INT263675

Context Info Confidence 0.75 First Reported 2009 Last Reported 2009 Negated 1 Speculated 0 Reported most in Body Documents 1 Total Number 7 Disease Relevance 0.45 Pain Relevance 0.56

plasma membrane (Neto1)

Anatomy Link Frequency brain 3 tail 1 spines 1 Neto1 (Mus musculus)

Pain Link Frequency Relevance Heat Hippocampus 84 97.12 cerebral cortex 7 95.12 Central nervous system 21 92.24 long-term potentiation 182 62.76 Glutamate receptor 14 44.08 Neurotransmitter 21 18.84 nMDA receptor 63 5.00 Pyramidal cell 28 5.00 Eae 14 5.00 Glutamate 14 5.00

Disease Link Frequency Relevance Heat Cognitive Disorder 56 96.60 Congenital Anomalies 42 94.80 Contagious Ecthyma 7 81.92 Convulsion 14 24.00 Sprains And Strains 7 5.00 Helminth Infection 7 5.00 Influenza Virus Infection 7 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Furthermore, using crude synaptosomal fractions, we determined that anti-PSD-95 antibodies co-immunoprecipitated Neto1 from wild-type (Neto1+/+) mouse brain (Figure 2E).

Localization (immunoprecipitated) of Neto1 in brain

1) Confidence 0.75 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0.07

The absence of Neto1 had no effect on the overall abundance of NR1, NR2A, NR2B, PSD-95, GluR2, VAMP2, or GABAAR1 proteins (Figure 6L) in whole brain extracts, or of NR1, NR2A, NR2B, or PSD-95 in crude synaptosomes (Figure 6M).

Localization (absence) of Neto1 in brain

2) Confidence 0.75 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0.18 Pain Relevance 0.05

Negative control antibodies did not immunoprecipitate either Neto1 or PSD-95 (Figures 2E and 3A).

Neg (not) Localization (immunoprecipitate) of Neto1

3) Confidence 0.75 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0.06

We also found that Neto1 co-localized with actin (Figure 2D), which is highly enriched in dendritic spines in the hippocampus [20].

Localization (localized) of Neto1 in spines associated with hippocampus

4) Confidence 0.75 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0.19

Removal of the cytoplasmic tail and transmembrane domain of Neto1 did not abolish the Neto1:NMDAR interaction (Figure 4B, lanes 2 and 3), suggesting that it was mediated by the ectodomain of Neto1.

Localization (domain) of Neto1 in tail

5) Confidence 0.75 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0

To identify the subcellular compartments in which Neto1 is localized, we performed subcellular fractionation and immunoblotting experiments of whole mouse brain lysates.

Localization (localized) of Neto1 in brain

6) Confidence 0.75 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0.20

Neto1 cloning and gene targeting.

Localization (targeting) of Neto1

7) Confidence 0.65 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0.27 Pain Relevance 0

General Comments

This test has worked.