INT26585

Context Info Confidence 0.77 First Reported 1988 Last Reported 2011 Negated 8 Speculated 1 Reported most in Body Documents 117 Total Number 120 Disease Relevance 39.97 Pain Relevance 6.00

cytosol (Vim) structural molecule activity (Vim) cytoskeleton (Vim) cytoplasm (Vim)

Anatomy Link Frequency radial 19 nerves 4 neurons 4 radial glial cells 3 Muscle 3 Vim (Mus musculus)

Pain Link Frequency Relevance Heat dorsal root ganglion 287 100.00 Dismenorea 26 99.80 cINOD 9 99.76 Sciatic nerve 437 98.88 cerebral cortex 160 98.64 Peripheral nerve injury 6 97.00 Inflammation 154 93.80 Somatosensory cortex 128 90.28 Glutamate 2 84.16 palliative 2 80.72

Disease Link Frequency Relevance Heat Ganglion Cysts 340 100.00 Malignant Neoplastic Disease 83 100.00 Retina Disease 72 100.00 Renal Cell Carcinoma 26 99.98 Injury 377 99.96 Dysmenorrhea 26 99.80 Cancer 4417 99.74 Neuroma 237 99.68 Disseminated Intravascular Coagulation 320 99.40 INFLAMMATION 156 99.04

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

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Disease term

At the myoblast stage, cells expressed both vimentin and desmin.

Gene_expression (expressed) of vimentin in myoblast

1) Confidence 0.77 Published 1988 Journal Dev. Biol. Section Abstract Doc Link 3044880 Disease Relevance 0.31 Pain Relevance 0.29

Vimentin gene expression and homing of GAS were followed by real-time reverse-transcriptase polymerase chain reaction and quantitative bacteriology of muscle homogenates, respectively.

Gene_expression (expression) of Vimentin in muscle

2) Confidence 0.76 Published 2008 Journal J. Infect. Dis. Section Body Doc Link 18939933 Disease Relevance 0 Pain Relevance 0

Muscle injury, vimentin expression, and nonsteroidal anti-inflammatory drugs predispose to cryptic group A streptococcal necrotizing infection.

Gene_expression (expression) of vimentin in Muscle associated with inflammation, injury, cinod and infection

3) Confidence 0.76 Published 2008 Journal J. Infect. Dis. Section Title Doc Link 18939933 Disease Relevance 0.66 Pain Relevance 0.36

In vitro the RM1 cells express E-cadherin but not vimentin, do not form colonies in soft agar, are non-invasive but are more motile than the parent cell line.

Neg (not) Gene_expression (express) of vimentin

4) Confidence 0.75 Published 2009 Journal Prostate Section Body Doc Link 19585491 Disease Relevance 0.14 Pain Relevance 0

The uncommitted neural crest cells express a mesenchymal intermediate filament protein such as vimentin, but not the usual neuronal markers such as receptor sites for tetanus toxin or neurofilaments.

Neg (not) Gene_expression (express) of vimentin in neuronal associated with anaerobic bacterial infections

5) Confidence 0.64 Published 1989 Journal Development Section Abstract Doc Link 2562776 Disease Relevance 0.19 Pain Relevance 0.12

Vimentin could not be detected in all groups.

Neg (not) Gene_expression (detected) of Vimentin

6) Confidence 0.64 Published 2009 Journal Reproduction Section Abstract Doc Link 19451194 Disease Relevance 0.16 Pain Relevance 0.24

Figure 2L shows as an example the FACS-based quantitation of vimentin expression in G-2 cells at passage 20.

Gene_expression (expression) of vimentin

7) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 0.08 Pain Relevance 0

The co-expression of vimentin and cytokeratins in individual cells in high-grade WAP-T tumors and in G-2 cultures probably can also be attributed to the expansion of transformed, vimentin-expressing cells normally present in the mammary epithelial “stem cell system”.

Gene_expression (expression) of vimentin in stem cell associated with cancer

8) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 0.38 Pain Relevance 0

In transplanted G-2 tumors (Figure 4A) and high-grade WAP-T tumors (Figure 4B) we observed in addition to the expected expression of vimentin in the stromal compartment also the co-expression of vimentin and SV40-LT in individual tumor cells.

Gene_expression (expression) of vimentin associated with cancer

9) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 1.61 Pain Relevance 0

Independent of passage number nearly all G-2 cells express vimentin, whereby the intensity of vimentin expression ranges from a diffuse cytoplasmic distribution and faint filamentous structures to an abundant filamentous network (Figure 2J–K show vimentin/SV40-LT and vimentin/Krt18 co-staining at passage 10).

Gene_expression (expression) of vimentin

10) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 0.09 Pain Relevance 0

SV40-LT expression was detected almost always also in cells strongly expressing vimentin (Figure 2J).

Gene_expression (expressing) of vimentin

11) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 0.08 Pain Relevance 0

Indeed, significant vimentin expression was detected in the MRU (mammary repopulating unit) cell population that is responsible for mammary tissue regeneration upon transplantation into the cleared fat pad [56].

Gene_expression (expression) of vimentin in fat pad associated with peripheral arterial disease

12) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 0.38 Pain Relevance 0

While in low-grade WAP-T tumors vimentin staining was mostly limited to septa and the stromal compartment (Figure 3C), variable expression of vimentin was also detectable in the epithelial compartment (represented by Krt8/18) in high-grade WAP-T tumors (Figure 3B), indicating an intermediate differentiation state of tumor cells in G-2 derived and high-grade WAP-T tumors and an epithelial differentiation state of tumor cells in low-grade WAP-T tumors.

Gene_expression (expression) of vimentin associated with cancer

13) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 1.34 Pain Relevance 0

Independent of passage number nearly all G-2 cells express vimentin, whereby the intensity of vimentin expression ranges from a diffuse cytoplasmic distribution and faint filamentous structures to an abundant filamentous network (Figure 2J–K show vimentin/SV40-LT and vimentin/Krt18 co-staining at passage 10).

Gene_expression (express) of vimentin

14) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 0.09 Pain Relevance 0

Since vimentin is expressed during mammary morphogenesis [57] and is associated with a motile phenotype [58], it is conceivable that vimentin marks a subset of non-constrained cells required for tissue maintenance.

Gene_expression (expressed) of vimentin

15) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 0.48 Pain Relevance 0

Immunostaining analysis of vimentin and Krt8/18 expression in G-2 cell derived tumors (Figure 3A) revealed their close resemblance to poorly differentiated (grade G3) WAP-T tumors (Figure 3B).

Gene_expression (expression) of vimentin associated with cancer

16) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 0.93 Pain Relevance 0

Interestingly, the tumor cells were largely devoid of epithelial markers, Epcam (Figure S2A) and cytokeratins (Figure S2B, D), but strongly expressed vimentin (Figure S2B, C), indicating that in non-transgenic mice transition into the mesenchymal state is favored, possibly as a consequence of an interaction with the host immune system.

Gene_expression (expressed) of vimentin in immune system associated with targeted disruption and cancer

17) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 1.34 Pain Relevance 0

G-2 cultures comprise cell populations in distinct differentiation states identified by co-expression of cytoskeletal proteins (cytokeratins and vimentin), a combination of cell surface markers and a set of transcription factors.

Gene_expression (expression) of vimentin

18) Confidence 0.64 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2920333 Disease Relevance 0.69 Pain Relevance 0

Similar to the parental culture, heterogeneous expression of vimentin was observed in G-2 cell clones (Figure S1G).

Gene_expression (expression) of vimentin

19) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 0.28 Pain Relevance 0

In summary, by immunostaining analysis we observed firstly that the majority of G-2 cells are distinguished by a differentiation state characterized by co-expression of vimentin and cytokeratins, secondly that the number of cells devoid of cytokeratins fluctuates between passages, and thirdly that a minor population of cells expressing cytokeratins but totally lacking vimentin is always present.

Gene_expression (expression) of vimentin

20) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920333 Disease Relevance 0.14 Pain Relevance 0

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