INT238608

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Context Info
Confidence 0.73
First Reported 2008
Last Reported 2008
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 41
Disease Relevance 2.74
Pain Relevance 1.90

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytosol (Cask) nucleolus (Cask) plasma membrane (Cask)
nucleus (Cask) intracellular (Cask) protein complex assembly (Cask)
Anatomy Link Frequency
spine 10
neurons 3
cerebral cortex 2
plasma 1
LS1 1
Cask (Rattus norvegicus)
Pain Link Frequency Relevance Heat
cerebral cortex 123 99.70 Very High Very High Very High
tetrodotoxin 410 89.52 High High
midbrain 82 88.48 High High
GABAergic 41 88.40 High High
Calcium channel 82 86.76 High High
Glutamate receptor 123 73.72 Quite High
Neurotransmitter 41 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Targeted Disruption 246 100.00 Very High Very High Very High
Apoptosis 41 95.16 Very High Very High Very High
Adhesions 246 92.24 High High
Cleft Palate 82 90.40 High High
Cytomegalovirus Infection 41 80.56 Quite High
Aids-related Complex 41 78.12 Quite High
Myelodysplastic Syndromes 41 5.00 Very Low Very Low Very Low
Stroke 41 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Similar to the results using CASK shRNA, overexpression of the CASK PDZ domain also resulted in fewer, shorter, and smaller spines compared with vector control (KS test; for density, D = 0.456 and P < 0.001; for length, D = 0.135 and P = 0.011; for width, D = 0.115 and P = 0.047; Fig. 3, A and B).
Gene_expression (overexpression) of CASK in spines
1) Confidence 0.73 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
However, CASK overexpression did not appear to further enlarge spine heads (Fig. 3, C and D).
Gene_expression (overexpression) of CASK in spine
2) Confidence 0.73 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
In the cerebral cortex, some neurons express higher levels of CASK, whereas other neurons express very low levels of CASK (Hsueh et al., 1998).
Gene_expression (express) of CASK in cerebral cortex associated with cerebral cortex
3) Confidence 0.73 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0.33 Pain Relevance 0.11
Overexpression of the CASK PDZ domain or CASK?
Gene_expression (Overexpression) of CASK
4) Confidence 0.73 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
Similar to the results in Fig. 3 (C and D), overexpression of CASK significantly increased the spine density compared with vector control (D = 0.334 and P < 0.001; Fig. 8).
Gene_expression (overexpression) of CASK in spine
5) Confidence 0.73 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
Our analysis of the time course of RNA interference and CASK overexpression experiments further suggests that CASK stabilizes or maintains spine morphology.
Gene_expression (overexpression) of CASK in spine
6) Confidence 0.73 Published 2008 Journal The Journal of Cell Biology Section Abstract Doc Link PMC2447900 Disease Relevance 0.16 Pain Relevance 0
In the cerebral cortex, some neurons express higher levels of CASK, whereas other neurons express very low levels of CASK (Hsueh et al., 1998).
Gene_expression (express) of CASK in cerebral cortex associated with cerebral cortex
7) Confidence 0.73 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0.32 Pain Relevance 0.09
Whether SUMO1, SUMO2, or SUMO3 are conjugated to CASK proteins was investigated by cotransfecting CASK and Myc-tagged SUMOs into COS cells followed by immunoprecipitation using CASK antibody.
Gene_expression (cotransfecting) of CASK
8) Confidence 0.73 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
Because CASK and protein 4.1 are widely expressed in different tissues, SUMOylation of CASK may also regulate the interaction between CASK and protein 4.1 in nonneuronal cells.
Gene_expression (expressed) of CASK
9) Confidence 0.73 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0.16 Pain Relevance 0
Overexpression of the CASK PDZ domain or CASK?
Gene_expression (Overexpression) of CASK
10) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
Compared with wild-type CASK or vector control, the expression of C-SUMO1-CASK significantly narrowed the width (GFP-actin + C-SUMO1-CASK vs.
Gene_expression (expression) of SUMO1-CASK
11) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0.19
At 16 DIV, CASK shRNA–expressing cells still had fewer spines along dendrites (KS test; D = 0.177 and P = 0.027; Fig. 2, B and C).
Gene_expression (expressing) of CASK shRNA in spines
12) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
Instead, a CASK mutant lacking the protein 4.1–binding motif was introduced into cultured hippocampal neurons (Fig. 3 A), and quantitative analyses showed that compared with GFP alone, overexpression of this CASK?
Gene_expression (overexpression) of CASK in neurons
13) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
When CASK was overexpressed, the spine density was increased (nonsilencing control + vector control vs. nonsilencing control + CASK; D = 0.406 and P < 0.001; Fig. 3, C and D).
Gene_expression (overexpressed) of CASK in spine
14) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
To identify the SUMOylation site of CASK, a series of CASK domain deletion mutants (Fig. 4 A) were cotransfected with Myc-tagged SUMO1 into COS cells.
Gene_expression (cotransfected) of CASK
15) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
At 15 DIV, although CASK protein levels had been knocked down by shRNA (Fig.
Gene_expression (knocked) of CASK
16) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
These results suggested that the PDZ domain and protein 4.1–binding sites of CASK have to be present in the same molecule to perform their functions in spine morphology.
Gene_expression (present) of CASK in spine
17) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
Consistent with the phenotype of mice carrying an insertional mutation in the CASK gene (Laverty and Wilson, 1998), deletion of the CASK gene results in lethality within 1 d after birth.
Gene_expression (deletion) of CASK
18) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0.16 Pain Relevance 0.12
In addition to 110-kD full-length CASK protein, an extra protein species at ?
Gene_expression (protein) of CASK
19) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0
In extracts that did not include N-ethylmaleimide to prevent deSUMOylation, the protein species at 140–150 kD could not be detected by either SUMO1 or CASK antibody (Fig. 6 A, right; ?
Gene_expression (antibody) of CASK
20) Confidence 0.64 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2447900 Disease Relevance 0 Pain Relevance 0.17

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