INT240173

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Context Info
Confidence 0.74
First Reported 2008
Last Reported 2008
Negated 2
Speculated 5
Reported most in Body
Documents 1
Total Number 72
Disease Relevance 42.06
Pain Relevance 0.42

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleus (Chd5) helicase activity (Chd5) molecular_function (Chd5)
cellular_component (Chd5) biological_process (Chd5)
Anatomy Link Frequency
SK-N-FI 5
SK-N-SH 4
brain 3
neurite 1
neural 1
Chd5 (Mus musculus)
Pain Link Frequency Relevance Heat
anesthesia 72 92.08 High High
ketamine 72 87.76 High High
Disease Link Frequency Relevance Heat
Cancer 4896 100.00 Very High Very High Very High
Neuroblastoma 4536 100.00 Very High Very High Very High
Necrosis 72 95.56 Very High Very High Very High
Death 144 94.88 High High
Apoptosis 288 94.20 High High
Body Weight 72 93.92 High High
Dislocations 72 91.20 High High
Recurrence 72 88.24 High High
Disease 288 84.56 Quite High
Solid Tumor 72 66.32 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Thus, CHD5 expression caused a statistically significant reduction in colony formation in the CHD5-expressing clones relative to the CHD5-AS clones, but only in cell lines with 1p deletion.
Gene_expression (expression) of CHD5
1) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.45 Pain Relevance 0
M), and assessed cell morphology (cell size, shape, and neurite outgrowth), cell number measured by MTT assay, and CHD5 expression by real-time reverse transcription–polymerase chain reaction (RT-PCR) after 0, 1, 4, and 7 days.


Gene_expression (expression) of CHD5 in neurite
2) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.30 Pain Relevance 0
A dose- and time-dependent increase in expression was observed in all lines that was most dramatic (one to two orders of magnitude) in the two cell lines with 1p deletion and no CHD5 expression, compared with only two- to fourfold in the cell lines with readily detectable CHD5 expression (data not shown).
Gene_expression (expression) of CHD5
3) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.13 Pain Relevance 0
Therefore, we investigated whether CHD5 expression was silenced by methylation in these lines.
Spec (whether) Gene_expression (expression) of CHD5
4) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.35 Pain Relevance 0
CHD5 expression was confirmed in the neuroblastoma xenografts by RT-PCR.
Gene_expression (expression) of CHD5 associated with neuroblastoma
5) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.69 Pain Relevance 0.11
The distal site showed much lower or no methylation in the two cell lines with low CHD5 expression (Figure 1, C and D) or in human fetal brain (data not shown).
Gene_expression (expression) of CHD5 in brain
6) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.13 Pain Relevance 0
We also examined CHD5 protein expression in the NLF and IMR5 lines.
Spec (examined) Gene_expression (expression) of CHD5
7) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 1.10 Pain Relevance 0
Mean values for CHD5 expression are presented with 95% confidence intervals.
Gene_expression (expression) of CHD5
8) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.68 Pain Relevance 0.14
A dose- and time-dependent increase in expression was observed in all lines that was most dramatic (one to two orders of magnitude) in the two cell lines with 1p deletion and no CHD5 expression, compared with only two- to fourfold in the cell lines with readily detectable CHD5 expression (data not shown).
Neg (no) Gene_expression (expression) of CHD5
9) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.16 Pain Relevance 0
CHD5 expression was strongly associated with increased event-free and overall survival of neuroblastoma patients.ImplicationsCHD5 may be a neuroblastoma tumor suppressor gene, and its expression may be inhibited by promoter methylation.LimitationsOther genes that are located in the region of the 1p deletions still need to be studied.
Gene_expression (expression) of CHD5 associated with neuroblastoma and cancer
10) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 1.28 Pain Relevance 0
These cell lines were also used to study the effect of exogenous CHD5 expression on in vitro growth, clonogenicity, and tumorigenicity.
Gene_expression (expression) of CHD5
11) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.30 Pain Relevance 0
Stable clones expressing CHD5 were selected and expanded and then seeded into T75 culture flasks and grown to 60%–70% confluency.
Gene_expression (expressing) of CHD5
12) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.17 Pain Relevance 0
We determined the methylation status of the CHD5 promoter in four cell lines—two with no CHD5expression (both with 1p deletion: NLF, IMR5) and two with low CHD5 expression (both without 1p deletion: SK-N-SH, S-KN-FI)—and in human fetal brain, which has high CHD5 expression.
Gene_expression (expression) of CHD5 in brain
13) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.21 Pain Relevance 0
Next, we injected neuroblastoma cells stably expressing CHD5 or CHD5-AS into the flanks of immunosuppressed nu/nu mice.
Gene_expression (expressing) of CHD5 associated with neuroblastoma
14) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.47 Pain Relevance 0
We analyzed CHD5 expression of these 24 lines by RT-PCR using CHD5 primers provided by the manufacturer (Hs00395930_m1) and the TaqMan Applied Biosystems 7900HT real-time RT-PCR system (Applied Biosystems Inc.
Spec (analyzed) Gene_expression (expression) of CHD5
15) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.29 Pain Relevance 0
We grew these lines in increasing concentrations of 5-aza-2-deoxycytidine for up to 7 days and measured normalized CHD5 expression using quantitative RT-PCR.
Gene_expression (expression) of CHD5
16) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.11 Pain Relevance 0
Thus, there was a strong association between methylation of the distal promoter site and CHD5 expression, although no well-established regulatory motifs were apparent.
Gene_expression (expression) of CHD5
17) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.20 Pain Relevance 0
Next, we injected neuroblastoma cells stably expressing CHD5 or CHD5-AS into the flanks of immunosuppressed nu/nu mice.
Gene_expression (expressing) of CHD5 associated with neuroblastoma
18) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.47 Pain Relevance 0
Expression of CHD5 was confirmed by RT-PCR analysis.
Gene_expression (Expression) of CHD5
19) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.18 Pain Relevance 0
CHD5 protein expression in NLF and IMR5 parental and transfected lines was detected by immunoblotting with rabbit polyclonal anti-CHD5 antiserum (1?
Gene_expression (expression) of CHD5
20) Confidence 0.74 Published 2008 Journal JNCI Journal of the National Cancer Institute Section Body Doc Link PMC2483574 Disease Relevance 0.13 Pain Relevance 0

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