INT264709

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Context Info
Confidence 0.76
First Reported 2008
Last Reported 2010
Negated 1
Speculated 1
Reported most in Body
Documents 24
Total Number 25
Disease Relevance 7.04
Pain Relevance 0.63

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell adhesion (Hes1) nucleus (Hes1) intracellular (Hes1)
protein complex assembly (Hes1) DNA binding (Hes1) transcription factor binding (Hes1)
Anatomy Link Frequency
Daoy 3
cerebellar granule cells 1
Notch 1
ventricular zone 1
neural 1
Hes1 (Mus musculus)
Pain Link Frequency Relevance Heat
imagery 280 96.28 Very High Very High Very High
Central nervous system 24 64.72 Quite High
cytokine 1 61.80 Quite High
metalloproteinase 1 57.04 Quite High
ketamine 25 5.00 Very Low Very Low Very Low
anesthesia 24 5.00 Very Low Very Low Very Low
alcohol 23 5.00 Very Low Very Low Very Low
ischemia 2 5.00 Very Low Very Low Very Low
Pain 1 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Cancer 1727 99.20 Very High Very High Very High
Neurodegenerative Disease 69 89.72 High High
Medulloblastoma 161 80.56 Quite High
Toxicity 23 78.80 Quite High
Apoptosis 115 77.56 Quite High
Severe Combined Immunodeficiency 23 69.40 Quite High
Recurrence 23 68.16 Quite High
Death 24 67.60 Quite High
Repression 23 67.12 Quite High
Central Nervous System Cancer 23 64.96 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
With this scenario, miR-199b-5p can be seen as part of the complex Notch signal-transduction pathway, as a fine tuner of the levels of expression of the HES1 bHLH transcription factor.
Gene_expression (expression) of HES1 in Notch
1) Confidence 0.76 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.09 Pain Relevance 0
When full-length HES1 cDNA was cloned and transfected into the stable Daoy-cell 199bSC1 clone, HES1 expression was restored, as evaluated by immunoblotting (Fig. 3A).
Gene_expression (expression) of HES1 in Daoy
2) Confidence 0.76 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.32 Pain Relevance 0
Furthermore, the transient transfection of HES1 cDNA into the stable 199bSC1 clone led to an increase in the percentage of cells in S phase, and a removal of the block on the G0-G1 phase (Fig. 3D).
Gene_expression (transfection) of HES1 cDNA
3) Confidence 0.76 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.06 Pain Relevance 0
At the same time, the transfection of HES1 cDNA into the 199bSC1 clone decreased induction of pro-neural bHLHs and differentiation markers, and increased levels of proliferation genes (Fig. 3C).
Gene_expression (transfection) of HES1 cDNA in neural
4) Confidence 0.76 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.18 Pain Relevance 0
When full-length HES1 cDNA was cloned and transfected into the stable Daoy-cell 199bSC1 clone, HES1 expression was restored, as evaluated by immunoblotting (Fig. 3A).
Gene_expression (transfected) of HES1 cDNA in Daoy
5) Confidence 0.76 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.39 Pain Relevance 0.03
Down-regulation of HES1 expression by miR-199b-5p negatively regulates the proliferation rate and anchorage-independent growth of MB cells.
Gene_expression (expression) of HES1
6) Confidence 0.76 Published 2009 Journal PLoS ONE Section Abstract Doc Link PMC2656623 Disease Relevance 0.81 Pain Relevance 0
Restored HES1 expression also reversed the effects of miR-199b-5p on cell proliferation (Fig. 3B).
Gene_expression (expression) of HES1
7) Confidence 0.76 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.19 Pain Relevance 0
Indeed, it has already been shown that HES1 protein levels correlate with negative outcome in MB patients [24].
Gene_expression (levels) of HES1
8) Confidence 0.66 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.32 Pain Relevance 0
Hsa-miR-199b-5p silences HES1 expression via 3?
Gene_expression (expression) of HES1
9) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.55 Pain Relevance 0
We report here a novel level of regulation of HES1 expression that is driven by miR-199b-5p, which binds to the HES1 3?
Gene_expression (expression) of HES1
10) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.07 Pain Relevance 0
The effects of restored expression of HES1 on the SP of the stable 199bSC1 clone were also evaluated.
Gene_expression (expression) of HES1
11) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.05 Pain Relevance 0
Significantly, expression of HES1 in MB has been associated with worse clinical outcome [24].
Gene_expression (expression) of HES1
12) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.51 Pain Relevance 0
Overall, we assessed the levels of persistence of adenovirus expression in infected cells, as the down-regulation of HES1 expression due to miR199b carrying the adenovirus expression, thus following tumor growth over time by BLI see Figure 4G–H, and antibodies staining Figure S4L–M (Hes1, Ki67, Gabra6, Nestin, Math-3, see details in Text S1 information).
Gene_expression (expression) of HES1 associated with cancer and imagery
13) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.36 Pain Relevance 0.18
Three clones demonstrated reduced HES1 protein levels, one of which (199bSC1) showed no detectable HES1.
Neg (no) Gene_expression (detectable) of HES1
14) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.06 Pain Relevance 0
The clones obtained were tested for luciferase expression levels and also validated for retention of the parental phenotype, in terms of both HES1 and miR-199b-5p expression (see Text S1, Fig.
Gene_expression (expression) of HES1
15) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.48 Pain Relevance 0.08
These effects of miR-199b-5p on HES1 protein expression were not restricted to the stable clones or Daoy cells, as D283MED cells transiently transfected with the expression construct for miR-199b-5p also showed reduced HES1 levels (Text S1, Fig.
Gene_expression (expression) of HES1 in Daoy
16) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.07 Pain Relevance 0
HES1 over-expression rescues the miR-199b-5p clone phenotype
Gene_expression (expression) of HES1
17) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.46 Pain Relevance 0.03
Transfection of this antisense 2-OM induced a marked increase in proliferation of the stable 199bSC1 clone, in agreement with restored expression levels of HES1.
Gene_expression (expression) of HES1
18) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0 Pain Relevance 0
The xenograft tumors from mouse #5 and mouse #4 were explanted and analyzed for miR-199b-5p and HES1 expression and evaluated histopathologically (Text S1, Fig.
Spec (analyzed) Gene_expression (expression) of HES1 associated with cancer
19) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.63 Pain Relevance 0.10
This is probably due to the short time of re-expression of HES1 after transient transfection, which might not be enough to allow for complete phenotype rescue.
Gene_expression (expression) of HES1
20) Confidence 0.59 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.12 Pain Relevance 0

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