INT281285

Context Info Confidence 0.63 First Reported 2009 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 12 Disease Relevance 5.14 Pain Relevance 0.38

cell differentiation (Stx2)

Anatomy Link Frequency blood 3 monocyte 3 Plasma 1 leukocyte 1 11E10 1 Stx2 (Mus musculus)

Pain Link Frequency Relevance Heat agonist 108 99.12 withdrawal 12 94.08 Crohn's disease 12 7.68 Inflammation 36 5.00 cytokine 24 5.00 Angina 12 5.00

Disease Link Frequency Relevance Heat Hemolytic Uremic Syndrome 492 99.96 Disease 72 99.88 Stress 84 96.16 Infection 24 95.84 Acute Renal Failure 60 90.96 Thrombocytopenia 36 88.84 Hemolytic Anemia 24 85.72 Urinary Tract Infection 12 76.72 Neuroblastoma 12 74.48 Hypersensitivity 12 72.04

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Stx2 is present on platelet-monocyte and platelet-neutrophil aggregates from patients with HUS

Gene_expression (present) of Stx2 in monocyte associated with hemolytic uremic syndrome

1) Confidence 0.63 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.67 Pain Relevance 0

Fecal isolates were positive for Stx2-producing E. coli O157:H7 in three cases and in the fourth case for Stx1- and Stx2- producing E. coli O98:H21.

Gene_expression (producing) of Stx2

2) Confidence 0.63 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 1.06 Pain Relevance 0

A significant increase in platelet microparticle generation and numbers of microparticles expressing TF was noted in whole blood co-stimulated with Stx2/LPS compared to each agonist alone (P<0.05).

Gene_expression (expressing) of Stx2 in blood associated with agonist

3) Confidence 0.54 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0.14

500, PBS:blood), Stx2, O157:H7LPS, O111:B4LPS or a combination of Stx2/O157:H7LPS or Stx2/O111:B4LPS were added to the blood.

Gene_expression (/) of Stx2 in blood

4) Confidence 0.54 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.07 Pain Relevance 0.05

Erythrocytes were lysed with FACSLyse and cells were washed twice with PBS and incubated with a mouse monoclonal anti-Stx2 IgG1 antibody (11E10, 200 ng/mL, a gift from T.G.

Gene_expression (antibody) of Stx2 in 11E10

5) Confidence 0.54 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0.03

Incubation of whole blood with a combination of Stx2 and LPS (O157:H7LPS or O111:B4LPS) induced a significant increase in platelet-monocyte aggregates as well as platelet-free monocytes expressing TF compared to Stx2 or LPS alone.

Gene_expression (expressing) of Stx2 in monocyte

6) Confidence 0.54 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0

Both Stx2 and LPS induced platelet-leukocyte aggregate formation, TF expression and microparticle generation.

Gene_expression (expression) of Stx2 in leukocyte

7) Confidence 0.54 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.65 Pain Relevance 0

500, PBS:blood), Stx2, O157:H7LPS, O111:B4LPS or a combination of Stx2/O157:H7LPS or Stx2/O111:B4LPS were added to the blood.

Gene_expression (/) of Stx2 in blood

8) Confidence 0.54 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.07 Pain Relevance 0.05

Stx2 was detected on circulating platelet-monocyte (median 39%, range 22–49%) and platelet-neutrophil aggregates (median 24%, range 9–43%) from all patients with HUS during the acute phase of disease.

Gene_expression (detected) of Stx2 in monocyte associated with hemolytic uremic syndrome and disease

9) Confidence 0.49 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.73 Pain Relevance 0

Fecal isolates were positive for Stx2-producing E. coli O157:H7 in three cases and in the fourth case for Stx1- and Stx2- producing E. coli O98:H21.

Gene_expression (producing) of Stx2

10) Confidence 0.49 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 1.05 Pain Relevance 0

Plasma levels of functional TF were examined after incubation of whole blood with Stx2, O157:H7LPS or O111:B4LPS or LPS/Stx2 for 4 h.

Gene_expression (/) of Stx2 in Plasma

11) Confidence 0.47 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.17 Pain Relevance 0.12

Stx2 is present on platelet-monocyte and platelet-neutrophil aggregates from patients with HUS

Gene_expression (present) of Stx2 in platelet associated with hemolytic uremic syndrome

12) Confidence 0.21 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.67 Pain Relevance 0

General Comments

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