INT258096

Context Info Confidence 0.36 First Reported 2009 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 5 Total Number 6 Disease Relevance 3.20 Pain Relevance 0.18

endoplasmic reticulum (Hcrt) cytoplasm (Hcrt)

Anatomy Link Frequency neural 1 Hcrt (Mus musculus)

Pain Link Frequency Relevance Heat nMDA receptor 4 95.08 Locus ceruleus 52 78.24 amygdala 8 69.88 gABA 32 5.00 Neuropeptide 16 5.00 Dopamine 10 5.00 medulla 10 5.00 anesthesia 6 5.00 Hippocampus 6 5.00 cocaine 4 5.00

Disease Link Frequency Relevance Heat Death 40 99.90 Targeted Disruption 202 99.60 Injury 4 95.72 Sarcoma 2 89.56 Sleep Disorders 356 89.36 Apoptosis 16 84.40 Cancer 12 58.72 Neuroblastoma 8 56.08 Malignant Neoplastic Disease 4 55.48 Sprains And Strains 50 50.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Previously, we demonstrated that the neurotoxin binds to HCRT receptors [6].

HCRT Binding (binds) of

1) Confidence 0.36 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2709920 Disease Relevance 0.34 Pain Relevance 0.07

Fluorescent activated cell sorting (FACS) analysis was used and determined that, indeed, HCRT2-SAP bound to the HCRT receptor but not the substance-P receptor.

HCRT receptor Binding (bound) of

2) Confidence 0.35 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2709920 Disease Relevance 0.29 Pain Relevance 0.06

IGFBP3 reduces promotor activity of hypocretin in neural cell lines

hypocretin Binding (activity) of in neural

3) Confidence 0.34 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2617764 Disease Relevance 0.48 Pain Relevance 0

Hypocretin cell counts were nonetheless normal in the hIGFBP3 transgenic model (3269±99 in HZ versus3230±140 in WT, n?

Hypocretin Binding (counts) of associated with targeted disruption

4) Confidence 0.29 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2617764 Disease Relevance 1.05 Pain Relevance 0

Hypocretin-1 radioimmunoassay

Hypocretin-1 Binding (radioimmunoassay) of

5) Confidence 0.29 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2617764 Disease Relevance 0 Pain Relevance 0

To test the hypothesis that excessive IGFBP3 in hypocretin cells contributes to this cell death, we crossed Hcrt-ataxin-3 transgenic mice (animals with targeted cell death 2–4 weeks after birth) with transgenic mice overexpressing human IGFBP3 (hIGFBP3), but found no effects on the speed of hypocretin cell death.

Hcrt Binding (crossed) of associated with targeted disruption and death

6) Confidence 0.29 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2617764 Disease Relevance 1.04 Pain Relevance 0.05

General Comments

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