INT250576

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Context Info
Confidence 0.77
First Reported 2008
Last Reported 2009
Negated 0
Speculated 1
Reported most in Body
Documents 3
Total Number 28
Disease Relevance 18.99
Pain Relevance 0.13

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleus (USF1) enzyme binding (USF1)
Anatomy Link Frequency
fat 2
plasma 1
USF1 (Homo sapiens)
Pain Link Frequency Relevance Heat
anesthesia 26 79.28 Quite High
Inflammation 28 24.12 Low Low
Angina 4 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Familial Combined Hyperlipidemia 3304 100.00 Very High Very High Very High
Disease 338 100.00 Very High Very High Very High
Disorder Of Lipid Metabolism 422 98.88 Very High Very High Very High
Coronary Artery Disease 148 98.40 Very High Very High Very High
Obesity 224 94.08 High High
Hyperlipidemia 130 86.40 High High
Myocardial Infarction 30 74.56 Quite High
Stroke 66 73.60 Quite High
Dyslipidemia /

Combined Dyslipidemia

130 69.76 Quite High
Cardiovascular Disease 140 68.96 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Transient transfection and over-expression of USF1 were conducted in HEK293T cells, which express the USF1 protein (Figure S1).
Gene_expression (expression) of USF1
1) Confidence 0.77 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.64 Pain Relevance 0
To test this hypothesis we first identified the direct and indirect targets of USF1 by transiently over-expressing USF1 in vitro and assaying for differential expression using gene expression microarrays.
Gene_expression (expressing) of USF1
2) Confidence 0.77 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.53 Pain Relevance 0
Previous studies involving direct sequencing, extensive genotyping and gene expression analyses of the USF1 region have not identified any SNPs in the rs3737787 LD bin altering the coding sequence or the expression of USF1 itself in fat [10] or lymphoblasts [11].
Gene_expression (expression) of USF1 in fat
3) Confidence 0.77 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.74 Pain Relevance 0
The transient transfection and over-expression of USF1 microarray data can be accessed in MIAME compliant format from NCBI GEO database (GSE17300).


Gene_expression (expression) of USF1
4) Confidence 0.77 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.26 Pain Relevance 0.08
Transient transfection and over-expression of USF1 were conducted in HEK293T cells, which express the USF1 protein (Figure S1).
Gene_expression (express) of USF1
5) Confidence 0.68 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.62 Pain Relevance 0
10) between the FCHL differentially expressed genes and the tan and midnightblue module genes (tan module overlap p-value?
Gene_expression (expressed) of FCHL associated with familial combined hyperlipidemia
6) Confidence 0.68 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 1.06 Pain Relevance 0
We then tested whether the two SNPs rs174547 and rs102275 predicted the expression of FADS1 and/or FADS3 in the Mexican FCHL case/control fat biopsies.
Gene_expression (expression) of FCHL in fat associated with familial combined hyperlipidemia
7) Confidence 0.68 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.73 Pain Relevance 0
Although our current study does not allow us to pinpoint which of these three USF1 SNPs in LD is the actual causative SNP, we were able to demonstrate that an allele-dependent effect of the rs3737787 tagged LD bin on downstream genes is mediated by USF1 and not the adjacent gene F11R.
Gene_expression (three) of USF1
8) Confidence 0.67 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.63 Pain Relevance 0
Differential expression between USF1 and empty vector control transfected cells was analyzed using a Student's T-test (Table S1).
Spec (analyzed) Gene_expression (expression) of USF1
9) Confidence 0.67 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.29 Pain Relevance 0.05
Although our current study does not allow us to pinpoint which of these three USF1 SNPs in LD is the actual causative SNP, we were able to demonstrate that an allele-dependent effect of the rs3737787 tagged LD bin on downstream genes is mediated by USF1 and not the adjacent gene F11R.
Gene_expression (which) of USF1
10) Confidence 0.67 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.69 Pain Relevance 0
The rs3737787 correlated genes had significant overlap both with i) the set of USF1 regulated genes identified in our USF1 over-expression experiment (n?
Gene_expression (expression) of USF1
11) Confidence 0.67 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.22 Pain Relevance 0
Although our current study does not allow us to pinpoint which of these three USF1 SNPs in LD is the actual causative SNP, we were able to demonstrate that an allele-dependent effect of the rs3737787 tagged LD bin on downstream genes is mediated by USF1 and not the adjacent gene F11R.
Gene_expression (these) of USF1
12) Confidence 0.67 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.68 Pain Relevance 0
In addition to USF1 variant rs2774279, also the T allele of variant rs2073658 of USF1 conferred risk in both incident and prevalent female cases (HR?
Gene_expression (rs2774279) of USF1
13) Confidence 0.62 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2574036 Disease Relevance 0.66 Pain Relevance 0
In addition to USF1 variant rs2774279, also the T allele of variant rs2073658 of USF1 conferred risk in both incident and prevalent female cases (HR?
Gene_expression (rs2774279) of USF1
14) Confidence 0.62 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2574036 Disease Relevance 0.71 Pain Relevance 0
Over-expression of USF1 was validated by western blot using Anti-HisG-HRP (Invitrogen, Cat No.
Gene_expression (expression) of USF1
15) Confidence 0.60 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.27 Pain Relevance 0
We transiently over-expressed USF1 in the HEK293T cell line and assayed for differential expression using gene expression microarrays to detect both direct and indirect targets of USF1.
Gene_expression (expressed) of USF1
16) Confidence 0.60 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.53 Pain Relevance 0
We hypothesized that if the SNP rs3737787 tagged LD bin alters the function of the transcription factor USF1, the effect could be assayed on the downstream expression of USF1 target genes, or the subsequent targets of those genes.
Gene_expression (expression) of USF1
17) Confidence 0.60 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.51 Pain Relevance 0
Over-expression of USF1 was validated by western blot (Figure S1).
Gene_expression (expression) of USF1
18) Confidence 0.60 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 0.40 Pain Relevance 0
Based on a USF1-regulated FCHL-associated co-expression module and SNP rs3737787, we identify a set of causal candidate genes for FCHL-related traits.
Gene_expression (expression) of FCHL associated with familial combined hyperlipidemia
19) Confidence 0.59 Published 2009 Journal PLoS Genetics Section Abstract Doc Link PMC2730565 Disease Relevance 1.09 Pain Relevance 0
Given that FCHL is known to be a complex disease, it is not surprising that FCHL status is associated with multiple modules.
Gene_expression (disease) of FCHL associated with familial combined hyperlipidemia and disease
20) Confidence 0.59 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2730565 Disease Relevance 1.06 Pain Relevance 0

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