INT282439

Context Info Confidence 0.47 First Reported 2009 Last Reported 2009 Negated 4 Speculated 2 Reported most in Body Documents 1 Total Number 8 Disease Relevance 4.66 Pain Relevance 0.11

Anatomy Link Frequency brain 3 Mat1a (Mus musculus)

Pain Link Frequency Relevance Heat Hippocampus 16 94.24 adenocard 16 35.24 Spinal cord 8 25.00 Neurotransmitter 8 20.24 anesthesia 8 16.92

Disease Link Frequency Relevance Heat Disease 304 98.84 Death 8 98.08 Cognitive Disorder 56 97.48 Aging 152 95.80 Urological Neuroanatomy 8 95.00 Frailty 8 91.88 Targeted Disruption 24 80.16 Stress 8 67.12 Neuroblastoma 16 61.84 Hyperhomocysteinemia 40 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

In addition, it is still unknown whether alterations in AdoMet and AdoHcy levels are cause or consequence of AD pathology.

Spec (whether) Regulation (alterations) of AdoMet associated with disease

1) Confidence 0.47 Published 2009 Journal Neurol Sci Section Body Doc Link PMC2746292 Disease Relevance 0.87 Pain Relevance 0.03

In addition, we showed that cholesterol-containing, or DHA-enriched diets did not affect AdoMet or AdoHcy levels in brain tissue of APP/PS1 and wild-type mice.

Neg (not) Regulation (affect) of AdoMet in brain

2) Confidence 0.47 Published 2009 Journal Neurol Sci Section Body Doc Link PMC2746292 Disease Relevance 0.59 Pain Relevance 0

If the above-mentioned hypothesis is valid, and AdoMet remains unaltered in aged APP/PS1 mice compared to young APP/PS1 mice, because of less demand for methylation due to neurodegeneration, AdoHcy levels will also be expected to be decreased or unchanged.

Regulation (unaltered) of AdoMet

3) Confidence 0.20 Published 2009 Journal Neurol Sci Section Body Doc Link PMC2746292 Disease Relevance 0.61 Pain Relevance 0.05

The finding that cholesterol- or DHA-containing diets do not alter AdoMet or AdoHcy levels in brain tissue of either wild type or APP/PS1 mice, but in contrast do have effects on AD pathology and cerebral hemodynamics [7, 15, 29], suggests that cholesterol- or DHA-containing diets do not influence cerebral hemodynamics or AD pathology via the methylation cycle.

Neg (not) Regulation (alter) of AdoMet in brain associated with disease

4) Confidence 0.20 Published 2009 Journal Neurol Sci Section Body Doc Link PMC2746292 Disease Relevance 0.59 Pain Relevance 0

Neither a cholesterol- nor a DHA-containing diet did alter AdoMet or AdoHcy levels in brain tissue of 8- and 15-month-old APP/PS1 and wild-type mice as compared to the standard diet (data not shown).

Neg (Neither) Regulation (alter) of AdoMet in brain

5) Confidence 0.20 Published 2009 Journal Neurol Sci Section Body Doc Link PMC2746292 Disease Relevance 0.63 Pain Relevance 0

The finding that AdoMet levels significantly differ only in 15-month-old APP/PS1 mice compared to their wild-type littermates, but not in 8-month-old mice, also indicates that alterations in AdoMet levels are a consequence of AD pathology rather than a cause.

Regulation (alterations) of AdoMet associated with disease

6) Confidence 0.20 Published 2009 Journal Neurol Sci Section Body Doc Link PMC2746292 Disease Relevance 0.56 Pain Relevance 0

No changes in AdoMet and AdoHcy levels, and consequently in the ratio between AdoMet and AdoHcy levels (methylation index), were observed between 8-month-old wild type and APP/PS1 mice (Fig. 1a–c).

Neg (No) Regulation (changes) of AdoMet

7) Confidence 0.20 Published 2009 Journal Neurol Sci Section Body Doc Link PMC2746292 Disease Relevance 0.09 Pain Relevance 0

AdoMet and AdoHcy might also influence AD like pathology and vascular health [16] and it could be hypothesized that the above-mentioned nutritional components may influence and AdoMet and AdoHcy levels as well.

Spec (may) Regulation (influence) of AdoMet associated with disease

8) Confidence 0.20 Published 2009 Journal Neurol Sci Section Body Doc Link PMC2746292 Disease Relevance 0.73 Pain Relevance 0.04

General Comments

This test has worked.