INT251426

Context Info Confidence 0.22 First Reported 2008 Last Reported 2008 Negated 0 Speculated 1 Reported most in Body Documents 1 Total Number 6 Disease Relevance 0 Pain Relevance 0.35

mitochondrion (Aldh2) signal transduction (Gnat3) oxidoreductase activity (Aldh2) GTPase activity (Gnat3) cytoplasm (Gnat3) signal transducer activity (Gnat3)

Gnat3 (Mus musculus) Aldh2 (Rattus norvegicus)

Pain Link Frequency Relevance Heat tolerance 84 99.28 addiction 30 79.60 Angina 6 5.00

Disease Link Frequency Relevance Heat Increased Venous Pressure Under Development 12 48.76 Stress 6 5.00 Cv General 3 Under Development 6 5.00

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Inhibition of Dehydrogenase Activity by GTN—To study the inactivation of ALDH2 by GTN in real time we monitored the formation of NADH from NAD+ spectrophotometrically at 340 nm and determined how the activity of ALDH2 was affected by the addition of GTN.

GTN Negative_regulation (inactivation) of ALDH2

1) Confidence 0.22 Published 2008 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2576553 Disease Relevance 0 Pain Relevance 0

Determination of the Inactivation Kinetics—To determine the rate of inactivation of ALDH2 by GTN, the optical absorption of a 1-cm quartz cuvette (type 105.200-QS, Hellma, Müllheim, Germany) containing substrate (acetaldehyde or p-NPA) and additional compounds like NAD+ or DTT as indicated in 50 mm potassium Pi (pH 7.4), was monitored at 340 (dehydrogenase) or 400 nm (esterase) in a Hewlett-Packard 8452A Diode Array spectrophotometer.

GTN Negative_regulation (inactivation) of ALDH2

2) Confidence 0.22 Published 2008 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2576553 Disease Relevance 0 Pain Relevance 0.03

In the present paper we investigated the inactivation of ALDH2 by GTN in the presence and absence of thiols to elucidate this issue.

GTN Spec (investigated) Negative_regulation (inactivation) of ALDH2

3) Confidence 0.22 Published 2008 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2576553 Disease Relevance 0 Pain Relevance 0.23

Mitochondrial aldehyde dehydrogenase (ALDH2) may be involved in the biotransformation of glyceryl trinitrate (GTN), and the inactivation of ALDH2 by GTN may contribute to the phenomenon of nitrate tolerance.

GTN Negative_regulation (inactivation) of ALDH2 associated with tolerance

4) Confidence 0.22 Published 2008 Journal The Journal of Biological Chemistry Section Abstract Doc Link PMC2576553 Disease Relevance 0 Pain Relevance 0.05

We studied the GTN-induced inactivation of ALDH2 by UV/visible absorption spectroscopy.

GTN Negative_regulation (inactivation) of ALDH2

5) Confidence 0.19 Published 2008 Journal The Journal of Biological Chemistry Section Abstract Doc Link PMC2576553 Disease Relevance 0 Pain Relevance 0.05

Inactivation of ALDH2 by Preincubation with GTN (“GTN Preconditioning”)—Purified ALDH2 (0.27 mg/ml) was preincubated with 1 ?

GTN Negative_regulation (Inactivation) of ALDH2

6) Confidence 0.19 Published 2008 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2576553 Disease Relevance 0 Pain Relevance 0

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