709 Disease Relevance 0.16 Pain Relevance 0

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