INT261058
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
In addition, CaM interacts with both CHR and CAT (Figure 5C2) when assayed by CaM-binding affinity chromatography and dot blot immunodetection of CgA in the fixed material with anti-CHR (monoclonal anti-CGA4766) [14] and anti-CAT (polyclonal anti-CGA344364) [17].
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Interactions between CaM, CAT and CHR were assessed by surface plasmon resonance (SPR) (Figure 5 C1), revealing that not only CHR but also CAT specifically interacted with CaM, while scrambled CAT was inactive. | |||||||||||||||
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Interestingly, a comparison of CHR and CAT sequences with two CaM-binding peptides of iPLA2 reveals marked homologies (Figure 6D). | |||||||||||||||
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The present experiments have demonstrated that the two CgA peptides, CHR and CAT, penetrate rapidly into PMNs (Figure 5A) and subsequently interact with CaM (Figure 5C) to activate iPLA2 (Figure 6C). | |||||||||||||||
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The two cationic sequences CAT and CHR may be aligned with two Ca2+- dependent CaM binding motifs 1-8-14 B and 1-5-10 [39], as illustrated in Figure 5 B and previously demonstrated for binding of CHR to CaM [24], [40]. | |||||||||||||||
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General Comments
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