INT270949

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.28
First Reported 2009
Last Reported 2009
Negated 0
Speculated 3
Reported most in Body
Documents 1
Total Number 26
Disease Relevance 0.25
Pain Relevance 3.19

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

peptidase activity (Cpe) extracellular space (Cpe) aging (Slc6a3)
Golgi apparatus (Cpe) plasma membrane (Slc6a3) transmembrane transport (Slc6a3)
Anatomy Link Frequency
striatum 4
Slc6a3 (Rattus norvegicus)
Cpe (Rattus norvegicus)
Pain Link Frequency Relevance Heat
Kinase C 390 100.00 Very High Very High Very High
Dopamine 806 97.24 Very High Very High Very High
monoamine 26 87.48 High High
Substantia nigra 26 81.76 Quite High
gABA 52 73.20 Quite High
anesthesia 26 52.96 Quite High
imagery 26 13.16 Low Low
Neurotransmitter 52 5.00 Very Low Very Low Very Low
Neuropeptide 52 5.00 Very Low Very Low Very Low
addiction 52 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Stress 78 69.68 Quite High
Obesity 52 65.60 Quite High
Disease 78 56.56 Quite High
Drug Dependence 52 5.00 Very Low Very Low Very Low
Sprains And Strains 26 5.00 Very Low Very Low Very Low
Neurological Disease 26 5.00 Very Low Very Low Very Low
Death 26 5.00 Very Low Very Low Very Low
Schizophrenia 26 5.00 Very Low Very Low Very Low
Cognitive Disorder 26 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Taken together, these results showed that DAT could associate with CPE via the DAT-CT.


DAT Binding (associate) of CPE
1) Confidence 0.28 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.11
Reduced phosphorylation state of DAT might reflect that association of CPE with DAT leaded to the occlusion of PKC phosphorylation sites.
DAT Spec (might) Binding (association) of CPE associated with kinase c
2) Confidence 0.28 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.20
The association of DAT with CPE may have critical impact on DAT phosphorylation state and be involved in cyclic trafficking.
DAT Binding (association) of CPE
3) Confidence 0.28 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0.12 Pain Relevance 0.18
In the present study, we provided biochemical evidence for a physical interaction between CPE and DAT via the DAT-CT.
DAT Binding (interaction) of CPE
4) Confidence 0.28 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.18
Taken together, these data indicated that the binding of DAT-CT tail583–620 sequences to CPE was both sufficient and necessary for the expression of CPE- mediated functional regulation of the DA translocation process.


DAT Binding (binding) of CPE associated with dopamine
5) Confidence 0.24 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.25
Taken together, these results showed that DAT could associate with CPE via the DAT-CT.


DAT Binding (associate) of CPE
6) Confidence 0.22 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.11
Reduced phosphorylation state of DAT might reflect that association of CPE with DAT leaded to the occlusion of PKC phosphorylation sites.
DAT Spec (might) Binding (association) of CPE associated with kinase c
7) Confidence 0.22 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.20
As shown in Figure 1C, in vitro translated [35S]-CPE probe hybridized with GST-DAT-CT but not GST-DAT-NT or GST alone, indicating the specificity of the direct protein-protein interaction between CPE and DAT-CT.
DAT Binding (interaction) of CPE
8) Confidence 0.21 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0
As shown in Fig 1B, GST-DAT-NT was unable to precipitate CPE, strongly suggesting that the interaction between DAT and CPE is mediated specifically by the DAT-CT.
DAT Binding (interaction) of CPE
9) Confidence 0.21 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.03
Here, we have identified that carboxypeptidase E (CPE), a prohormone processing exopeptidase and sorting receptor for the regulated secretory pathway, interacts with the DAT-CT and affects DAT function.
DAT Binding (interacts) of carboxypeptidase E
10) Confidence 0.21 Published 2009 Journal Mol Brain Section Abstract Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.17
As shown in Fig 4B, PMA treatment reduced the association between CPE and DAT as compared with non-treated COS-7 cells, implying that CPE preferentially interacted with non-PKC phosphorylated forms of DAT.


DAT Binding (interacted) of CPE associated with kinase c
11) Confidence 0.21 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.26
As shown in Fig 4B, PMA treatment reduced the association between CPE and DAT as compared with non-treated COS-7 cells, implying that CPE preferentially interacted with non-PKC phosphorylated forms of DAT.


DAT Binding (association) of CPE associated with kinase c
12) Confidence 0.21 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.23
In vitro binding assays were performed to provide evidence that DAT and CPE can directly interact with each other.
DAT Binding (interact) of CPE
13) Confidence 0.21 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0
In the present study, we provided biochemical evidence for a physical interaction between CPE and DAT via the DAT-CT.
DAT Binding (interaction) of CPE
14) Confidence 0.21 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.18
To further confirm the interaction between DAT and CPE, we performed GST affinity pull down assay using purified GST fusion peptides containing sequences from DAT-CT (GST-DAT-CT).
DAT Binding (interaction) of CPE
15) Confidence 0.21 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0.07 Pain Relevance 0.09
Here, we have identified that carboxypeptidase E (CPE), a prohormone processing exopeptidase and sorting receptor for the regulated secretory pathway, interacts with the DAT-CT and affects DAT function.
DAT Binding (interacts) of CPE
16) Confidence 0.21 Published 2009 Journal Mol Brain Section Abstract Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.17
It is possible that the interaction with CPE may mask the PKC phosphorylation site on DAT.
DAT Binding (interaction) of CPE associated with kinase c
17) Confidence 0.19 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.32
Next, we examined in solubilized rat striatum, whether DAT could coimmunopreicipiate with CPE thus providing additional evidence of a physical association between DAT and CPE.
DAT Binding (coimmunopreicipiate) of CPE in striatum
18) Confidence 0.19 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.06
As shown in Figure 1C, in vitro translated [35S]-CPE probe hybridized with GST-DAT-CT but not GST-DAT-NT or GST alone, indicating the specificity of the direct protein-protein interaction between CPE and DAT-CT.
GST-DAT-NT Binding (hybridized) of CPE
19) Confidence 0.19 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0
Taken together, these data suggest that CPE is involved in a direct protein-protein interaction with DAT-CT.
DAT Binding (interaction) of CPE
20) Confidence 0.18 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.03

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox