INT247722

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Context Info
Confidence 0.36
First Reported 2008
Last Reported 2008
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 20
Disease Relevance 1.34
Pain Relevance 0.54

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

extracellular region (Rarres2)
Anatomy Link Frequency
mast cells 3
macrophages 1
F4/80 1
Rarres2 (Mus musculus)
Pain Link Frequency Relevance Heat
chemokine 660 99.66 Very High Very High Very High
Potency 40 99.24 Very High Very High Very High
Bioavailability 20 92.24 High High
agonist 20 53.44 Quite High
Inflammation 160 42.72 Quite Low
cytokine 120 5.00 Very Low Very Low Very Low
Inflammatory response 60 5.00 Very Low Very Low Very Low
ketamine 20 5.00 Very Low Very Low Very Low
anesthesia 20 5.00 Very Low Very Low Very Low
Multiple sclerosis 20 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Anaphylaxis 580 97.08 Very High Very High Very High
Edema 380 93.92 High High
INFLAMMATION 220 42.72 Quite Low
Contact Dermatitis 140 41.00 Quite Low
Hypersensitivity 40 37.16 Quite Low
Disease 40 5.00 Very Low Very Low Very Low
Demyelinating Disease 20 5.00 Very Low Very Low Very Low
Overdose 20 5.00 Very Low Very Low Very Low
Lymphatic System Cancer 20 5.00 Very Low Very Low Very Low
Cancer 20 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Chemerin inhibits the binding of mAbs specific to amino-terminal epitopes of mCCRL2, implying that chemerin binds directly to the amino-terminal domain of the receptor.
chemerin Binding (binds) of
1) Confidence 0.36 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0.05
After chemerin binding, the cells were stained with directly conjugated F4/80 and c-kit mAbs and analyzed by flow cytometry.


chemerin Binding (binding) of in F4/80
2) Confidence 0.31 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0
For chemerin binding/anti-mCCRL2 mAbs displacement assays, total peritoneal exudate cells were incubated with various concentrations of chemerin or CCL2 for 5 min on ice in binding buffer, washed with PBS, and stained with primary antibodies (either anti-mCCRL BZ2E3 or IgE, + Fc block) for 45 min on ice.
chemerin Binding (binding) of
3) Confidence 0.28 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0
Freshly isolated mouse peritoneal mast cells also bound to chemerin (Fig. 6 E), and there was no obvious preference for binding of the proform versus the active serum form (this was also observed in radioligand binding studies using L1.2 transfectants; not depicted).
chemerin Binding (bound) of in mast cells
4) Confidence 0.27 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0.07
In radioligand binding studies, the His8 tag had little effect on the potency of chemerin binding to mCCRL2 (EC50 = 0.8 nM); however, His8-tagged chemerin bound with 10-fold less potency to mCMKLR1 (EC50 = 26.3 nM; Fig.
chemerin Binding (bound) of associated with potency
5) Confidence 0.27 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0.10
Despite high-affinity binding to mCCRL2, chemerin failed to trigger intracellular calcium mobilization in mCCRL2/L1.2 transfectants (Fig. 7 A).
chemerin Binding (binding) of
6) Confidence 0.27 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0.03
In CCRL2 binding, however, the bioactive chemerin peptide was an inefficient competitor (EC50 could not be determined, Fig.
chemerin Binding (binding) of
7) Confidence 0.27 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0.09
S7, available at http://www.jem.org/cgi/content/full/jem.20080300/DC1), and mCRTH2-HA/L1.2 transfectants did not bind to chemerin (Fig. 6 D), demonstrating specificity for chemerin–CCRL2 interactions.
chemerin-CCRL2 Binding (interactions) of
8) Confidence 0.27 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0.03
Mast cell–expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis

Mast cells contribute importantly to both protective and pathological IgE-dependent immune responses.

chemerin Binding (binds) of in Mast cell associated with anaphylaxis
9) Confidence 0.27 Published 2008 Journal The Journal of Experimental Medicine Section Title Doc Link PMC2556791 Disease Relevance 0.27 Pain Relevance 0.04
Moreover, mouse peritoneal mast cells bound both human and mouse chemerin (Fig. 6 E).
chemerin Binding (bound) of in mast cells
10) Confidence 0.27 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0.06
For direct chemerin binding immunofluorescence assays, mCCRL2-HA, huCCRL2-HA, mCMKLR1-HA, and mCRTH2-HA L1.2 transfectants were incubated for 30 min on ice with 10 nM His8-tagged serum form human chemerin and the indicated concentration of untagged chemerin in binding buffer (PBS with 0.5% BSA and 0.02% azide).
chemerin Binding (binding) of
11) Confidence 0.24 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0
CCRL2 binds chemerin
chemerin Binding (binds) of
12) Confidence 0.24 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0.32 Pain Relevance 0.04
For direct chemerin binding immunofluorescence assays, mCCRL2-HA, huCCRL2-HA, mCMKLR1-HA, and mCRTH2-HA L1.2 transfectants were incubated for 30 min on ice with 10 nM His8-tagged serum form human chemerin and the indicated concentration of untagged chemerin in binding buffer (PBS with 0.5% BSA and 0.02% azide).
chemerin Binding (binding) of
13) Confidence 0.24 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0
For direct chemerin binding immunofluorescence assays, mCCRL2-HA, huCCRL2-HA, mCMKLR1-HA, and mCRTH2-HA L1.2 transfectants were incubated for 30 min on ice with 10 nM His8-tagged serum form human chemerin and the indicated concentration of untagged chemerin in binding buffer (PBS with 0.5% BSA and 0.02% azide).
chemerin Binding (assays) of
14) Confidence 0.24 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0
In contrast to other “silent” or professional chemokine interreceptors, chemerin binding does not trigger ligand internalization.
chemerin Binding (binding) of associated with chemokine
15) Confidence 0.23 Published 2008 Journal The Journal of Experimental Medicine Section Abstract Doc Link PMC2556791 Disease Relevance 0.26 Pain Relevance 0.05
In saturation binding studies, chemerin bound to mCCRL2 at a single binding site with a calculated kD of 1.6 nM (Fig. 6 C).
chemerin Binding (bound) of
16) Confidence 0.23 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0
Thus, it appears that CCRL2 binds and indeed concentrates chemerin on the cell surface.
chemerin Binding (binds) of
17) Confidence 0.23 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0
We developed an immunofluorescence-based chemerin-binding assay to evaluate chemerin binding by flow cytometry.
chemerin Binding (binding) of
18) Confidence 0.23 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0
We developed an immunofluorescence-based chemerin-binding assay to evaluate chemerin binding by flow cytometry.
chemerin Binding (binding) of
19) Confidence 0.23 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0 Pain Relevance 0
Perhaps mast cell–bound and then activated chemerin interacts with CMKLR1+ macrophages or other mononuclear cells that might be present in normal skin, and such cells in turn have effects that can enhance local vascular permeability.
chemerin Binding (interacts) of in macrophages
20) Confidence 0.23 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556791 Disease Relevance 0.49 Pain Relevance 0

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