INT260174

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Context Info
Confidence 0.78
First Reported 2009
Last Reported 2009
Negated 1
Speculated 0
Reported most in Body
Documents 29
Total Number 29
Disease Relevance 4.20
Pain Relevance 0.19

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
eyes 17
retina 1
teeth 1
Rpe65 (Mus musculus)
Pain Link Frequency Relevance Heat
abdominal pain 3 97.88 Very High Very High Very High
Bile 4 97.28 Very High Very High Very High
Central nervous system 3 96.68 Very High Very High Very High
Inflammation 84 28.28 Quite Low
ketamine 28 5.00 Very Low Very Low Very Low
anesthesia 28 5.00 Very Low Very Low Very Low
dexamethasone 28 5.00 Very Low Very Low Very Low
cva 28 5.00 Very Low Very Low Very Low
imagery 5 5.00 Very Low Very Low Very Low
COX-2 inhibitor 2 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Osteoma 7 100.00 Very High Very High Very High
Congenital Anomalies 5 100.00 Very High Very High Very High
Hypertrophy 4 100.00 Very High Very High Very High
Dentigerous Cyst 2 100.00 Very High Very High Very High
Odontoma 2 100.00 Very High Very High Very High
Supernumerary Tooth 2 100.00 Very High Very High Very High
Unerupted Tooth 2 100.00 Very High Very High Very High
Familial Adenomatous Polyposis 240 99.90 Very High Very High Very High
Desmoid Disease 24 99.60 Very High Very High Very High
Weight Loss 2 99.60 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Stable RPE65 expression throughout the whole retina was observed in the rd12 eyes five months after the subretinal injection of scAAV5-smCBA-hRPE65 (Figure 2A).
Gene_expression (expression) of RPE65 in eyes
1) Confidence 0.78 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0.07 Pain Relevance 0
RPE65 expression following intravitreal and subretinal injections of scAAV5-smCBA-hRPE65 vector into rd12 mice
Gene_expression (expression) of RPE65
2) Confidence 0.78 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0.07 Pain Relevance 0
In the contralateral eyes, which received an intravitreal injection with the same vector four weeks before the subretinal injection, little RPE65 expression could be detected (Figure 2B) except modest expression around the injection site (data not shown).
Gene_expression (expression) of RPE65 in eyes
3) Confidence 0.78 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0.06 Pain Relevance 0
In the partner rd12 eye that received an intravitreal injection, no obvious RPE65 expression was detected (Figure 2D).
Gene_expression (expression) of RPE65 in eye
4) Confidence 0.78 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
RPE65 expression following intravitreal and subretinal injections of scAAV5-smCBA-hRPE65 vector into rd12 mice
Gene_expression (expression) of scAAV5-smCBA-hRPE65
5) Confidence 0.68 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0.07 Pain Relevance 0
RPE65 expression following intravitreal and subretinal injections of scAAV5-smCBA-hRPE65 vector into rd12 mice
Gene_expression (expression) of rd12
6) Confidence 0.68 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0.07 Pain Relevance 0
Recently, a naturally occurring mouse model of human LCA with RPE65 mutations, the rd12 mouse, was reported [25].
Gene_expression (mutations) of RPE65
7) Confidence 0.68 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
The maximum b-wave amplitudes (Figure 4) in subretinally treated rd12 eyes were about 55% of those in the normal, uninjected C57BL/6J eyes.
Gene_expression (eyes) of rd12 in eyes
8) Confidence 0.68 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
Statistical analysis (Figure 4) showed that rd12 eyes that received either a second subretinal injection (n=3, p<0.001) or a single subretinal injection (n=3, p=0.001) had significantly higher b-wave amplitudes than the untreated rd12 eyes.
Gene_expression (eyes) of rd12 in eyes
9) Confidence 0.68 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
m thick frozen sections mounted on coated slides were rinsed with dPBS (Mediatech, Inc., Herndon, VA), blocked with 20% normal goat serum (NGS), incubated overnight at 4 °C in rabbit polyclonal raised anti-human/bovine RPE65 antibodies (Abcam, Cambridge, UK), and diluted 1:400 in NGS.
Gene_expression (antibodies) of RPE65
10) Confidence 0.68 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
The rd12 eye, which received a subretinal injection four weeks after an intravitreal injection in the contralateral eye of the same mouse (Figure 3C), showed robust, dark-adapted ERG restoration five months after the subretinal injection.
Gene_expression (eye) of rd12 in eye
11) Confidence 0.68 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
High magnification fluorescence microscopy (Figure 2C) showed that RPE65 was mainly located in the RPE cells of the rd12 eye following the subretinal injection of scAAV5-smCBA-hRPE65.
Gene_expression (located) of RPE65 in eye
12) Confidence 0.68 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0.05 Pain Relevance 0
AAV-mediated GFP expression in C57BL/6J mice and RPE65 expression and ERG restoration in rd12 mice were evaluated five months after the second subretinal injection.
Gene_expression (expression) of RPE65
13) Confidence 0.60 Published 2009 Journal Molecular Vision Section Abstract Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
Because of undetectable levels of RPE65, 11-cis-retinal, and rhodopsin at any age, no normal, dark-adapted electroretinogram (ERG) was detected in rd12 mice and slow rod degeneration ensued [25].
Gene_expression (levels) of RPE65
14) Confidence 0.60 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
Immunohistochemical data reported here suggest that the AAV5-mediated delivery of human RPE65 results in sustained human RPE65 protein expression in the rd12 retina for at least five months that correlates with a sustained restoration of dark-adapted ERG signals.
Gene_expression (expression) of RPE65 protein in retina
15) Confidence 0.60 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
RPE65 expression and ERG restoration has been detected as early as four weeks following subretinal injection (data not shown) and remained stable for at least five months after subretinal injection, the longest time we observed in this study.
Gene_expression (expression) of RPE65
16) Confidence 0.60 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
In this model, a recessive nonsense mutation in Rpe65 leads to the absence of the RPE65 protein and blockage of the retinoid cycle, which is essential for rod function.
Neg (absence) Gene_expression (absence) of RPE65 protein
17) Confidence 0.60 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
Immunostaining of retinal sections with anti-RPE65 antibody showed strong RPE65 expression mainly in the RPE cells of subretinally injected eyes but not in the intravitreally injected eyes except minimally around the injection site.


Gene_expression (expression) of RPE65 in eyes
18) Confidence 0.60 Published 2009 Journal Molecular Vision Section Abstract Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
Because of undetectable levels of RPE65, 11-cis-retinal, and rhodopsin at any age, no normal, dark-adapted electroretinogram (ERG) was detected in rd12 mice and slow rod degeneration ensued [25].
Gene_expression (undetectable) of RPE65
19) Confidence 0.60 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0
Statistical analysis (Figure 4) showed that rd12 eyes that received either a second subretinal injection (n=3, p<0.001) or a single subretinal injection (n=3, p=0.001) had significantly higher b-wave amplitudes than the untreated rd12 eyes.
Gene_expression (eyes) of rd12 in eyes
20) Confidence 0.59 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2633462 Disease Relevance 0 Pain Relevance 0

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