INT261058

Context Info Confidence 0.04 First Reported 2009 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 5 Disease Relevance 0.14 Pain Relevance 0.38

nucleoplasm (CALM3) small molecule metabolic process (CALM3) carbohydrate metabolic process (CALM3) cytoplasm (CALM3) cytosol (CALM3) signal transduction (CALM3)

CALM3 (Homo sapiens) CHR (Homo sapiens)

Pain Link Frequency Relevance Heat Calcium channel 85 88.00 antagonist 5 49.36 Inflammatory response 5 15.44 Inflammation 50 5.00 Catecholamine 30 5.00 imagery 20 5.00 medulla 20 5.00 Inflammatory stimuli 10 5.00 cytokine 5 5.00 Pain 5 5.00

Disease Link Frequency Relevance Heat Death 5 81.92 Infection 10 54.76 INFLAMMATION 65 15.04 Apoptosis 10 5.00 Shock 10 5.00 Pain 5 5.00 Fungal Infection 5 5.00 Disease 5 5.00 Stress 5 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

In addition, CaM interacts with both CHR and CAT (Figure 5C2) when assayed by CaM-binding affinity chromatography and dot blot immunodetection of CgA in the fixed material with anti-CHR (monoclonal anti-CGA47–66) [14] and anti-CAT (polyclonal anti-CGA344–364) [17].

CaM Binding (interacts) of CHR

1) Confidence 0.04 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2639705 Disease Relevance 0 Pain Relevance 0.09

Interactions between CaM, CAT and CHR were assessed by surface plasmon resonance (SPR) (Figure 5 C1), revealing that not only CHR but also CAT specifically interacted with CaM, while scrambled CAT was inactive.

CaM Binding (interacted) of CHR

2) Confidence 0.04 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2639705 Disease Relevance 0 Pain Relevance 0.09

Interestingly, a comparison of CHR and CAT sequences with two CaM-binding peptides of iPLA2 reveals marked homologies (Figure 6D).

CaM Binding (comparison) of CHR

3) Confidence 0.03 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2639705 Disease Relevance 0 Pain Relevance 0.11

The present experiments have demonstrated that the two CgA peptides, CHR and CAT, penetrate rapidly into PMNs (Figure 5A) and subsequently interact with CaM (Figure 5C) to activate iPLA2 (Figure 6C).

CaM Binding (interact) of CHR

4) Confidence 0.03 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2639705 Disease Relevance 0.14 Pain Relevance 0.04

The two cationic sequences CAT and CHR may be aligned with two Ca2+- dependent CaM binding motifs 1-8-14 B and 1-5-10 [39], as illustrated in Figure 5 B and previously demonstrated for binding of CHR to CaM [24], [40].

CaM Binding (binding) of CHR

5) Confidence 0.03 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2639705 Disease Relevance 0 Pain Relevance 0.03

General Comments

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