INT281277

Context Info Confidence 0.41 First Reported 2009 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 6 Disease Relevance 0.93 Pain Relevance 0.27

cell differentiation (Stx2)

Anatomy Link Frequency monocyte 2 blood 1 neutrophils 1 Stx2 (Mus musculus)

Pain Link Frequency Relevance Heat agonist 54 97.60 Inflammation 18 5.00 cytokine 12 5.00 withdrawal 6 5.00 Crohn's disease 6 5.00 Angina 6 5.00

Disease Link Frequency Relevance Heat Stress 42 91.84 Hemolytic Uremic Syndrome 246 86.16 Heart Rate Under Development 30 76.08 Thrombotic Microangiopathies 18 56.16 Sepsis 18 54.52 Cv Unclassified Under Development 6 50.60 Endotoxemia 24 47.16 Acute Coronary Syndrome 6 44.88 Thrombosis 6 39.52 Disease 36 33.20

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Stx2 and EHEC-LPS induce tissue factor–positive microparticles

Positive_regulation (induce) of Stx2

1) Confidence 0.41 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0.06

Co-stimulation with Stx2 and LPS resulted in an additive effect with regard to platelet-monocyte aggregate formation and TF expression on these aggregates, particularly when high shear forces were applied.

Positive_regulation (stimulation) of Stx2 in monocyte

2) Confidence 0.41 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.52 Pain Relevance 0

Taken together these results show that Stx2, O157LPS and Stx2/LPS induce a significant increase in TF expression, predominantly on platelet-monocyte aggregates, that the combination of Stx2 and LPS had an additive effect on TF-expression on platelet-monocyte aggregates, and that O157LPS was slightly more potent than the other LPS serotypes.

Positive_regulation (combination) of Stx2 in monocyte

3) Confidence 0.41 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0

Stx2 and EHEC-LPS induce tissue factor–positive microparticles

Positive_regulation (microparticles) of Stx2

4) Confidence 0.41 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0.06

Stx2, LPS (O157:H7 or O111:B4) or Stx2/LPS were added to whole blood immediately before perfusion through a parallel plate flow chamber.

Positive_regulation (/) of Stx2 in blood

5) Confidence 0.41 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.20 Pain Relevance 0.15

Stx2 stimulation increased CD11b expression in platelet-bound as well as in platelet-free monocytes whereas only a weak increase was detected in neutrophils in complex with platelets and no increase was seen in platelet-free neutrophils (Figure 3C).

Positive_regulation (stimulation) of Stx2 in neutrophils

6) Confidence 0.38 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.21 Pain Relevance 0

General Comments

This test has worked.