INT238596

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Context Info
Confidence 0.44
First Reported 2008
Last Reported 2008
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 9
Disease Relevance 0.25
Pain Relevance 1.02

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleus (Sumo1) transcription factor binding (Sumo1) cytoplasm (Sumo1)
Anatomy Link Frequency
dendrites 1
spine 1
brain 1
Sumo1 (Rattus norvegicus)
Pain Link Frequency Relevance Heat
tetrodotoxin 90 90.80 High High
midbrain 18 88.84 High High
cerebral cortex 27 87.84 High High
Glutamate receptor 27 5.00 Very Low Very Low Very Low
Calcium channel 18 5.00 Very Low Very Low Very Low
Neurotransmitter 9 5.00 Very Low Very Low Very Low
GABAergic 9 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Adhesions 54 92.24 High High
Cytomegalovirus Infection 9 90.28 High High
Targeted Disruption 54 5.00 Very Low Very Low Very Low
Cleft Palate 18 5.00 Very Low Very Low Very Low
Myelodysplastic Syndromes 9 5.00 Very Low Very Low Very Low
Aids-related Complex 9 5.00 Very Low Very Low Very Low
Apoptosis 9 5.00 Very Low Very Low Very Low
Stroke 9 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To identify the SUMOylation site of CASK, a series of CASK domain deletion mutants (Fig. 4 A) were cotransfected with Myc-tagged SUMO1 into COS cells.
Gene_expression (cotransfected) of SUMO1
1) Confidence 0.44 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
Myc-SUMO1 expression plasmid was constructed by insertion of the SUMO1 fragment into a GW1 plasmid with the Myc cassette followed by RT-PCR amplification of SUMO1 cDNA from mouse brain RNA.
Gene_expression (expression) of SUMO1 in brain
2) Confidence 0.44 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
Under normal culture conditions, SUMO1 antibody did not give any obvious signal along the dendrites (Fig. 7 A, control panel).
Gene_expression (antibody) of SUMO1 in dendrites
3) Confidence 0.38 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0.21
Equal protein amounts of these fractions were then subjected to immunoprecipitation using CASK antibody followed by immunoblotting using SUMO1 and CASK antibodies sequentially.
Gene_expression (antibodies) of SUMO1
4) Confidence 0.38 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0.12
Among these synaptic SUMO1 puncta, ?
Gene_expression (puncta) of SUMO1
5) Confidence 0.38 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0.52
The pairs of primers used for construction of C- and CN-SUMO1-CASK were as follows: (1) for the fragment in which SUMO1 was deleted in the C-terminal GG region, 5?
Gene_expression (deleted) of SUMO1
6) Confidence 0.38 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0.09 Pain Relevance 0
Overexpression of a CASK–SUMO1 fusion construct, which mimicks CASK SUMOylation, impairs spine formation.
Gene_expression (Overexpression) of SUMO1 in spine
7) Confidence 0.38 Published 2008 Journal The Journal of Cell Biology Section Abstract Doc Link PMC2447900 Disease Relevance 0.16 Pain Relevance 0
In extracts that did not include N-ethylmaleimide to prevent deSUMOylation, the protein species at 140–150 kD could not be detected by either SUMO1 or CASK antibody (Fig. 6 A, right; ?
Gene_expression (antibody) of SUMO1
8) Confidence 0.38 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0.17
Although <10% of CASK protein was SUMOylated in COS cells (Figs. 4 B and 5 C, input lanes), SUMO1 overexpression resulted in a 60% reduction of the interaction between CASK and protein 4.1N (Fig. 5 C).
Gene_expression (overexpression) of SUMO1
9) Confidence 0.34 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0

General Comments

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