INT269444

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Context Info
Confidence 0.04
First Reported 2008
Last Reported 2008
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 28
Disease Relevance 0
Pain Relevance 5.79

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

plasma membrane (RAB5A, HHIP) cell differentiation (RAB5A) extracellular region (HHIP)
carbohydrate metabolic process (HHIP) GTPase activity (RAB5A) cytoplasm (HHIP)
Anatomy Link Frequency
upper 5
vesicles 4
plasma 1
RAB5A (Homo sapiens)
HHIP (Homo sapiens)
Pain Link Frequency Relevance Heat
agonist 2968 99.34 Very High Very High Very High
imagery 196 96.14 Very High Very High Very High
dopamine receptor 28 25.32 Quite Low
Dopamine 28 16.88 Low Low
cINOD 28 5.00 Very Low Very Low Very Low
ischemia 28 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Increased Venous Pressure Under Development 28 47.60 Quite Low
Thrombosis 56 45.36 Quite Low
Cv Unclassified Under Development 84 43.68 Quite Low
Stroke 84 42.56 Quite Low
Myocardial Infarction 56 41.68 Quite Low
Disease 28 29.60 Quite Low
Coronary Artery Disease 56 5.00 Very Low Very Low Very Low
Cancer 28 5.00 Very Low Very Low Very Low
INFLAMMATION 28 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Moreover, through further co-immunoprecipitations, it was established that similar to the wild type Rab5a, both Rab5aS34N and Rab5aQ79L are capable of interacting with the hIP and that such interactions were enhanced on agonist-stimulation.
Rab5a Binding (interacting) of hIP associated with agonist
1) Confidence 0.04 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.26
Taken together, these data confirm that the hIP can physically and constitutively interact with Rab5a in the absence of agonist but that there is an increased association in response to agonist activation.
Rab5a Binding (interact) of hIP associated with agonist
2) Confidence 0.04 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.28
Moreover, through further co-immunoprecipitations, it was established that similar to the wild type Rab5a, both Rab5aS34N and Rab5aQ79L are capable of interacting with the hIP and that such interactions were enhanced on agonist-stimulation.
Rab5aQ79L Binding (interacting) of hIP associated with agonist
3) Confidence 0.04 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.26
Moreover, through further co-immunoprecipitations, it was established that similar to the wild type Rab5a, both Rab5aS34N and Rab5aQ79L are capable of interacting with the hIP and that such interactions were enhanced on agonist-stimulation.
Rab5aS34N Binding (interacting) of hIP associated with agonist
4) Confidence 0.04 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.26
Bearing this in mind, through a series of co-immunoprecipitations, it was next sought to investigate whether the hIP may physically/directly interact with Rab5, such as in response to agonist-stimulation.
Rab5 Binding (interact) of hIP associated with agonist
5) Confidence 0.04 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.20
On the other hand, in the co-immunoprecipitations, all HA-tagged hIPs (both cell surface and intracellular) were immunoprecipitated and hence, as is suggested from the co-immunoprecipitations, Rab5 associates with the hIP even in the absence of agonist.
Rab5 Binding (associates) of hIP associated with agonist
6) Confidence 0.04 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.26
Furthermore, following cicaprost stimulation, there was an increase in the amount of both GFP-Rab5a and endogenous Rab5 associated with the hIP immunoprecipitates (Fig. 5; upper panel).
Rab5 Binding (associated) of hIP in upper
7) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.22
Hence, these data indicate that the hIP located at the cell surface internalizes to intracellular Rab5-positive endosomes in response to cicaprost stimulation.
Rab5 Binding (internalizes) of hIP
8) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.15
Taken together, these data strongly suggest that Rab5a has an active role in the internalization of the hIP and is necessary for its efficient sorting to Rab5a-positive endosomes.
Rab5a Binding (internalization) of hIP
9) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.11
Moreover, while over-expression of a dominant negative form of Rab5a, namely Rab5aS34N, did not appear to affect the initial rate or overall biphasic profile of cicaprost-induced hIP internalization over the 4 h incubation period, it significantly impaired hIP internalization relative to that of the wild type Rab5a (Fig. 1F; p = 0.0003; ANOVA), with most significance at 2 and 3 h (Fig. 1E; p = 0.05 and p = 0.0001, respectively).
Rab5a Binding (internalization) of hIP
10) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.03
Confocal imaging of HEK.hIP cells over-expressing the GFP-tagged Rab5a demonstrated that there was rapid co-localization of the hIP to Rab5a positive vesicles at or just below the cell surface indicating that Rab5a affects internalization of the hIP at an early stage.
Rab5a Binding (internalization) of hIP in vesicles associated with imagery
11) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.17
Therefore, in view of the significant agonist-induced relocalization of the hIP away from the plasma membrane to the large intracellular vesicular or endosomal structures, as was evident from our immunolocalization data (Fig. 1C), we investigated the possible involvement of Rab5 in the internalization of the hIP.
Rab5 Binding (internalization) of hIP in plasma associated with agonist
12) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.17
The extent of association between the hIP and Rab5aS34N was similar to that of wild type Rab5a (Fig. 5; upper panel) as were the amount of hIP present in the immunoprecipitates and the levels of GFP-Rab5a and -Rab5aS34N over-expression in the HEK.hIP cells (Fig. 5, middle and lower panels, respectively).
Rab5aS34N Binding (association) of hIP in upper
13) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.25
Furthermore, we have demonstrated that there is a direct interaction between the hIP and Rab5a.
Rab5a Binding (interaction) of hIP
14) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.16
Our data established that the hIP is internalized in an agonist-dependent manner into Rab5a-containing endosomes through a mechanism that promoted its sorting into enlarged endocytic vesicles and involves a direct physical interaction between the hIP and Rab5a.


Rab5a Binding (interaction) of hIP in vesicles associated with agonist
15) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.13
Moreover, hIP:Rab5a interaction is somewhat independent of the guanine nucleotide (GDP/GTP) binding status suggesting that the Switch I and II domains of Rab5 do not greatly influence that interaction.


Rab5a Binding (interaction) of hIP
16) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.30
Similarly, immuno-detection with anti-GFP showed that the hIP and Rab5a interaction was enhanced in response to agonist (data not shown).
Rab5a Binding (interaction) of hIP associated with agonist
17) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.25
Co-immunoprecipitation studies confirmed a direct physical interaction between the hIP and Rab5a that was augmented by cicaprost.
Rab5a Binding (interaction) of hIP
18) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Abstract Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.15
Furthermore, agonist stimulation caused an increased association between the hIP and Rab5aS34N (Fig. 5; upper panel).
Rab5aS34N Binding (association) of hIP in upper associated with agonist
19) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.26
Similarly, immuno-detection with anti-GFP showed that the hIP and Rab5a interaction was enhanced in response to agonist (data not shown).
Rab5a Binding (interaction) of hIP associated with agonist
20) Confidence 0.03 Published 2008 Journal Biochim Biophys Acta Section Body Doc Link PMC2680976 Disease Relevance 0 Pain Relevance 0.25

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