INT269869

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Context Info
Confidence 0.77
First Reported 2009
Last Reported 2009
Negated 1
Speculated 2
Reported most in Body
Documents 1
Total Number 53
Disease Relevance 2.48
Pain Relevance 0

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleus (Sox17) DNA binding (Sox17) transcription factor binding (Sox17)
Anatomy Link Frequency
epithelial cells 15
lung 8
respiratory 6
proximal 4
duct 2
Sox17 (Mus musculus)
Pain Link Frequency Relevance Heat
ketamine 53 5.00 Very Low Very Low Very Low
anesthesia 53 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Repression 371 97.88 Very High Very High Very High
Hyperplasia 106 97.80 Very High Very High Very High
Targeted Disruption 212 97.30 Very High Very High Very High
Cancer 53 60.96 Quite High
Hypoglycemia 53 56.36 Quite High
Colon Cancer 53 46.84 Quite Low
Breast Cancer 53 37.80 Quite Low
Apoptosis 53 30.56 Quite Low
Injury 106 18.64 Low Low
Exsanguination 53 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To assess the molecular mechanisms by which Sox17 initiates cell cluster formation in respiratory epithelial progenitor cells, Sox17 was conditionally expressed in adult CCSPrtTA/tetO-Sox17 mice for 1, 3, or 5 days.
Gene_expression (expressed) of Sox17 in respiratory
1) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Conditional expression of Sox17 in epithelial cells of the adult mouse lung demonstrated that cell cluster formation and respecification of alveolar progenitor cells toward proximal airway lineages were rapidly reversible processes.
Gene_expression (expression) of Sox17 in epithelial cells
2) Confidence 0.77 Published 2009 Journal PLoS ONE Section Abstract Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Thus, cyclin D1 mRNA and protein levels were significantly increased in lungs from CCSPrtTA/tetO-Sox17 mice just prior to the onset of proliferation, consistent with the concept that cyclin D1 contributes to the cell cycle reentry of mature respiratory epithelial cells following expression of Sox17.
Gene_expression (expression) of Sox17 in epithelial cells
3) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Together, these results demonstrate that Sox17 expression in adult mouse lung results in decreased levels of cyclin-dependent kinase inhibitors associated with G1 arrest and increased expression of cell cycle-promoting genes, providing insight into the molecular mechanisms that regulate Sox17-induced proliferation associated with the initiation of progenitor cell behavior in respiratory epithelial cells.


Gene_expression (expression) of Sox17 in respiratory
4) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
To determine the effects of prolonged expression of Sox17 in respiratory epithelial cells, adult CCSPrtTA/tetO-Sox17 mice were maintained on Dox for 12 months.
Gene_expression (expression) of Sox17 in epithelial cells
5) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Lungs from CCSPrtTA/tetO-Sox17 and CCSPrtTA single transgenic control mice were harvested after 4 weeks exposure to doxycycline (Dox) or following removal of Dox for 1 week, and were examined for expression of Sox17 and the proximal airway epithelial cell markers CCSP (Clara cells) and Foxj1 (ciliated cells).
Spec (examined) Gene_expression (expression) of Sox17 in Lungs associated with targeted disruption
6) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0.10 Pain Relevance 0
Together these data show that prolonged expression of Sox17 in the adult mouse lung dramatically influences respiratory epithelial cell differentiation, generating ectopic structures in the peripheral lung with characteristics of the more proximal bronchiolar epithelium.


Gene_expression (expression) of Sox17 in epithelium
7) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
In lungs from CCSPrtTA/tetO-Sox17 mice in which Dox treatment was discontinued for 1 week prior to harvest, immunostaining for Sox17 was only detected in endothelial cells in the peripheral lung and was indistinguishable from controls (Fig. 1A,C).
Gene_expression (detected) of Sox17 in endothelial cells
8) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0.08 Pain Relevance 0
Bronchiolar-like lesions are induced in the alveoli following prolonged Sox17 expression
Gene_expression (expression) of Sox17
9) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
To determine if alveolar cells become permanently respecified following ectopic Sox17 expression, Sox17 was conditionally expressed in respiratory epithelial cells of adult mice.
Gene_expression (expressed) of Sox17 in respiratory
10) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0.09 Pain Relevance 0
Constitutive expression of Sox17 in human embryonic stem cells is sufficient to commit cells to the definitive endoderm lineage, and increased expression of Sox17 is associated with the differentiation of embryonic stem cells toward cells with characteristics of the respiratory epithelium [5], [6], [10]–[12].
Gene_expression (expression) of Sox17 in epithelium
11) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Endogenous expression of Sox17 was detected in endothelial cells in the peripheral lung but not in airway epithelial cells.
Gene_expression (expression) of Sox17 in lung
12) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
In addition to increased expression of Sox17 (data not shown), mRNAs for the cyclin-dependent kinase inhibitors p15, p21, and p57 were significantly decreased in lungs of CCSPrtTA/tetO-Sox17 mice relative to CCSPrtTA controls following 1 day of exposure to Dox (Fig. 5).
Gene_expression (expression) of Sox17 in lungs
13) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Since Sox17 is highly expressed in the endoderm, which gives rise to the respiratory epithelium, Sox17 likely plays an early role in specification of endodermal precursor cells prior to emergence of the lung primordium.
Gene_expression (expressed) of Sox17 in epithelium
14) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Constitutive expression of Sox17 in human embryonic stem cells is sufficient to commit cells to the definitive endoderm lineage, and increased expression of Sox17 is associated with the differentiation of embryonic stem cells toward cells with characteristics of the respiratory epithelium [5], [6], [10]–[12].
Gene_expression (expression) of Sox17 in epithelium
15) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Long-term expression of Sox17 caused the formation of organized sheets of epithelial cells in the peripheral lung with morphological similarities to the bronchiolar epithelium (Fig. 2).
Gene_expression (expression) of Sox17 in epithelial cells
16) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Expression of Sox17 in peripheral respiratory epithelial cells induced the formation of atypical alveolar cell clusters which contained cells that expressed conducting airway epithelial cell markers CCSP and Foxj1 (Fig. 1B,E,H).
Gene_expression (Expression) of Sox17 in alveolar cell
17) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0.09 Pain Relevance 0
Further, while expression of p19 was variably decreased in lungs from CCSPrtTA/tetO-Sox17 mice maintained on Dox for 2 days, no differences in the expression of p16 or p27 were observed after expression of Sox17 for 1–3 days (data not shown).
Gene_expression (expression) of Sox17 in lungs
18) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Expression of Smad3 and Sox17 inputs was assessed by immunoblot using cell lysates from transfections done in parallel to chromatin immunoprecipitations.


Gene_expression (Expression) of Sox17
19) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
Although Sox17 is highly expressed in the endoderm and is required for its formation prior to the emergence of the lung primordium, it is not readily detected in the respiratory epithelium thereafter.
Gene_expression (expressed) of Sox17 in endoderm
20) Confidence 0.77 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0.07 Pain Relevance 0

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