INT249861

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Context Info
Confidence 0.60
First Reported 2008
Last Reported 2008
Negated 3
Speculated 0
Reported most in Body
Documents 1
Total Number 38
Disease Relevance 5.64
Pain Relevance 2.07

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

signal transduction (Gpr17) plasma membrane (Gpr17) signal transducer activity (Gpr17)
Anatomy Link Frequency
neurons 4
oligodendrocyte 4
brain 3
microglia 2
parenchyma 2
Gpr17 (Mus musculus)
Pain Link Frequency Relevance Heat
ischemia 608 99.68 Very High Very High Very High
cerebral cortex 76 98.38 Very High Very High Very High
agonist 494 98.24 Very High Very High Very High
Hippocampus 114 97.76 Very High Very High Very High
antagonist 304 97.36 Very High Very High Very High
Potency 38 83.68 Quite High
Glutamate 76 67.32 Quite High
ketamine 38 50.40 Quite High
anesthesia 38 48.60 Quite Low
imagery 266 42.96 Quite Low
Disease Link Frequency Relevance Heat
Middle Cerebral Artery Infarction 1102 99.78 Very High Very High Very High
Cv Unclassified Under Development 418 99.68 Very High Very High Very High
Death 456 99.20 Very High Very High Very High
Brain Hemorrhage 304 97.88 Very High Very High Very High
Immunization 38 97.08 Very High Very High Very High
Anaplastic Astrocytoma 76 88.28 High High
Injury 266 88.24 High High
Brain Injury 342 85.12 High High
Contagious Ecthyma 38 64.96 Quite High
Cv General 4 Under Development 38 63.76 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Starting from 48 h and highly evident seventy-two h after MCAo, a marked expression of GPR17 was found at the boundaries of the lesioned area (Figure 5F), but this time immunoreactivity was associated to cells also expressing IB4, a marker of activated microglia/macrophages (Figure 5G).
Gene_expression (expression) of GPR17 in microglia associated with middle cerebral artery infarction
1) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0.41 Pain Relevance 0
The following primers were used to detect the expression of rat GPR17: Fw 5?
Gene_expression (expression) of GPR17
2) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0.03
Based on previous data demonstrating that GPR17 is one of the 3 key genes expressed in human adult NPCs [12], we also looked at the presence of GPR17+ cells in classical mouse brain neurogenic areas, i.e., the subventricular zone (SVZ) of lateral ventricles (LV) and the dentate gyrus (DG) of hippocampus.
Gene_expression (expressed) of GPR17 in lateral ventricles associated with hippocampus
3) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0.08 Pain Relevance 0.09
Forty-eight hours after MCAo, a dramatic decrease of both the number of neuronal cells and of GPR17 immunoreactivity was found inside the lesion, suggesting that neurons over-expressing GPR17 at 24 h had undergone cell death (Figure 5D).
Gene_expression (expressing) of GPR17 in neurons associated with middle cerebral artery infarction and death
4) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0.43 Pain Relevance 0.07
We finally looked at the expression pattern of GPR17 in mice after induction of MCAo.
Gene_expression (expression) of GPR17 associated with middle cerebral artery infarction
5) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0.44 Pain Relevance 0.22
At this stage of damage development, GFAP+ activated astrocytes indicating astrogliosis were also visible at the borders of the injured area, but none of these cells expressed GPR17 (data not shown).
Neg (none) Gene_expression (expressed) of GPR17 in astrocytes
6) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0.41 Pain Relevance 0
In vivo, GPR17 is also present in both cells of the ependymal layer and in a subset of subependymal precursor cells of the lateral ventricles, one of the classical neurogenic areas that are still active in the adult brain.
Gene_expression (present) of GPR17 in adult brain
7) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0
Expression of GPR17 in oligodendrocyte precursors was also confirmed by single-cell PCR.
Gene_expression (Expression) of GPR17 in oligodendrocyte
8) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0
These cells never co-stained with either the stem cell marker nestin (not shown), GFAP (Figure 3B) or the neuronal precursor marker double-cortin (DCX) (Figure 3D), suggesting that GPR17 is not expressed by GFAP+ multipotent stem cells or by DCX+ neuronal precursors [19], [20], [21].
Neg (not) Gene_expression (expressed) of GPR17 in stem cell
9) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0.08
The following primers were used to detect the expression of mouse GPR17: Fw 5?
Gene_expression (expression) of GPR17
10) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0.10
In particular, 3 distinct phenotypes of GPR17+ ramified cells were found: a first cell type was characterized by exclusive expression of NG2 (arrowheads in Figure 2G), a second by exclusive expression of GPR17 (white arrows in Figure 2G) while a third cell type co-expressed both markers (blue arrows in Figure 2G and inset).
Gene_expression (expression) of GPR17
11) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0
To investigate in detail whether GPR17 is specifically expressed by pre-oligodendrocytes, we performed double staining experiments with a set of markers identifying different stages of differentiation, i.e., NG2 (oligodendrocyte precursor), O4 and CNPase (pre-oligodendrocytes, immature/mature cells), and MBP (mature oligodendrocytes) [22], [23].
Gene_expression (expressed) of GPR17 in oligodendrocytes
12) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0.04
Expression pattern of GPR17 during ischemia evolution
Gene_expression (Expression) of GPR17 associated with ischemia
13) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0.32 Pain Relevance 0.13
Finally, no GPR17 co-expression was found in resting microglia detected by anti-Iba1 staining in the intact brain (Figure 2K) (however, see: “Expression pattern of GPR17 during ischemia evolution”).
Gene_expression (co-expression) of GPR17 in microglia associated with ischemia
14) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0.09 Pain Relevance 0.05
On the other hand, in a recent transcriptome study comparing the gene expression pattern of human fetal and adult neuroprogenitor cells (NPCs), GPR17 was identified as one of the three genes that were exclusively expressed in adult hippocampal NPCs [12], thus highlighting a potential role in brain repair.
Gene_expression (expressed) of GPR17 in brain
15) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0.33 Pain Relevance 0.08
Based on the demonstration of GPR17 on NPCs [12] (Figure 3) and on the finding that ischemic damage induces proliferation of adult NPCs in brain's neurogenic areas and parenchyma [25], we investigated whether cells expressing GPR17 also underwent proliferation after MCAo, as assessed in vivo by BrdU administration.
Gene_expression (expressing) of GPR17 in parenchyma associated with middle cerebral artery infarction
16) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0.32 Pain Relevance 0
Several shrunk cells overexpressing GPR17 and resembling damaged neurons were also found at the borders of the ischemic infarct (Figure 5D).
Gene_expression (overexpressing) of GPR17 in neurons
17) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0.47 Pain Relevance 0.06
This suggests that GPR17 expression is specifically segregated to the early stages of the oligodendrocyte differentiating pathway, and that the receptor is turned down when these cells reach functional maturation (in this respect, see also below).
Gene_expression (expression) of GPR17 in oligodendrocyte
18) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0
The other cell type expressing GPR17 displayed small cell bodies with fine radiating processes (see arrows in Figure 2A, 2A?
Gene_expression (expressing) of GPR17
19) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0.04
GPR17 expression pattern was evaluated and confirmed on paraffin-embedded, frozen and floating brain sections.
Gene_expression (expression) of GPR17 in brain
20) Confidence 0.52 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0

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