INT281286

Context Info Confidence 0.39 First Reported 2009 Last Reported 2009 Negated 2 Speculated 1 Reported most in Body Documents 1 Total Number 13 Disease Relevance 2.02 Pain Relevance 0.55

cell differentiation (Stx2)

Anatomy Link Frequency platelet 7 neutrophils 3 monocytes 1 leukocytes 1 blood cells 1 Stx2 (Mus musculus)

Pain Link Frequency Relevance Heat agonist 117 98.84 withdrawal 13 32.64 Inflammation 39 5.00 cytokine 26 5.00 Crohn's disease 13 5.00 Angina 13 5.00

Disease Link Frequency Relevance Heat Disease 78 98.72 Hemolytic Uremic Syndrome 533 97.36 Acute Renal Failure 65 97.32 Stress 91 93.00 Hypersensitivity 13 76.16 Heart Rate Under Development 65 48.80 Endotoxemia 52 5.00 Thrombocytopenia 39 5.00 Thrombotic Microangiopathies 39 5.00 Sepsis 39 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Stx2 binds to monocytes and to activated platelets

Stx2 Binding (binds) of in platelets

1) Confidence 0.39 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0.14

Results shown above indicated that Stx2 alone did not have as profound effect on platelet-leukocyte aggregate formation, platelet or leukocyte activation and TF-positive microparticle generation as LPS, and since this response was mostly demonstrated in monocytes, we examined the ability of Stx2 to bind to platelets, monocytes and neutrophils.

Stx2 Spec (examined) Binding (bind) of in platelet

2) Confidence 0.39 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.05 Pain Relevance 0.12

Stx2 binding was also examined on purified platelets, monocytes and neutrophils and binding was detected in a similar manner.

Stx2 Binding (binding) of in monocytes

3) Confidence 0.39 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0

There was no binding of Stx2 to platelet-bound neutrophils and no binding was detected when the primary anti-Stx2 antibody was omitted.

Stx2 Neg (no) Binding (binding) of in neutrophils

4) Confidence 0.39 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.08 Pain Relevance 0.10

Binding of Stx2 to platelet-leukocyte aggregates or to free (unbound) platelets, monocytes or neutrophils was assayed in whole blood, incubated with Stx2 (200 pg/mL) for 30 min at 37°C and the antibody combinations described above.

Stx2 Binding (Binding) of in neutrophils

5) Confidence 0.39 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0.03

Binding of Stx2 and O157LPS to platelets and leukocytes

Stx2 Binding (Binding) of in leukocytes

6) Confidence 0.39 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0.03

In addition, all patients had Stx2 bound to their platelet-free monocytes (median 37%, range 30–41%), platelet-free neutrophils (median 22, range 17–32%) as well as to their unbound-platelets (median 55%, range 39–82%).

Stx2 Binding (bound) of in platelet

7) Confidence 0.39 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.78 Pain Relevance 0

In the platelet-bound leukocyte population (gate two) the FL1 versus FL2 cytograms were used to estimate the percentage of platelet-leukocyte aggregates with bound TF or Stx2 (Figure 1C).

Stx2 Binding (bound) of in platelet

8) Confidence 0.34 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.08 Pain Relevance 0

As a control, no binding of the anti-stx2 antibody was detected on blood cells from the three patients with acute renal failure indicating that antibody binding was not due to cell changes related to acute renal failure.

stx2 Neg (no) Binding (binding) of in blood cells associated with acute renal failure

9) Confidence 0.34 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.85 Pain Relevance 0

Thus a most potent additive effect regarding TF-expression was noted on platelet-monocyte aggregates exposed to Stx2/LPS at high shear rates.

Stx2 Binding (exposed) of in platelet

10) Confidence 0.30 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0.18 Pain Relevance 0.10

Stx2 binding was also examined on purified platelets, monocytes and neutrophils and binding was detected in a similar manner.

Stx2 Binding (binding) of in platelets

11) Confidence 0.13 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0

Binding of Stx2 and O157LPS to platelets and leukocytes

Stx2 Binding (Binding) of in platelets

12) Confidence 0.13 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0.03

Stx2 binding was also examined on purified platelets, monocytes and neutrophils and binding was detected in a similar manner.

Stx2 Binding (binding) of in neutrophils

13) Confidence 0.13 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2735777 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.