INT252133

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Context Info
Confidence 0.70
First Reported 2008
Last Reported 2008
Negated 1
Speculated 0
Reported most in Body
Documents 19
Total Number 19
Disease Relevance 2.36
Pain Relevance 1.35

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

endosome (Snap25) plasma membrane (Snap25) cytoplasm (Snap25)
Anatomy Link Frequency
brain 8
vesicles 6
granule cells 4
cleavage 2
neuroendocrine cells 2
Snap25 (Mus musculus)
Pain Link Frequency Relevance Heat
Catecholamine 26 98.76 Very High Very High Very High
GABAergic 450 98.20 Very High Very High Very High
Neuropeptide 66 93.88 High High
Neurotransmitter 126 87.60 High High
gABA 10 86.48 High High
Glutamate 22 85.76 High High
Hippocampus 70 74.40 Quite High
Action potential 110 50.00 Quite Low
Pyramidal cell 96 29.40 Quite Low
Calcium channel 80 26.08 Quite Low
Disease Link Frequency Relevance Heat
Targeted Disruption 97 99.96 Very High Very High Very High
Embryonic Lethality 8 99.36 Very High Very High Very High
Diabetes Mellitus 154 84.44 Quite High
Attention Deficit Hyperactivity Disorder 40 71.36 Quite High
Convulsion 104 70.76 Quite High
Tremor 16 68.44 Quite High
Body Weight 32 63.32 Quite High
Intellectual Impairment 8 60.32 Quite High
Hyperglycemia 5 56.40 Quite High
Leukemia 11 45.32 Quite Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
In mouse brain, SNAP-25a precedes SNAP-25b expression during development, but by the second postnatal (PN) week SNAP-25b becomes the major splice variant, concomitantly with a dramatic increase in SNAP-25 expression [26].
Positive_regulation (increase) of Gene_expression (expression) of SNAP-25 in brain
1) Confidence 0.70 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.24 Pain Relevance 0.03
Separate overexpression of the two SNAP-25 isoforms in embryonic adrenal chromaffin cells from these SNAP-25 knock-out (KO) mice showed that the SNAP-25b isoform had a higher capability to stabilize the pool of primed vesicles than SNAP-25a, since the burst of Ca2+-evoked catecholamine release differed [28].
Positive_regulation (overexpression) of Gene_expression (overexpression) of SNAP-25 in vesicles associated with targeted disruption and catecholamine
2) Confidence 0.70 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.47 Pain Relevance 0.05
As shown in Fig. 6C, qRT-RCR was readily able to distinguish a 7.5-fold and 5-fold greater expression of SNAP-25a transcripts in young P24 (post-weaning) and adult (P124) knockin mice, respectively, relative to wild type, which is comparable to level of overexpression of SNAP-25a transcripts previously determined for these mice based on isoform-specific cleavage by restriction endonucleases of total SNAP-25 PCR amplified cDNA [see ref. [22]].
Positive_regulation (overexpression) of Gene_expression (overexpression) of SNAP-25a in cleavage
3) Confidence 0.70 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0
Depolarization of dentate gyrus granule cells has been shown to induce expression of SNAP-25b rather than SNAP-25a [48], whereas activation of neurosecretory magnocellular hypothalamic neurons has been reported to increase SNAP-25a expression exclusively [20].
Positive_regulation (increase) of Gene_expression (expression) of SNAP-25a in granule cells
4) Confidence 0.70 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.11
The reduced SNAP-25 expression in neo-containing SNAP-25b deficient mutants could be due to the presence of the Tkneo selection gene.
Positive_regulation (due) of Gene_expression (expression) of SNAP-25b
5) Confidence 0.50 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.14 Pain Relevance 0
The reduced SNAP-25 expression in neo-containing SNAP-25b deficient mutants could be due to the presence of the Tkneo selection gene.
Positive_regulation (due) of Gene_expression (expression) of SNAP-25
6) Confidence 0.50 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.14 Pain Relevance 0
Separate overexpression of the two SNAP-25 isoforms in embryonic adrenal chromaffin cells from these SNAP-25 knock-out (KO) mice showed that the SNAP-25b isoform had a higher capability to stabilize the pool of primed vesicles than SNAP-25a, since the burst of Ca2+-evoked catecholamine release differed [28].
Positive_regulation (overexpression) of Gene_expression (overexpression) of SNAP-25 in vesicles associated with targeted disruption and catecholamine
7) Confidence 0.50 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.49 Pain Relevance 0.05
In neo-excised mutants SNAP-25 protein expression was moderately but significantly increased to 111% compared to WT (Figure 2D).
Positive_regulation (increased) of Gene_expression (expression) of SNAP-25 protein
8) Confidence 0.50 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.25 Pain Relevance 0
Immunohistochemical analysis of SNAP-25 showed a similar appearance in homozygous neo-containing SNAP-25b deficient mutant and WT mouse brain at PN14, and no obvious pathological changes were observed (Figure 1E).
Positive_regulation (appearance) of Gene_expression (deficient) of SNAP-25b in brain
9) Confidence 0.50 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.05 Pain Relevance 0
Immunohistochemical analysis of SNAP-25 showed a similar appearance in homozygous neo-containing SNAP-25b deficient mutant and WT mouse brain at PN14, and no obvious pathological changes were observed (Figure 1E).
Positive_regulation (appearance) of Gene_expression (homozygous) of SNAP-25b in brain
10) Confidence 0.50 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0 Pain Relevance 0
To assess the ability to quantify SNAP-25 isoforms from brain tissue, we also compared amplification from cDNAs prepared from cortex of Snap25+/+ and a Snap25 knockin mutant (Snap25tkneo/tkneo) that overexpresses SNAP-25a transcripts [22].
Positive_regulation (overexpresses) of Gene_expression (overexpresses) of SNAP-25a in cortex
11) Confidence 0.50 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0
Consistent with this idea, expression of SNAP-25b leads to the greater recruitment of vesicles to the readily releasable pool in hippocampal neurons compared to SNAP-25a [11], and similarly stabilizes a larger pool of vesicles for catecholamine secretion in adrenal chromaffin cells [4].
Positive_regulation (stabilizes) of Gene_expression (expression) of SNAP-25b in vesicles associated with catecholamine
12) Confidence 0.50 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.15
In the series of experiments evaluating SNAP-25 isoform expression in cells harvested from different brain regions by LCM (Fig. 5), the Ct values for ?
Positive_regulation (evaluating) of Gene_expression (expression) of SNAP-25 isoform in brain
13) Confidence 0.50 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0
Relative expression of SNAP-25 isoforms does not vary in different anatomical regions
Neg (not) Positive_regulation (vary) of Gene_expression (expression) of SNAP-25 in anatomical regions
14) Confidence 0.50 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.30
Consistent with previous studies [15,16], we find a similar developmental profile of SNAP-25 isoforms in GABAergic (GFPpos) and nonGABAergic (GFPneg, glutamatergic) neurons in the cortex with equal representation of SNAP-25a and -25b transcripts in neonates leading to the predominant expression of SNAP-25b in the adult.
Positive_regulation (leading) of Gene_expression (expression) of SNAP-25b in neurons associated with gabaergic
15) Confidence 0.47 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.27
Depolarization of dentate gyrus granule cells has been shown to induce expression of SNAP-25b rather than SNAP-25a [48], whereas activation of neurosecretory magnocellular hypothalamic neurons has been reported to increase SNAP-25a expression exclusively [20].
Positive_regulation (induce) of Gene_expression (expression) of SNAP-25a in granule cells
16) Confidence 0.47 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.14
Neurons in these areas maybe more comparable to other neuroendocrine cells, such as adrenal gland and pancreatic beta cells, that persistently express high levels of SNAP-25a in the adult [17,18,47], suggesting that the preference for one isoform may reflect a mechanism that tailors exocytotic machinery to secretory properties.
Positive_regulation (levels) of Gene_expression (express) of SNAP-25a in neuroendocrine cells
17) Confidence 0.47 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.15
While it has been demonstrated that SNAP-25 is expressed and required for stimulus-driven synaptic transmission by both glutamatergic and GABAergic neurons in fetal mouse brain and in culture [3,5,10,11], it has been also proposed that differential expression of this t-SNARE may lead to differences in calcium dynamics between inhibitory and excitatory synapses [8] and thereby possibly contribute to the physiological diversity observed between these neurons [for review, see [29]].
Positive_regulation (required) of Gene_expression (expressed) of SNAP-25 in GABAergic neurons associated with gabaergic
18) Confidence 0.47 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.10
It has been reported that increased expression of snap-25 is associated with nonesterified fatty acid (NEFA)-induced impairment of insulin secretion in mouse islets [23].
Positive_regulation (increased) of Gene_expression (expression) of snap-25
19) Confidence 0.33 Published 2008 Journal Current Genomics Section Body Doc Link PMC2685644 Disease Relevance 0.58 Pain Relevance 0

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