INT252124

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Context Info
Confidence 0.72
First Reported 2008
Last Reported 2011
Negated 0
Speculated 0
Reported most in Body
Documents 21
Total Number 21
Disease Relevance 1.56
Pain Relevance 2.71

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

endosome (Snap25) plasma membrane (Snap25) cytoplasm (Snap25)
Anatomy Link Frequency
Purkinje cells 4
brain 4
adult brain 2
neuronal 1
vesicles 1
Snap25 (Mus musculus)
Pain Link Frequency Relevance Heat
Hippocampus 76 99.90 Very High Very High Very High
GABAergic 540 98.68 Very High Very High Very High
Pyramidal cell 112 96.28 Very High Very High Very High
Thalamus 49 93.96 High High
Neurotransmitter 148 75.52 Quite High
anesthesia 10 67.64 Quite High
Catecholamine 28 59.76 Quite High
Action potential 132 50.00 Quite Low
Neuropeptide 68 49.44 Quite Low
Calcium channel 96 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Adhesions 1 98.92 Very High Very High Very High
Targeted Disruption 105 95.68 Very High Very High Very High
Embryonic Lethality 8 83.68 Quite High
Convulsion 104 81.12 Quite High
Body Weight 33 73.68 Quite High
Sprains And Strains 28 52.48 Quite High
Attention Deficit Hyperactivity Disorder 40 44.32 Quite Low
Intellectual Impairment 8 33.28 Quite Low
Cognitive Disorder 56 5.00 Very Low Very Low Very Low
Anxiety Disorder 32 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
In order to determine the relative ratio of SNAP-25a and SNAP-25b mRNA expression in brain at PN14, isolated RNA from homozygous and heterozygous SNAP-25b deficient mutants and WT littermates was subjected to an RT-PCR assay based on the presence of exclusive restriction enzyme sites in exons 5a and 5b.
Transcription (expression) of SNAP-25a in brain
1) Confidence 0.72 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.05 Pain Relevance 0
In order to determine the relative ratio of SNAP-25a and SNAP-25b mRNA expression in brain at PN14, isolated RNA from homozygous and heterozygous SNAP-25b deficient mutants and WT littermates was subjected to an RT-PCR assay based on the presence of exclusive restriction enzyme sites in exons 5a and 5b.
Transcription (expression) of SNAP-25b in brain
2) Confidence 0.72 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.05 Pain Relevance 0
SNAP-25b mRNA in WT mice was, as expected, five times higher than SNAP-25a (Figure 2C).
Transcription (higher) of SNAP-25a
3) Confidence 0.69 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.28 Pain Relevance 0
In fact, in adult mouse brain the SNAP-25b transcript represents more than 90% of total SNAP-25 mRNA [26].
Transcription (transcript) of SNAP-25b in adult mouse
4) Confidence 0.69 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.25 Pain Relevance 0.03
In heterozygous neo-excised mutants the relative SNAP-25a/SNAP-25b mRNA ratio was 2?
Transcription (ratio) of SNAP-25b
5) Confidence 0.69 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.27 Pain Relevance 0
In heterozygous neo-excised mutants the relative SNAP-25a/SNAP-25b mRNA ratio was 2?
Transcription (ratio) of SNAP-25a
6) Confidence 0.69 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.27 Pain Relevance 0
SNAP-25b mRNA in WT mice was, as expected, five times higher than SNAP-25a (Figure 2C).
Transcription (higher) of SNAP-25b
7) Confidence 0.69 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.29 Pain Relevance 0
Determination of SNAP-25a/b mRNA ratio expression was essentially performed as described [26] (and Text S1).
Transcription (expression) of SNAP-25a
8) Confidence 0.69 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0 Pain Relevance 0.07
Comparing the two major neuronal populations of the cerebellum, the abundance of SNAP-25b transcripts in Purkinje cells was more than an 8.5-fold lower, relative to ?
Transcription (transcripts) of SNAP-25b in Purkinje cells
9) Confidence 0.69 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.29
Overall, the predominance of SNAP-25b transcripts amongst these adult brain regions selected by LCM (88–93% of the total) was consistent with the isoform transcript levels obtained for adult cortical cells isolated by FACS (Fig. 4C), extending these findings to neuronal populations in the hippocampus, cerebellum and the subcortical regions of the thalamus and basal ganglia.
Transcription (transcripts) of SNAP-25b in adult brain associated with thalamus and hippocampus
10) Confidence 0.69 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.35
However, as shown in Fig. 5, panels C and D, the expression level of both SNAP-25a and SNAP-25b transcripts did vary significantly between various brain regions (SNAP-25a, p < 0.001; SNAP-25b, p = 0.0002, one-way ANOVA).
Transcription (transcripts) of SNAP-25a in brain
11) Confidence 0.69 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.23
Moreover, SNAP-25a transcripts in Purkinje cells were also greatly decreased relative to GABAergic neurons of the reticular nucleus and the caudate, as well as hippocampal interneurons and pyramidal neurons (Fig. 5C).
Transcription (transcripts) of SNAP-25a in Purkinje cells associated with pyramidal cell and gabaergic
12) Confidence 0.69 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.23
However, as shown in Fig. 5, panels C and D, the expression level of both SNAP-25a and SNAP-25b transcripts did vary significantly between various brain regions (SNAP-25a, p < 0.001; SNAP-25b, p = 0.0002, one-way ANOVA).
Transcription (transcripts) of SNAP-25b in brain
13) Confidence 0.69 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.23
For example, in the hippocampus the abundance of SNAP-25 protein in presynaptic mossy fiber terminals of dentate gyrus granule neurons, which are highly active and contain a disproportionately large pool of releasable vesicles, appears much higher than in the terminal fields of neighboring pyramidal neurons [16,36], despite apparent lower levels of mRNA transcripts compared to CA3 pyramidal neurons.
Transcription (abundance) of SNAP-25 protein in vesicles associated with pyramidal cell and hippocampus
14) Confidence 0.69 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.20
In particular, although the relative levels of the isoform transcripts were similar, the major SNAP-25b RNA transcript, accounting for ~90% of the total SNAP-25 RNA, was significantly lower in GABAergic cerebellar Purkinje cells than other brain regions (Fig. 5D).
Transcription (transcript) of SNAP-25b in Purkinje cells associated with gabaergic
15) Confidence 0.69 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.20
In fact, cerebellar granule cells showed the most difference between these neuronal populations with SNAP-25a transcripts accumulating to only 7.0% of the total SNAP-25 RNA.
Transcription (transcripts) of SNAP-25a in neuronal
16) Confidence 0.69 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.18
As shown in Fig. 4D, in contrast to the greater abundance of SNAP-25b transcripts in adult brain, the level of the transcripts encoding the two isoforms was equivalent in both GABAergic and non-GABAergic populations of neonatal cortical cells.
Transcription (transcripts) of SNAP-25b in adult brain associated with gabaergic
17) Confidence 0.69 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.17
Using a more sensitive, quantitative qRT-PCR assay, we show here that the expression of SNAP-25a and 25b RNA transcripts compared to ?
Transcription (transcripts) of SNAP-25a
18) Confidence 0.69 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.11
To examine the level of SNAP-25 mRNA expression in the selected cell populations, we further compared the qRT-PCR amplification of SNAP-25 isoform transcripts to ?
Transcription (transcripts) of SNAP-25 isoform
19) Confidence 0.60 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.22
In particular, although the relative levels of the isoform transcripts were similar, the major SNAP-25b RNA transcript, accounting for ~90% of the total SNAP-25 RNA, was significantly lower in GABAergic cerebellar Purkinje cells than other brain regions (Fig. 5D).
Transcription (transcript) of SNAP-25 in Purkinje cells associated with gabaergic
20) Confidence 0.60 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.20

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