INT27608

Context Info Confidence 0.61 First Reported 1989 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 7 Total Number 7 Disease Relevance 2.66 Pain Relevance 1.69

cytosol (Alox5) nuclear envelope (Alox5) oxidoreductase activity (Alox5) nucleus (Alox5) cytoplasm (Alox5)

Anatomy Link Frequency macrophage 2 Alox5 (Mus musculus)

Pain Link Frequency Relevance Heat cINOD 38 99.40 Inflammation 130 99.04 IPN 2 97.76 Hyperalgesia 1 96.24 antagonist 7 95.28 aspirin 13 85.84 Analgesic 5 84.88 Paracetamol 2 80.88 diclofenac 4 77.36 Pain 3 63.84

Disease Link Frequency Relevance Heat INFLAMMATION 141 99.04 Pancreatic Cancer 84 98.68 Cancer 85 98.36 Inflammatory Pain 2 97.76 Pneumonia 3 97.70 Hyperalgesia 1 96.24 Hypotension 1 88.40 Lung Cancer 9 83.44 Leukemia 2 81.16 Colon Cancer 6 79.04

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Table 1 shows primer sequences used to detect 5-LOX, DNMTs, and cyclophilin mRNAs (primers were purchased from Integrated DNA Technologies, Inc., Coralville, IA).

Localization (detect) of 5-LOX

1) Confidence 0.61 Published 2009 Journal Neural Plasticity Section Body Doc Link PMC2801004 Disease Relevance 0.08 Pain Relevance 0.08

We found that the number of motifs which describe the colocalization of 5-lipoxygenase (5-LO) or 12-LO with other proteins increased disproportionally after dipyrone treatment.

Localization (colocalization) of 5-LO

2) Confidence 0.54 Published 2009 Journal J. Proteome Res. Section Abstract Doc Link 19697962 Disease Relevance 0.27 Pain Relevance 0.28

Chronic administration of butylated hydroxytoluene (BHT) to mice stimulates pulmonary inflammation characterized by vascular leakage and macrophage infiltration into the air spaces, increased PGE2 production, and translocation of 5-lipoxygenase (5-LO) from the cytosol to the particulate fraction.

Localization (translocation) of 5-LO in macrophage associated with inflammation and pneumonia

3) Confidence 0.53 Published 2002 Journal Carcinogenesis Section Abstract Doc Link 12376474 Disease Relevance 0.88 Pain Relevance 0.39

The macrophage plays a major role in the immune response and the inflammatory process, it has the capacity to synthesize and secrete arachidonic acid oxygenation products derived from both cyclooxygenase and lipoxygenase catalyzed pathways, and has been used extensively to study the effect of inhibitors of phospholipases, cyclooxygenase and lipoxygenase enzymes.

Localization (secrete) of lipoxygenase in macrophage associated with inflammation

4) Confidence 0.10 Published 1989 Journal Medicina (B Aires) Section Abstract Doc Link 2640487 Disease Relevance 0.25 Pain Relevance 0.26

Feasibility of targeting cyclooxygenase and lipoxygenase for treatment and prevention of pancreatic cancer

Localization (cyclooxygenase) of lipoxygenase associated with pancreatic cancer

5) Confidence 0.07 Published 2003 Journal Mol Cancer Section Body Doc Link PMC149414 Disease Relevance 0.67 Pain Relevance 0.06

AA is metabolized by three major oxidative pathways: 1) cyclooxygenase (COX), forming prostaglandins and related eicosanoids; 2) lipoxygenase (LOX), forming leukotrienes and related compounds; and 3) CYP450, forming epoxides (2C/2J) and 20-HETEs (4A/4F).

Localization (metabolized) of lipoxygenase

6) Confidence 0.05 Published 2010 Journal Curr Atheroscler Rep Section Body Doc Link PMC2857794 Disease Relevance 0.35 Pain Relevance 0.17

These authors concluded that the mechanism of action of these NSAIDs is not related to their COX, lipoxygenase, NF?

Localization (lipoxygenase) of lipoxygenase associated with cinod

7) Confidence 0.01 Published 2007 Journal Current Neuropharmacology Section Body Doc Link PMC2644496 Disease Relevance 0.16 Pain Relevance 0.44

General Comments

This test has worked.