INT261190

Context Info Confidence 0.60 First Reported 2009 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 3 Total Number 3 Disease Relevance 0 Pain Relevance 0

cytosol (Hprt) transferase activity, transferring glycosyl groups (Hprt) cytoplasm (Hprt)

Anatomy Link Frequency internal 1 Hprt (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 3 5.00 imagery 3 5.00 cerebral cortex 2 5.00 Pain 2 5.00 cytokine 1 5.00 anesthesia 1 5.00 Inflammatory response 1 5.00 Osteoarthritis 1 5.00 isoflurane 1 5.00

Disease Link Frequency Relevance Heat Obesity 10 11.76 Osteogenic Sarcomas 4 6.32 Diabetes Mellitus 10 5.00 Cancer 7 5.00 Neurodegenerative Disease 7 5.00 Disease 7 5.00 Microcephaly 6 5.00 Syndrome 6 5.00 Chromosome Breakage 5 5.00 Osteoporosis 4 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Briefly, the signal of the endogenous HPRT1 control was used to normalize the target gene signals of each sample.

Regulation (control) of HPRT1

1) Confidence 0.60 Published 2009 Journal The Open Orthopaedics Journal Section Body Doc Link PMC2761671 Disease Relevance 0 Pain Relevance 0

HPRT was used as an internal control in the PCR reaction.

Regulation (control) of HPRT in internal

2) Confidence 0.19 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2642846 Disease Relevance 0 Pain Relevance 0

The respective Ct-values of the Hprt, and Mcph1 wild type and trap alleles were related to the standard curves.

Regulation (values) of Hprt

3) Confidence 0.18 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2821930 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.