INT263683

Context Info Confidence 0.59 First Reported 2009 Last Reported 2009 Negated 1 Speculated 0 Reported most in Body Documents 1 Total Number 9 Disease Relevance 0.36 Pain Relevance 0.61

plasma membrane (Neto1)

Anatomy Link Frequency tail 2 stem 1 hippocampus 1 Neto1 (Mus musculus)

Pain Link Frequency Relevance Heat long-term potentiation 234 98.76 Hippocampus 108 96.52 Neurotransmitter 27 94.04 Eae 18 74.56 Glutamate receptor 18 43.12 addiction 9 33.32 nMDA receptor 81 5.00 Pyramidal cell 36 5.00 Central nervous system 27 5.00 Glutamate 18 5.00

Disease Link Frequency Relevance Heat Congenital Anomalies 54 91.76 Cognitive Disorder 72 74.08 Convulsion 18 5.00 Sprains And Strains 9 5.00 Helminth Infection 9 5.00 Influenza Virus Infection 9 5.00 Contagious Ecthyma 9 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

The membrane domains of NR2A and NR2B, however, are over 95% identical and are therefore unlikely to be responsible for the differential effect of loss of Neto1.

Negative_regulation (loss) of Neto1

1) Confidence 0.59 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0

Altogether, the above findings establish that Neto1-null mice are impaired in hippocampal-dependent spatial learning.

Negative_regulation (impaired) of Neto1-null

2) Confidence 0.59 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0.22 Pain Relevance 0.04

The loss of Neto1, while having no effect on basal AMPAR-mediated synaptic transmission, suppresses LTP to a degree comparable to that observed in mice lacking NR2A [52] or its C-terminal tail [53].

Negative_regulation (loss) of Neto1 in tail associated with long-term potentiation

3) Confidence 0.59 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0.09

We have established that Neto1 is a critical component of the NMDAR complex, and that loss of Neto1 leads to impaired hippocampal LTP and hippocampal-dependent learning and memory.

Negative_regulation (loss) of Neto1 associated with long-term potentiation

4) Confidence 0.59 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0.17

The preferential effect of the loss of Neto1 on the abundance of synaptic, but not total, NR2A-containing NMDARs would not have been predicted from studies on the basis of the disruption of other NMDAR-interacting proteins.

Negative_regulation (loss) of Neto1

5) Confidence 0.59 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0.06

Rather, loss of Neto1 and LEV-10 each leads to a reduction in synaptic localization of the cognate receptors, whereas loss of SOL-1 leads to a loss of function of normally distributed GLR-1.

Negative_regulation (loss) of Neto1

6) Confidence 0.59 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0.11

Although Neto1 binds to both NR2A and NR2B, the loss of Neto1 leads to a reduction in the abundance of NR2A, but not NR2B, in the PSD fraction from hippocampus and a reduction in NR2A puncta in the CA1 region.

Negative_regulation (loss) of Neto1 in hippocampus associated with hippocampus

7) Confidence 0.59 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0.12

Removal of the cytoplasmic tail and transmembrane domain of Neto1 did not abolish the Neto1:NMDAR interaction (Figure 4B, lanes 2 and 3), suggesting that it was mediated by the ectodomain of Neto1.

Neg (not) Negative_regulation (abolish) of Neto1 in tail

8) Confidence 0.43 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0

To determine whether Neto1 is required for normal brain function in the mouse, we disrupted the Neto1 locus by homologous recombination in mouse embryonic stem (ES) cells.

Negative_regulation (disrupted) of Neto1 in stem

9) Confidence 0.43 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0.14 Pain Relevance 0.03

General Comments

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