INT26891

Context Info Confidence 0.74 First Reported 1989 Last Reported 2010 Negated 0 Speculated 4 Reported most in Body Documents 21 Total Number 25 Disease Relevance 2.08 Pain Relevance 3.64

nucleoplasm (OGG1) mitochondrion (OGG1) lyase activity (OGG1) hydrolase activity, acting on glycosyl bonds (OGG1) nucleolus (OGG1) nucleus (OGG1)

Anatomy Link Frequency HeLa 4 plasma 2 poly 1 Caco-2 1 HeLa S3 1 OGG1 (Homo sapiens)

Pain Link Frequency Relevance Heat Clonidine 51 99.12 Somatostatin 9 97.88 Glutamate 20 95.92 imagery 20 87.28 agonist 3 81.92 Chronic pancreatitis 1 76.08 Pain 20 74.56 ischemia 3 5.00 Inflammation 3 5.00 cINOD 1 5.00

Disease Link Frequency Relevance Heat Cancer 184 96.12 Contagious Ecthyma 20 87.52 Stress 42 79.92 Pancreatic Cancer 7 79.68 Pancreatitis 1 76.08 Pain 20 74.56 Apoptosis 12 38.64 Death 3 32.88 Carcinoma 20 32.48 Adenoma 20 32.08

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

A HeLa cell line stably expressing EGFP-hOGG1-Glu326 was generated and the subcellular localization was examined throughout the cell cycle (Figure 3).

Gene_expression (expressing) of EGFP-hOGG1 in HeLa

1) Confidence 0.74 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.05

However, no EGFP-hOGG1-Cys326 could be detected in the soluble chromatin or in the nuclear matrix fractions (Figure 7C, lanes 2 and 4).

Gene_expression (detected) of EGFP-hOGG1

2) Confidence 0.74 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.03

In order to elucidate the cellular role of hOGG1 we have investigated both the expression of hOGG1 and its cellular distribution.

Spec (investigated) Gene_expression (expression) of hOGG1

3) Confidence 0.64 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.13 Pain Relevance 0

To determine the distribution of EGFP-hOGG1-Cys326 during mitosis, we created stable cell lines overexpressing EGFP-hOGG1-Cys326.

Gene_expression (overexpressing) of EGFP-hOGG1

4) Confidence 0.64 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

In contrast, a recent study in which the promoter of HOGG1 was fused to the firefly luciferase gene carried out in HeLa cells showed that the expression of the reporter gene was not modulated during the cell cycle (71).

Gene_expression (expression) of HOGG1 in HeLa

5) Confidence 0.64 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.09 Pain Relevance 0

In contrast, a recent study in which the promoter of HOGG1 was fused to the firefly luciferase gene carried out in HeLa cells showed that the expression of the reporter gene was not modulated during the cell cycle (71).

Gene_expression (fused) of HOGG1 in HeLa

6) Confidence 0.64 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.09 Pain Relevance 0

EGFP-hOGG1 cells were synchronized at the G1/S boundary, released and allowed to enter S-phase before being treated with 0.04 ?

Gene_expression (synchronized) of EGFP-hOGG1

7) Confidence 0.64 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

The cell-cycle-dependent expression of HOGG1 was followed by northern blot hybridization of poly(A)+ RNA isolated from synchronized HaCat cells.

Gene_expression (expression) of HOGG1 in poly

8) Confidence 0.57 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.07 Pain Relevance 0.07

Cell-cycle-dependent expression of HOGG1 gene

Gene_expression (expression) of HOGG1

9) Confidence 0.57 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.07 Pain Relevance 0.07

Immunoblotting with anti-hOGG1 antibody revealed EGFP-hOGG1-Cys326 to be present only in the cytoplasmic fraction (Figure 7C, lane 1).

Gene_expression (present) of EGFP-hOGG1

10) Confidence 0.57 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

Transient transfection of HeLa cells with EGFP-hOGG1-Ala326 showed the distribution to be identical to that of EGFP-hOGG1-Cys326 indicating that the presence of the serine residue is important for the correct localization of hOGG1 to the nucleoli (data not shown).

Gene_expression (transfection) of EGFP-hOGG1 in HeLa

11) Confidence 0.56 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

However, endogenous hOGG1 is barely detected using the antibodies that are presently available [(23) and L.

Gene_expression (detected) of hOGG1

12) Confidence 0.56 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.07 Pain Relevance 0

We transiently co-transfected HeLa S3 cells with EYFP-hOGG1 and ECFP-PCNA and followed the cells for 20 h.

Gene_expression (transfected) of EYFP-hOGG1 in HeLa S3

13) Confidence 0.56 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

Overexpression of EGFP and EGFP-hOGG1 in the stable cell lines was examined in nuclear extracts by western blot using anti-OGG1 and anti-GFP specific antibodies.

Spec (examined) Gene_expression (Overexpression) of EGFP-hOGG1

14) Confidence 0.56 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

Site-directed mutagenesis of hOGG1

Gene_expression (mutagenesis) of hOGG1

15) Confidence 0.56 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

The associations with interphasic chromatin and nuclear matrix, and with mitotic condensed chromosomes are disrupted in EGFP-hOGG1-Cys326

Gene_expression (disrupted) of EGFP-hOGG1

16) Confidence 0.56 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

In this study, we investigated the subcellular localization and expression of hOGG1 during the cell cycle.

Spec (investigated) Gene_expression (expression) of hOGG1

17) Confidence 0.50 Published 2005 Journal Nucleic Acids Research Section Abstract Doc Link PMC1072800 Disease Relevance 0.31 Pain Relevance 0

Northern blot hybridization of synchronized HaCat cells showed a cell-cycle-dependent mRNA expression of hOGG1 with increased transcription of the major nuclear and mitochondrial isoforms during S/G2 (Figure 1).

Gene_expression (expression) of hOGG1

18) Confidence 0.50 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.03

By using a cell line constitutively expressing hOGG1 fused to enhanced green fluorescence protein (EGFP), we observed a dynamic relocalization of EGFP-hOGG1 to the nucleoli during the S-phase of the cell cycle, and this localization was shown to be linked to transcription.

Gene_expression (expressing) of hOGG1

19) Confidence 0.50 Published 2005 Journal Nucleic Acids Research Section Abstract Doc Link PMC1072800 Disease Relevance 0.30 Pain Relevance 0

To detect EGFP and EGFP-hOGG1 (and the mutants) the membrane was probed with a polyclonal anti-GFP antibody (ab290-50; Abcam), and to detect EGFP-hOGG1 (and the mutants) a monoclonal anti-human OGG1 antibody (clone 7E2; IBL) was used.

Gene_expression (detect) of EGFP-hOGG1

20) Confidence 0.50 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

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