INT265513

Context Info Confidence 0.48 First Reported 2009 Last Reported 2009 Negated 1 Speculated 1 Reported most in Body Documents 2 Total Number 26 Disease Relevance 5.75 Pain Relevance 0

mitochondrion (Opa1) mitochondrion organization (Opa1) GTPase activity (Opa1)

Anatomy Link Frequency vesicles 1 Opa1 (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 27 5.00 pain pelvic 25 5.00 Pain 2 5.00 cva 2 5.00 imagery 1 5.00 alcohol 1 5.00 headache 1 5.00

Disease Link Frequency Relevance Heat Sprains And Strains 975 99.48 Neuroleptic Malignant Syndrome 225 99.18 Adhesions 25 98.52 Disease 36 93.68 Infection 450 85.92 Gonorrhea 325 79.72 Atrophy 7 71.44 Bardet-biedl Syndrome 150 69.92 Chlamydia Infection 125 63.52 Confusion 1 62.92

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

This domain is predicted to form a coiled-coil domain, allowing interactions between OPA1 proteins.

OPA1 Binding (interactions) of

1) Confidence 0.48 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2661005 Disease Relevance 0.39 Pain Relevance 0

When room temperature-treated samples were analyzed, AbSV linear recognized only OpaA, OpaK and OpaF.

OpaK Binding (recognized) of

2) Confidence 0.35 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.25 Pain Relevance 0

When room temperature-treated samples were analyzed, AbSV linear recognized only OpaA, OpaK and OpaF.

OpaF Binding (recognized) of

3) Confidence 0.35 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.25 Pain Relevance 0

12.5 compared to NMS (Figures 4C and D) although AbSV cyclic bound OpaK variants.

OpaK Binding (bound) of associated with neuroleptic malignant syndrome

4) Confidence 0.35 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.76 Pain Relevance 0

AbSV linear bound only OpaA, OpaK and OpaF variants (Figure 3B), which is consistent with western blot results against unheated lysates in which native conformations are preserved.

OpaK Binding (bound) of

5) Confidence 0.35 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.16 Pain Relevance 0

AbSV linear bound only OpaA, OpaK and OpaF variants (Figure 3B), which is consistent with western blot results against unheated lysates in which native conformations are preserved.

OpaF Binding (bound) of

6) Confidence 0.30 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.16 Pain Relevance 0

The resultant antiserum, AbSV cyclic, was more broadly reactive than AbSV linear and recognized all but 2 Opa proteins of strain FA1090 when denatured samples were analyzed (Figure 2B).

Opa Spec (analyzed) Binding (recognized) of associated with sprains and strains

7) Confidence 0.30 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.16 Pain Relevance 0

AbSV cyclic bound intact OpaA, OpaB, OpaD, OpaF, OpaK and OpaI variants above background in the SBI assay at a dilution of 1?

OpaI Binding (bound) of

8) Confidence 0.27 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.07 Pain Relevance 0

AbSV cyclic recognized OpaA, OpaK, OpaD and OpaI proteins when RT-treated samples were used.

OpaK Binding (recognized) of

9) Confidence 0.27 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.16 Pain Relevance 0

AbSV cyclic bound intact OpaA, OpaB, OpaD, OpaF, OpaK and OpaI variants above background in the SBI assay at a dilution of 1?

OpaK Binding (bound) of

10) Confidence 0.27 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.07 Pain Relevance 0

OpaD and OpaI proteins were not recognized.

OpaI Binding (recognized) of

11) Confidence 0.27 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.25 Pain Relevance 0

40, while OpaC, OpaE, and Opa-negative variants were not recognized by AbSV cyclic (Figure 3D).

OpaE Binding (recognized) of

12) Confidence 0.27 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.06 Pain Relevance 0

AbSV cyclic bound intact OpaA, OpaB, OpaD, OpaF, OpaK and OpaI variants above background in the SBI assay at a dilution of 1?

OpaF Binding (bound) of

13) Confidence 0.27 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.07 Pain Relevance 0

40, while OpaC, OpaE, and Opa-negative variants were not recognized by AbSV cyclic (Figure 3D).

Opa-negative Binding (recognized) of

14) Confidence 0.27 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.06 Pain Relevance 0

AbSV cyclic recognized OpaA, OpaK, OpaD and OpaI proteins when RT-treated samples were used.

OpaI Binding (recognized) of

15) Confidence 0.27 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.16 Pain Relevance 0

Consistent with the results obtained by SBI, AbSV linear (2.2 µg/mL) bound OpaA variants as well as OpaK and OpaF-expressing gonococci, but none of the other Opa variants.

OpaF Binding (bound) of

16) Confidence 0.26 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0 Pain Relevance 0

Ab4L linear strongly recognized all of the Opa proteins of strain FA1090 except OpaE (Figure 2C) as reported previously [30].

Opa Neg (except) Binding (recognized) of associated with sprains and strains

17) Confidence 0.26 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.38 Pain Relevance 0

In summary, we have demonstrated that broadly-reactive antibodies can be generated against a relatively conserved Opa protein loop that bind to the bacterial surface and have agglutination ability.

Opa Binding (bind) of

18) Confidence 0.26 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.38 Pain Relevance 0

We also tested AbSV cyclic, which may recognize conformational epitopes among Opa proteins compared to AbSV linear.

Opa Binding (recognize) of

19) Confidence 0.26 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.14 Pain Relevance 0

AbSV cyclic recognized Opa proteins with up to 7 amino acid differences compared to the cyclic SV peptide, but not OpaC or OpaE, which are the most divergent from OpaA/K in the SV region (Figure 1).

Opa Binding (recognized) of

20) Confidence 0.26 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2779592 Disease Relevance 0.21 Pain Relevance 0

General Comments

This test has worked.