INT28215

Context Info Confidence 0.76 First Reported 1989 Last Reported 2011 Negated 0 Speculated 12 Reported most in Body Documents 196 Total Number 208 Disease Relevance 113.90 Pain Relevance 18.30

cytosol (CDKN1A) nucleoplasm (CDKN1A) nucleus (CDKN1A) cell cycle (CDKN1A) kinase activity (CDKN1A) cytoplasm (CDKN1A)

Anatomy Link Frequency epithelial cell 7 MDA-MB-231 6 AGS 6 BxPC-3 5 cleavage 3 CDKN1A (Homo sapiens)

Pain Link Frequency Relevance Heat Calcitonin gene-related peptide 196 99.80 Paracetamol 34 99.78 agonist 164 99.74 dexamethasone 101 99.62 aspirin 42 99.56 narcan 189 99.52 withdrawal 37 99.28 fibrosis 57 98.54 Potency 24 98.38 cocaine 588 98.36

Disease Link Frequency Relevance Heat Apoptosis 3446 100.00 Retinoblastoma 625 100.00 Aging 92 100.00 Hyperplasia 62 100.00 Cancer 2992 99.98 Pancreatic Cancer 1011 99.84 Polycystic Kidney Disease 720 99.84 Cystic Kidney Diseases 120 99.84 Prostate Cancer 81 99.84 Non-small-cell Lung Cancer 77 99.84

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

We have found that p53 protein accumulated in a dose- and time-dependent manner after Dx treatment, while p21 expression increased over time with low but decreased with high Dx doses.

Gene_expression (expression) of p21

1) Confidence 0.76 Published 2002 Journal Carcinogenesis Section Abstract Doc Link 12151346 Disease Relevance 1.40 Pain Relevance 0.18

We then studied expression of p53 and p21, cell-cycle kinetics and apoptosis.

Gene_expression (expression) of p21 associated with apoptosis

2) Confidence 0.76 Published 2002 Journal Carcinogenesis Section Abstract Doc Link 12151346 Disease Relevance 1.30 Pain Relevance 0.19

While these investigators also showed that p21 levels were reduced in Pkd1 knockout mice, there has to our knowledge been no published study examining the function of p21 in human tissues or in non-knockout animal models of this disease.

Gene_expression (levels) of p21 associated with targeted disruption and disease

3) Confidence 0.75 Published 2007 Journal BMC Nephrol Section Body Doc Link PMC2045080 Disease Relevance 0.81 Pain Relevance 0

Expression of p21 in cortical homogenates from control and cystic Han:SPRD animals was assessed by immunoblotting (Fig. 3).

Gene_expression (Expression) of p21

4) Confidence 0.75 Published 2007 Journal BMC Nephrol Section Body Doc Link PMC2045080 Disease Relevance 0.53 Pain Relevance 0

Antisense p21 ODN transfection of MDCK cells at 400 and 600 nM showed increased cell proliferation as compared to cells transfected with scrambled control ODN (Fig. 5b).

Gene_expression (transfection) of p21 in MDCK

5) Confidence 0.75 Published 2007 Journal BMC Nephrol Section Body Doc Link PMC2045080 Disease Relevance 0.27 Pain Relevance 0

Consistent with this finding, our data indicate reduced expression of p21 in cystic kidneys from male, but not female, Han:SPRD rats.

Gene_expression (expression) of p21 associated with cystic kidney diseases

6) Confidence 0.75 Published 2007 Journal BMC Nephrol Section Body Doc Link PMC2045080 Disease Relevance 0.57 Pain Relevance 0

However, our finding that p21 is decreased in ADPKD, while in agreement with earlier studies showing that PKD1-deficient cells express lower p21, is not in complete concordance with the mTOR inhibitor data.

Gene_expression (express) of p21 associated with polycystic kidney disease

7) Confidence 0.75 Published 2007 Journal BMC Nephrol Section Body Doc Link PMC2045080 Disease Relevance 1.37 Pain Relevance 0

Our present findings that ADPKD, which is characterized by increased RTE cell growth, may in fact be a disease which results from diminished p21 expression in affected tissues are consistent with recent data showing that polycystin-1 induces p21 [9] and causes resistance to apoptosis [23].

Gene_expression (expression) of p21 associated with polycystic kidney disease, apoptosis and disease

8) Confidence 0.75 Published 2007 Journal BMC Nephrol Section Body Doc Link PMC2045080 Disease Relevance 0.70 Pain Relevance 0

In this study, we examine the expression of p21 in human tissue as well as in rat tissues.

Spec (examine) Gene_expression (expression) of p21

9) Confidence 0.75 Published 2007 Journal BMC Nephrol Section Body Doc Link PMC2045080 Disease Relevance 0.77 Pain Relevance 0

In contrast, renal p21 expression in cystic females did not differ from that seen in noncystic control females.

Gene_expression (expression) of p21

10) Confidence 0.75 Published 2007 Journal BMC Nephrol Section Body Doc Link PMC2045080 Disease Relevance 0.41 Pain Relevance 0

Renal expression of p21 was significantly attenuated in cystic male rats, as compared with noncystic controls (p < 0.01).

Gene_expression (expression) of p21

11) Confidence 0.75 Published 2007 Journal BMC Nephrol Section Body Doc Link PMC2045080 Disease Relevance 0.44 Pain Relevance 0

To clarify the basis for cell killing during prostate cancer radiotherapy, we determined the IR-induced expression of several apoptotic- (bax, bcl-2, survivin and PARP) and G1-cell cycle checkpoint- (p53 and p21(WAF1/Cip1)) related proteins, in both normal (PrEC-epithelial and PrSC-stromal) and malignant (LNCaP, DU-145 and PC-3; all epithelial) prostate cells.

Gene_expression (expression) of p21 in PC-3 associated with malignant neoplastic disease, apoptosis and reprotox - general 1

12) Confidence 0.75 Published 2003 Journal Prostate Cancer Prostatic Dis. Section Abstract Doc Link 12664070 Disease Relevance 0.78 Pain Relevance 0.07

We observed that IR-induced bax and p21(WAF1/Cip1) protein expression were attenuated selectively in normal stromal and epithelial cell cultures, yet maintained their p53-dependency in malignant cell lines.

Gene_expression (expression) of p21 in epithelial cell associated with malignant neoplastic disease

13) Confidence 0.75 Published 2003 Journal Prostate Cancer Prostatic Dis. Section Abstract Doc Link 12664070 Disease Relevance 0.91 Pain Relevance 0.10

Terminal growth arrest was dose-responsive at 96 h following irradiation and associated with long-term expression of both p21(WAF1/Cip1) and p16(INK4a) genes.

Gene_expression (expression) of WAF1

14) Confidence 0.75 Published 2003 Journal Prostate Cancer Prostatic Dis. Section Abstract Doc Link 12664070 Disease Relevance 0.59 Pain Relevance 0.05

Terminal growth arrest was dose-responsive at 96 h following irradiation and associated with long-term expression of both p21(WAF1/Cip1) and p16(INK4a) genes.

Gene_expression (expression) of p21

15) Confidence 0.75 Published 2003 Journal Prostate Cancer Prostatic Dis. Section Abstract Doc Link 12664070 Disease Relevance 0.59 Pain Relevance 0.05

Inhibition of PKCbeta(1)-mediated overexpression of p21(waf1/cip1) partially reduced the anti-apoptotic effect of PKCbeta(1).

Gene_expression (overexpression) of waf1 associated with apoptosis

16) Confidence 0.75 Published 2002 Journal Oncogene Section Abstract Doc Link 12203123 Disease Relevance 0.89 Pain Relevance 0.19

We observed that IR-induced bax and p21(WAF1/Cip1) protein expression were attenuated selectively in normal stromal and epithelial cell cultures, yet maintained their p53-dependency in malignant cell lines.

Gene_expression (expression) of WAF1 in epithelial cell associated with malignant neoplastic disease

17) Confidence 0.75 Published 2003 Journal Prostate Cancer Prostatic Dis. Section Abstract Doc Link 12664070 Disease Relevance 0.91 Pain Relevance 0.10

To clarify the basis for cell killing during prostate cancer radiotherapy, we determined the IR-induced expression of several apoptotic- (bax, bcl-2, survivin and PARP) and G1-cell cycle checkpoint- (p53 and p21(WAF1/Cip1)) related proteins, in both normal (PrEC-epithelial and PrSC-stromal) and malignant (LNCaP, DU-145 and PC-3; all epithelial) prostate cells.

Gene_expression (expression) of WAF1 in PC-3 associated with malignant neoplastic disease, apoptosis and reprotox - general 1

18) Confidence 0.75 Published 2003 Journal Prostate Cancer Prostatic Dis. Section Abstract Doc Link 12664070 Disease Relevance 0.78 Pain Relevance 0.07

The expression of COX-1 and COX-2. and of two cyclin dependent kinase inhibitors, p21Cip1 and p27Kip1, was analysed by Western blotting and RT-PCR.

Spec (analysed) Gene_expression (expression) of p21Cip1

19) Confidence 0.75 Published 2003 Journal Dig Liver Dis Section Body Doc Link 14567460 Disease Relevance 0.08 Pain Relevance 0

Higher concentrations of indomethacin and dexamethasone also up-regulated p21Cip1/Waf1 expression in hBMSCs, and so did celecoxib on D1-cells.

Gene_expression (expression) of p21Cip1/Waf1 associated with dexamethasone

20) Confidence 0.75 Published 2007 Journal Biochem. Pharmacol. Section Abstract Doc Link 17714695 Disease Relevance 0.18 Pain Relevance 0.84

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