INT26585
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
At the myoblast stage, cells expressed both vimentin and desmin. | |||||||||||||||
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Vimentin gene expression and homing of GAS were followed by real-time reverse-transcriptase polymerase chain reaction and quantitative bacteriology of muscle homogenates, respectively. | |||||||||||||||
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Muscle injury, vimentin expression, and nonsteroidal anti-inflammatory drugs predispose to cryptic group A streptococcal necrotizing infection. | |||||||||||||||
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In vitro the RM1 cells express E-cadherin but not vimentin, do not form colonies in soft agar, are non-invasive but are more motile than the parent cell line. | |||||||||||||||
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The uncommitted neural crest cells express a mesenchymal intermediate filament protein such as vimentin, but not the usual neuronal markers such as receptor sites for tetanus toxin or neurofilaments. | |||||||||||||||
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Vimentin could not be detected in all groups. | |||||||||||||||
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Figure 2L shows as an example the FACS-based quantitation of vimentin expression in G-2 cells at passage 20. | |||||||||||||||
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The co-expression of vimentin and cytokeratins in individual cells in high-grade WAP-T tumors and in G-2 cultures probably can also be attributed to the expansion of transformed, vimentin-expressing cells normally present in the mammary epithelial stem cell system. | |||||||||||||||
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In transplanted G-2 tumors (Figure 4A) and high-grade WAP-T tumors (Figure 4B) we observed in addition to the expected expression of vimentin in the stromal compartment also the co-expression of vimentin and SV40-LT in individual tumor cells. | |||||||||||||||
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Independent of passage number nearly all G-2 cells express vimentin, whereby the intensity of vimentin expression ranges from a diffuse cytoplasmic distribution and faint filamentous structures to an abundant filamentous network (Figure 2JK show vimentin/SV40-LT and vimentin/Krt18 co-staining at passage 10). | |||||||||||||||
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SV40-LT expression was detected almost always also in cells strongly expressing vimentin (Figure 2J). | |||||||||||||||
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Indeed, significant vimentin expression was detected in the MRU (mammary repopulating unit) cell population that is responsible for mammary tissue regeneration upon transplantation into the cleared fat pad [56]. | |||||||||||||||
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While in low-grade WAP-T tumors vimentin staining was mostly limited to septa and the stromal compartment (Figure 3C), variable expression of vimentin was also detectable in the epithelial compartment (represented by Krt8/18) in high-grade WAP-T tumors (Figure 3B), indicating an intermediate differentiation state of tumor cells in G-2 derived and high-grade WAP-T tumors and an epithelial differentiation state of tumor cells in low-grade WAP-T tumors. | |||||||||||||||
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Independent of passage number nearly all G-2 cells express vimentin, whereby the intensity of vimentin expression ranges from a diffuse cytoplasmic distribution and faint filamentous structures to an abundant filamentous network (Figure 2JK show vimentin/SV40-LT and vimentin/Krt18 co-staining at passage 10). | |||||||||||||||
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Since vimentin is expressed during mammary morphogenesis [57] and is associated with a motile phenotype [58], it is conceivable that vimentin marks a subset of non-constrained cells required for tissue maintenance. | |||||||||||||||
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Immunostaining analysis of vimentin and Krt8/18 expression in G-2 cell derived tumors (Figure 3A) revealed their close resemblance to poorly differentiated (grade G3) WAP-T tumors (Figure 3B). | |||||||||||||||
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Interestingly, the tumor cells were largely devoid of epithelial markers, Epcam (Figure S2A) and cytokeratins (Figure S2B, D), but strongly expressed vimentin (Figure S2B, C), indicating that in non-transgenic mice transition into the mesenchymal state is favored, possibly as a consequence of an interaction with the host immune system. | |||||||||||||||
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G-2 cultures comprise cell populations in distinct differentiation states identified by co-expression of cytoskeletal proteins (cytokeratins and vimentin), a combination of cell surface markers and a set of transcription factors. | |||||||||||||||
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Similar to the parental culture, heterogeneous expression of vimentin was observed in G-2 cell clones (Figure S1G). | |||||||||||||||
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In summary, by immunostaining analysis we observed firstly that the majority of G-2 cells are distinguished by a differentiation state characterized by co-expression of vimentin and cytokeratins, secondly that the number of cells devoid of cytokeratins fluctuates between passages, and thirdly that a minor population of cells expressing cytokeratins but totally lacking vimentin is always present. | |||||||||||||||
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