INT244393

Context Info Confidence 0.65 First Reported 2008 Last Reported 2008 Negated 0 Speculated 1 Reported most in Body Documents 1 Total Number 5 Disease Relevance 1.92 Pain Relevance 1.12

plasma membrane (Slc12a2)

Anatomy Link Frequency spinal 2 neurons 2 Slc12a2 (Rattus norvegicus)

Pain Link Frequency Relevance Heat Inflammatory mediators 230 99.98 Hyperalgesia 60 99.36 imagery 25 94.04 Dorsal horn 10 94.04 Inflammation 90 91.96 bradykinin 20 91.12 Dorsal horn neuron 5 88.96 Root ganglion neuron 5 47.84 Central nervous system 5 38.56 substance P 50 30.40

Disease Link Frequency Relevance Heat INFLAMMATION 330 99.78 Hyperalgesia 60 99.36 Nociception 60 92.96 Ganglion Cysts 10 75.00 Neuropathic Pain 25 25.00 Targeted Disruption 5 14.48 Neurological Disease 5 6.72 Peripheral Arterial Disease 20 5.00 Pain 10 5.00 Neurogenic Inflammation 10 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Changes of Cl- regulation that led to disinhibition of these spinal neurons and to referred mechanical hyperalgesia involved a rapid and transient increase of NKCC1 phosphorylation followed by increased recruitment of NKCC1 protein to the plasma membrane within 3 hours [33].

Positive_regulation (increase) of Phosphorylation (phosphorylation) of NKCC1 in spinal associated with hyperalgesia

1) Confidence 0.65 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.42 Pain Relevance 0.31

To test whether NKCC1 phosphorylation may contribute to the early rise of Cl- levels (< 3 hr), we stained 9 DRG sections from 3 animals with an antibody raised specifically against the phosphorylated form of NKCC1 (p-NKCC1).

Spec (may) Positive_regulation (contribute) of Phosphorylation (phosphorylation) of NKCC1

2) Confidence 0.47 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.17 Pain Relevance 0.08

The early rise of intracellular Cl- levels coincides with phosphorylation of NKCC1

Positive_regulation (coincides) of Phosphorylation (phosphorylation) of NKCC1

3) Confidence 0.47 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.23 Pain Relevance 0.12

This effect coincided with enhanced phosphorylation of the Na+-K+-2Cl- cotransporter NKCC1, suggesting that an increased activity of that transporter caused the early rise of intracellular Cl- levels.

Positive_regulation (enhanced) of Phosphorylation (phosphorylation) of NKCC1

4) Confidence 0.44 Published 2008 Journal Mol Pain Section Abstract Doc Link PMC2526990 Disease Relevance 0.70 Pain Relevance 0.39

These data demonstrate that the inflammatory mediators induce the phosphorylation of NKCC1 in DRG neurons and suggest that this phosphorylation causes the early (< 3 hr) phase of enhanced Cl- accumulation observed by 2P-FLIM in response to the stimulus.

Positive_regulation (induce) of Phosphorylation (phosphorylation) of NKCC1 in neurons associated with inflammatory mediators

5) Confidence 0.44 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.40 Pain Relevance 0.23

General Comments

This test has worked.