INT274609

Context Info Confidence 0.45 First Reported 2009 Last Reported 2009 Negated 1 Speculated 0 Reported most in Body Documents 1 Total Number 3 Disease Relevance 0.44 Pain Relevance 0

cytosol (PSTPIP1) signal transduction (PSTPIP1) oxidoreductase activity (PSTPIP1) cell adhesion (PSTPIP1) cytoskeleton (PSTPIP1) cytoplasm (PSTPIP1)

Anatomy Link Frequency filaments 2 PSTPIP1 (Homo sapiens)

Pain Link Frequency Relevance Heat Inflammation 27 5.00 Pain 6 5.00 Arthritis 6 5.00

Disease Link Frequency Relevance Heat Syndrome 27 86.36 Disease 30 77.76 Fever 33 73.96 INFLAMMATION 27 5.00 Osteomyelitis 12 5.00 Pain 6 5.00 Arthritis 6 5.00 Acne 6 5.00 Pyoderma Gangrenosum 6 5.00 Adhesions 3 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

To explore the effect of PAPA mutations on the PSTPIP1:pyrin interaction, we next tested whether the filaments formed by mutant forms of PSTPIP1 could be reorganized by pyrin.

Regulation (effect) of PSTPIP1 Binding (interaction) of in filaments

1) Confidence 0.45 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2702820 Disease Relevance 0.15 Pain Relevance 0

Similarly, in previous studies using immunoprecipitation, mutations in pyrin did not modify its interaction with PSTPIP1 [5].

Neg (not) Regulation (modify) of PSTPIP1 Neg (not) Binding (interaction) of

2) Confidence 0.45 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2702820 Disease Relevance 0.15 Pain Relevance 0

This corroborates earlier reports that mutant forms of PSTPIP1 bind to pyrin with higher affinity [5], and confirms that PSTPIP1 recruitment to the speck compartment depends on the PSTPIP1-pyrin interaction.

Regulation (depends) of PSTPIP1 Binding (recruitment) of

3) Confidence 0.27 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2702820 Disease Relevance 0.14 Pain Relevance 0

General Comments

This test has worked.