INT245173
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
However, the high expression levels of Midkine observed in the pedicle skin deserves some comment. | |||||||||||||||
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The only exception is Midkine, which was significantly overexpressed in the velvet respect to frontal skin samples, though not respect to pedicle skin. | |||||||||||||||
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According to present data, among the factors identified in the antler, only Midkine is significantly overexpressed in the antler velvet with respect to normal skin, suggesting a possible role promoting fast axonal growth. | |||||||||||||||
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Real time PCR data showed detectable gene expression (cycle threshold -Ct- below 34/35 cycles) for BDNF, BMP2, FGF2, GPI, L1CAM, Laminin B1 (LAMB1), Meteorin, Midkine, NR-CAM, and TGF? | |||||||||||||||
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Somehow, Midkine expression profile fits with what can be expected from a molecule involved in the process of rapid axonal growth in the antler. | |||||||||||||||
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Only Midkine expression appeared significantly upregulated in velvet compared to frontal samples, although it remained unchanged compared to pedicle. | |||||||||||||||
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We showed that most human pancreatic cancer cell lines (6 out of 7 lines tested) express midkine to some extent and that the level of midkine mRNA expression in pancreatic cancer tissues is significantly higher than in non-cancerous pancreatic tissues. | |||||||||||||||
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Sequencing confirmed the expression of 9 out of the 11 genes being analysed, namely BDNF, BMP2, FGF2, GPI, Midkine, Meteorin, NR-CAM and TGF? | |||||||||||||||
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These analyses yielded positive results for 8 of these genes, coding for BDNF, Midkine, basic Fibroblast Growth Factor (bFGF, also known as FGF2), BMP2 and TGF? | |||||||||||||||
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Midkine expression was increased in pancreatic cancer cell lines and pancreatic cancer tissues. | |||||||||||||||
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We showed that most human pancreatic cancer cell lines (6 out of 7 lines tested) express midkine to some extent and that the level of midkine mRNA expression in pancreatic cancer tissues is significantly higher than in non-cancerous pancreatic tissues. | |||||||||||||||
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Midkine expression in human pancreatic cancer | |||||||||||||||
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To assess midkine gene expression, we used quantitative real-time RT-PCR analysis based on the TaqMan fluorescence method, which employs a dual-labeled non-extendable oligonucleotide hydrolysis (TaqMan) probe in addition to the two amplification primers. | |||||||||||||||
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Adenoviral replication in AsPC-1 cells was lower than in any other midkine-positive cell line and no killing of AsPC-1 cells was observed at 10 MOI, although these cells showed high midkine expression and transcriptional activity. | |||||||||||||||
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CFPAC-1 cells had high midkine expression and moderate efficiency of adenoviral transduction. | |||||||||||||||
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Ad5MK showed a much stronger-killing effect against Suit-2 and PANC-1 cells that have moderate and weak midkine expression, respectively, compared with its effect on midkine-negative MIAPaCa-2 cells. | |||||||||||||||
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Expression of midkine mRNA was significantly stronger in pancreatic cancer than in non-cancerous tissue (p < 0.001).
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Therefore, to determine the specificity of Ad5MK replication, we used pancreatic cancer cell lines with different levels of midkine expression and then examined E1A expression by Western blotting (Figure 3A). | |||||||||||||||
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In contrast, Ad5MK had no effect on AsPC-1 cells as far as the designated conditions, although these cells showed strong midkine expression. | |||||||||||||||
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We assessed the expression of midkine mRNA in 22 pancreatic cancer samples and 18 adjacent non-cancerous pancreatic tissue samples by TaqMan PCR (Figure 1B). | |||||||||||||||
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