INT252204

Context Info Confidence 0.70 First Reported 2008 Last Reported 2008 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 7 Disease Relevance 0.06 Pain Relevance 0.06

cell adhesion (GP9) plasma membrane (GP9)

Anatomy Link Frequency bridges 1 GP9 (Homo sapiens)

Pain Link Frequency Relevance Heat Pain 7 61.24

Disease Link Frequency Relevance Heat Bites And Stings 7 62.68 Aggression 7 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

To dephosphorylate GP-9B, an aliquot (50 µl) of the isolated protein sample (see above) was analyzed by LC-ESI/MS before and after incubation with 1 unit of alkaline phosphatase (Sigma, St.

Phosphorylation (dephosphorylate) of GP-9B

1) Confidence 0.70 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2582448 Disease Relevance 0 Pain Relevance 0

To identify a phosphorylated residue in GP-9B, an aliquot (200 µl) of the protein isolated by ion-exchange and gel filtration chromatography (see above) was dried in a SpeedVac and incubated with 25 µl of 8 M urea in 0.4 M ammonium bicarbonate, 2.3 µl of 100 mM DTT at 50°C for 15 min.

Phosphorylation (phosphorylated) of GP-9B

2) Confidence 0.61 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2582448 Disease Relevance 0 Pain Relevance 0

Thus, we conclude that in marked contrast to the hitherto known pheromone-binding proteins, GP-9B undergoes phosphorylation and thus existed in two forms, phosphorylated GP-9B (pGP-9B, observed MM 14,810 Da) and dephosphorylated form (GP-9B, observed MM 14,730 Da).

Phosphorylation (phosphorylated) of GP-9

3) Confidence 0.61 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2582448 Disease Relevance 0 Pain Relevance 0

GP-9B existed in two forms, one of them is phosphorylated.

Phosphorylation (phosphorylated) of GP-9B

4) Confidence 0.54 Published 2008 Journal PLoS ONE Section Abstract Doc Link PMC2582448 Disease Relevance 0.06 Pain Relevance 0.06

We then reasoned that the difference in the observed and calculated molecular masses could be due to phosphorylation of GP-9B, in addition to formation of disulfide bridges.

Phosphorylation (phosphorylation) of GP-9B in bridges

5) Confidence 0.54 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2582448 Disease Relevance 0 Pain Relevance 0

Thus, we conclude that in marked contrast to the hitherto known pheromone-binding proteins, GP-9B undergoes phosphorylation and thus existed in two forms, phosphorylated GP-9B (pGP-9B, observed MM 14,810 Da) and dephosphorylated form (GP-9B, observed MM 14,730 Da).

Phosphorylation (phosphorylated) of GP-9B

6) Confidence 0.54 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2582448 Disease Relevance 0 Pain Relevance 0

Thus, we conclude that in marked contrast to the hitherto known pheromone-binding proteins, GP-9B undergoes phosphorylation and thus existed in two forms, phosphorylated GP-9B (pGP-9B, observed MM 14,810 Da) and dephosphorylated form (GP-9B, observed MM 14,730 Da).

Phosphorylation (phosphorylation) of GP-9B

7) Confidence 0.54 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2582448 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.