INT272206

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Context Info
Confidence 0.77
First Reported 2007
Last Reported 2007
Negated 1
Speculated 1
Reported most in Body
Documents 4
Total Number 26
Disease Relevance 3.83
Pain Relevance 0.70

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

intracellular (CAPN3) protein complex assembly (CAPN3) protein complex (CAPN3)
cytoplasm (CAPN3) signal transducer activity (CAPN3) cytosol (CAPN3)
Anatomy Link Frequency
skeletal muscles 3
skeletal muscle cells 2
spike 1
Limb 1
CAPN3 (Homo sapiens)
Pain Link Frequency Relevance Heat
ischemia 46 99.42 Very High Very High Very High
Pain 49 99.04 Very High Very High Very High
imagery 23 94.40 High High
addiction 23 19.84 Low Low
tolerance 23 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Autolysis 118 100.00 Very High Very High Very High
Targeted Disruption 23 99.88 Very High Very High Very High
Cv Unclassified Under Development 23 99.42 Very High Very High Very High
Pain 49 99.04 Very High Very High Very High
Muscular Dystrophy 97 97.94 Very High Very High Very High
Disease 52 89.08 High High
Muscular Atrophy 9 86.52 High High
Apoptosis 23 85.12 High High
Muscle Weakness 3 76.96 Quite High
Death 23 73.36 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Although the expected advantage of our COS7 system is that ectopically expressed p94WT is the only active cal-pain present when cells were not otherwise stimulated, the lack of stimuli to spike signaling cascades such as [Ca2+]i regulation or cytoskeletal motility might hamper contact between p94 and substrates and/or other molecules and enhance autolytic rather than proteolytic reaction.
Gene_expression (expressed) of p94WT in spike associated with pain
1) Confidence 0.77 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.25 Pain Relevance 0.15
In COS7 cells, overexpressed p94 disappears very rapidly because of its autolytic activity (see below) [26] and simultaneously causes specific proteolysis of other proteins such as calpastatin and fodrin [17].
Gene_expression (overexpressed) of p94
2) Confidence 0.77 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0 Pain Relevance 0.05
An alternative ubiquitous promoter of CAPN3 was recently identified and the expression of several p94 variants in various organs, including the brain, has been reported [22–24], suggesting that p94 is important for tissues other than skeletal muscles.
Gene_expression (expression) of p94 in skeletal muscles
3) Confidence 0.77 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.26 Pain Relevance 0
To identify proteins targeted by p94 protease activity, quantitative proteomic analysis of COS7 cells expressing either p94 WT or CS was performed using the iTRAQ™ technique and 2-D LC-MALDI-MS/MS analysis.
Gene_expression (expressing) of p94
4) Confidence 0.77 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0 Pain Relevance 0.04
Using COS7 cells transfected with a p94 expression vector and Western blot analyses, we previously detected proteolysis of several proteins, suggesting that p94 prote-olytic activity is readily exerted and that the proteins identified are potential in vivo p94 substrates [17].
Spec (analyses) Gene_expression (expression) of p94
5) Confidence 0.77 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.22 Pain Relevance 0
Quantitative comparison of proteomic data obtained from COS7 cells expressing p94WT or p94CS was efficient in surveying the change of protein abundance caused by p94 protease activity.
Gene_expression (expressing) of p94WT
6) Confidence 0.77 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0 Pain Relevance 0
It is particularly important to distinguish direct p94 substrates from those proteolyzed by other proteases activated by overexpression of p94.
Gene_expression (overexpression) of p94
7) Confidence 0.77 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.07 Pain Relevance 0
The identification of filamin-A and vimentin as substrates is consistent with the observation that ectopic expression of p94 in several different culture cells causes morphological defects accompanied by proteolysis of several cytoskeletal proteins, including filamin-A and fodrin [40].
Gene_expression (expression) of p94
8) Confidence 0.77 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.07 Pain Relevance 0
Clinical features of the patients with calpain 3 deficiency
Gene_expression (deficiency) of calpain 3
9) Confidence 0.67 Published 2007 Journal Journal of Korean Medical Science Section Body Doc Link PMC2693639 Disease Relevance 0.14 Pain Relevance 0.08
Clinical analysis of patients with calpain 3 deficiency
Gene_expression (deficiency) of calpain 3
10) Confidence 0.67 Published 2007 Journal Journal of Korean Medical Science Section Body Doc Link PMC2693639 Disease Relevance 0.20 Pain Relevance 0
Although he showed loss of immunoreactivity against all the three sets of anti-calpain 3 antibodies, we were not able to find any CAPN3 mutations.
Gene_expression (antibodies) of calpain 3
11) Confidence 0.67 Published 2007 Journal Journal of Korean Medical Science Section Body Doc Link PMC2693639 Disease Relevance 0.24 Pain Relevance 0
Proteins represented by peptides with reliable MS/MS spectra and whose signal ratios were decreased by WT p94 expression are listed in Table 2.
Gene_expression (expression) of p94
12) Confidence 0.67 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0 Pain Relevance 0
This was probably caused in part by much less efficient protein proteolysis by p94 than p94 autolysis, which could be because of that COS7 cells but not skeletal muscle cells were used.
Gene_expression (autolysis) of p94 in skeletal muscle cells associated with autolysis
13) Confidence 0.66 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.25 Pain Relevance 0.15
As our previous study [28] demonstrated that p94 has substrate specificities very similar to those of the ?
Gene_expression (has) of p94
14) Confidence 0.66 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.26 Pain Relevance 0
This was probably caused in part by much less efficient protein proteolysis by p94 than p94 autolysis, which could be because of that COS7 cells but not skeletal muscle cells were used.
Gene_expression (autolysis) of p94 in skeletal muscle cells associated with autolysis
15) Confidence 0.66 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.25 Pain Relevance 0.15
The primary structure of p94 is very similar to that of the catalytic subunits of the ?
Gene_expression (structure) of p94
16) Confidence 0.66 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.30 Pain Relevance 0
Among the proteins listed in Table 2, p94 was ranked as the protein with the greatest decrease (averaged Rmin for peptides = 3.024, n = 5) (Fig. 4G).
Gene_expression (ranked) of p94
17) Confidence 0.66 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0 Pain Relevance 0
All five (four unique) peptides (Fig. 5C "p94") detected for p94 decreased in WT samples to a similar extent.
Gene_expression (detected) of p94
18) Confidence 0.66 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.06 Pain Relevance 0
By Western blot analysis, fodrin, one of the few in vivo substrates of conventional calpains that is proteolyzed during ischemia, was readily detected to be proteolyzed by p94.
Gene_expression (proteolyzed) of p94 associated with ischemia
19) Confidence 0.66 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.24 Pain Relevance 0.05
An alternative ubiquitous promoter of CAPN3 was recently identified and the expression of several p94 variants in various organs, including the brain, has been reported [22–24], suggesting that p94 is important for tissues other than skeletal muscles.
Gene_expression (expression) of CAPN3 in skeletal muscles
20) Confidence 0.66 Published 2007 Journal Biotechnology Journal Section Body Doc Link PMC2978325 Disease Relevance 0.26 Pain Relevance 0

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