INT244385

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Context Info
Confidence 0.77
First Reported 2008
Last Reported 2008
Negated 0
Speculated 0
Reported most in Body
Documents 12
Total Number 12
Disease Relevance 4.67
Pain Relevance 2.74

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

plasma membrane (Slc12a2)
Anatomy Link Frequency
neurons 3
spinal 1
neurite 1
Slc12a2 (Rattus norvegicus)
Pain Link Frequency Relevance Heat
Inflammatory mediators 506 99.76 Very High Very High Very High
Hyperalgesia 146 99.12 Very High Very High Very High
substance P 110 97.80 Very High Very High Very High
GABA receptor 2 96.64 Very High Very High Very High
imagery 55 93.68 High High
Dorsal horn 25 93.56 High High
Inflammation 199 91.36 High High
bradykinin 44 90.76 High High
Neuropathic pain 29 89.88 High High
Dorsal horn neuron 11 88.96 High High
Disease Link Frequency Relevance Heat
INFLAMMATION 727 99.56 Very High Very High Very High
Hyperalgesia 174 99.12 Very High Very High Very High
Spinal Cord Injury 72 93.24 High High
Nociception 139 92.48 High High
Neuropathic Pain 81 89.88 High High
Ganglion Cysts 22 75.00 Quite High
Nervous System Injury 15 57.68 Quite High
Pain 27 54.76 Quite High
Injury 12 44.00 Quite Low
Targeted Disruption 12 14.48 Low Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The early rise of intracellular Cl- levels coincides with phosphorylation of NKCC1
Phosphorylation (phosphorylation) of NKCC1
1) Confidence 0.77 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.18 Pain Relevance 0.11
The antisera directed against NKCC1 (C-14 antibody) and p-NKCC1 showed a virtually perfect match in the DRG sections (Fig. 4A) indicating that both non-phosphorylated and phosphorylated forms of NKCC1 are present in most neurons.
Phosphorylation (phosphorylated) of NKCC1 in neurons
2) Confidence 0.77 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.35 Pain Relevance 0.17
To test whether NKCC1 phosphorylation may contribute to the early rise of Cl- levels (< 3 hr), we stained 9 DRG sections from 3 animals with an antibody raised specifically against the phosphorylated form of NKCC1 (p-NKCC1).
Phosphorylation (phosphorylation) of NKCC1
3) Confidence 0.77 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.17 Pain Relevance 0.08
Changes of Cl- regulation that led to disinhibition of these spinal neurons and to referred mechanical hyperalgesia involved a rapid and transient increase of NKCC1 phosphorylation followed by increased recruitment of NKCC1 protein to the plasma membrane within 3 hours [33].
Phosphorylation (phosphorylation) of NKCC1 in spinal associated with hyperalgesia
4) Confidence 0.77 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.42 Pain Relevance 0.30
The antisera directed against NKCC1 (C-14 antibody) and p-NKCC1 showed a virtually perfect match in the DRG sections (Fig. 4A) indicating that both non-phosphorylated and phosphorylated forms of NKCC1 are present in most neurons.
Phosphorylation (phosphorylated) of NKCC1 in neurons
5) Confidence 0.77 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.35 Pain Relevance 0.17
To test whether NKCC1 phosphorylation may contribute to the early rise of Cl- levels (< 3 hr), we stained 9 DRG sections from 3 animals with an antibody raised specifically against the phosphorylated form of NKCC1 (p-NKCC1).
Phosphorylation (phosphorylated) of NKCC1
6) Confidence 0.77 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.22 Pain Relevance 0.11
To test whether NKCC1 phosphorylation may contribute to the early rise of Cl- levels (< 3 hr), we stained 9 DRG sections from 3 animals with an antibody raised specifically against the phosphorylated form of NKCC1 (p-NKCC1).
Phosphorylation (phosphorylated) of p-NKCC1
7) Confidence 0.67 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.22 Pain Relevance 0.11
These data demonstrate that the inflammatory mediators induce the phosphorylation of NKCC1 in DRG neurons and suggest that this phosphorylation causes the early (< 3 hr) phase of enhanced Cl- accumulation observed by 2P-FLIM in response to the stimulus.


Phosphorylation (phosphorylation) of NKCC1 in neurons associated with inflammatory mediators
8) Confidence 0.59 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.40 Pain Relevance 0.23
This effect coincided with enhanced phosphorylation of the Na+-K+-2Cl- cotransporter NKCC1, suggesting that an increased activity of that transporter caused the early rise of intracellular Cl- levels.
Phosphorylation (phosphorylation) of NKCC1
9) Confidence 0.59 Published 2008 Journal Mol Pain Section Abstract Doc Link PMC2526990 Disease Relevance 0.65 Pain Relevance 0.39
Immunohistochemical experiments revealed that phosphorylation of the Na+-K+-2Cl- cotransporter NKCC1 coincides with this increase of Cl- levels.
Phosphorylation (phosphorylation) of NKCC1
10) Confidence 0.59 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.77 Pain Relevance 0.59
NKCC1 phosphorylation stimulates neurite growth of injured adult sensory neurons [24].
Phosphorylation (phosphorylation) of NKCC1 in neurite
11) Confidence 0.53 Published 2008 Journal Mol Pain Section Body Doc Link PMC2561007 Disease Relevance 0.95 Pain Relevance 0.41
The following antibodies and dilutions were used: goat anti-NKCC1, 1:20 (C-14, Santa Cruz Biotechnology, Heidelberg, #sc-21547), goat anti-NKCC1, 1:20 (N-16; Santa Cruz, #sc-21545), goat anti-KCC2, 1:20 (R-14, Santa Cruz, #sc-19420), sheep anti-p-NKCC1, 1:20 (directed against the phosphorylated sites Thr203+Thr207+Thr212 of human NKCC1, The University of Dundee, Scotland, UK), rabbit anti-substance P, 1:50 (PC232L, Oncogene Research, San Diego), and rabbit anti-CGRP, 1:50 (PC205L, Oncogene Research).
Phosphorylation (phosphorylated) of NKCC1 associated with substance p
12) Confidence 0.51 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0 Pain Relevance 0.05

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